Chemical Research in Chinese Universities

, Volume 35, Issue 2, pp 239–244 | Cite as

Estradiol Induced Estrogen Receptor-mediated Transcription and Expression of Aquaporin5

  • Wei Wei
  • Xi He
  • Xintong Liu
  • Chuanjian Lan
  • Jiang LiEmail author


In this study, we constructed the recombinant plasmid of pGL2/Aquaporin5(AQP5) promoter (pGL2/AQP5p) luciferase reporter, then found estradiol(E2) induced AQP5 promoter activation in a dose-dependent manner. Further, we identified endogenous estrogen receptors(ER), including ERα and ERβ, expressed in human submandibular gland(HSG) cells, which responded to E2. Then demonstrated by the stimulation of E2, AQP5 was upregulated in protein level, meanwhile AQP5 located in cytomembrane was elevated in immunofluorescence. Furthermore, we revealed the roles of ERα and ERβ in AQP5 upregulation by E2. When ERα and ERβ were overexpressed, the AQP5 transcription level and protein expression were augmented obviously. While when knockdown ER by ERα-shRNA or ERβ-shRNA, AQP5 transcription and expression attenuated. Moreover, we detected the effect of E2 in Sjogren’s syndrome(SS) mice model in vivo. SS mice models were constructed by injecting submandibular gland antigen immune induction combined with estrogen deprivation, which were administrated with saline and E2. The salivary secretion was decreased, and the AQP5 expression downregulated in the submandibular gland in the SS model group. When SS mice were administrated with E2, the salivary secretion was significantly increased, and the AQP5 expression upregulated in the submandibular gland. These results suggest E2 activates AQP5p transcription and upregulates AQP5 protein expression, and E2 promotes salivary secretion in SS model in vivo. Taken together, we provided the evidence that E2 increased salivary secretion by activating AQP5 transcription and expression.


Aquaporin 5(AQP5) Sjogren’s syndrome(SS) Estradiol 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. [1]
    Agre P., Angewandte Chemie—International Edition, 2004, 43, 4278CrossRefGoogle Scholar
  2. [2]
    Verkman A. S., Reviews of Physiology Biochemistry and Pharmacology, 2005, 155, 31CrossRefGoogle Scholar
  3. [3]
    Yool A. J., Current Pharmaceutical Design, 2007, 13, 3212CrossRefGoogle Scholar
  4. [4]
    Rojek A., Praetorius J., Frokiaer J., Nielsen S., Fenton R., Annual Review of Physiology, 2008, 70, 301CrossRefGoogle Scholar
  5. [5]
    Ishibashi K., Tanaka Y., Morishita Y., Biochim. Biophys., Acta, 2014, 1840, 1507CrossRefGoogle Scholar
  6. [6]
    Kazuo H., European Journal of Physiology, 2016, 468(4), 519CrossRefGoogle Scholar
  7. [7]
    Agre P., Kozono D., FEBS Letters, 2003, 555, 72CrossRefGoogle Scholar
  8. [8]
    Ishikawa Y., Cho G., Yuan Z., Journal of Pharmacological Sciences, 2006, 100, 495CrossRefGoogle Scholar
  9. [9]
    Ma T., Song Y., Gillespie A., The Journal of Biological Chemistry, 1999, 16, 274Google Scholar
  10. [10]
    Mariette X., Criswell L. A., The New England Journal of Medicine, 2018, 378(10), 931CrossRefGoogle Scholar
  11. [11]
    Muhammad S. S., Clara C., Jason P., Christine D., International Journal of Molecular Sciences, 2018, 19(11), 3392CrossRefGoogle Scholar
  12. [12]
    Delporte C., Bryla A., Perret J., International Journal of Molecular Sciences, 2016, 17, 166CrossRefGoogle Scholar
  13. [13]
    Ichiyama T., Nakatani E., Tatsumi K., Hideshima K., Urano T., Nariai Y., Sekine J., Journal of Oral Science, 2018, 60, 212CrossRefGoogle Scholar
  14. [14]
    Yoshimura S., Nakamura H., Horai Y., Nakajima H., Shiraishi H., Hayashi T., Takahashi T., Kawakami A., Modern Rheumatology, 2016, 26, 384CrossRefGoogle Scholar
  15. [15]
    Enger T. B., Aure M. H., Jensen J. L., Galtung H. K., Acta Odontol. Scand., 2014, 72, 549CrossRefGoogle Scholar
  16. [16]
    Pauliina P., Mikael L., Liisa V., Praseet P., Ann. N. Y. Acad. Sci., 2007, 1108, 426CrossRefGoogle Scholar
  17. [17]
    Wei W., Zhao C. Y., Liu S. T., Jiao X. F., Liu X. T., Lan C. J., Li J., Chem. Res. Chinese Universities, 2018, 34(6), 929CrossRefGoogle Scholar
  18. [18]
    Nilsson S., Physiological Revews, 2001, 81, 1535CrossRefGoogle Scholar
  19. [19]
    Nemere I., Journal of Cellular Biochemistry, 2003, 88, 438CrossRefGoogle Scholar
  20. [20]
    Ishikawa Y., Yuan Z., Inoue N., Skowronski M. T., Nakae Y., Shono M., Am. J. Physiol. Cell Physiol., 2005, 289, 1303CrossRefGoogle Scholar
  21. [21]
    Porola P., Laine M., Virkki L., Poduval P., Konttinen Y. T., Annals of The New York Academy of Sciences, 2007, 1108(1), 426CrossRefGoogle Scholar

Copyright information

© Jilin University, The Editorial Department of Chemical Research in Chinese Universities and Springer-Verlag GmbH 2019

Authors and Affiliations

  • Wei Wei
    • 1
  • Xi He
    • 1
  • Xintong Liu
    • 1
  • Chuanjian Lan
    • 1
  • Jiang Li
    • 1
    Email author
  1. 1.Dental HospitalJilin UniversityChangchunP. R. China

Personalised recommendations