Artificial Esterase Based on Self-assembly Gel Microspheres Constructed from Chitosan and Amino Acids

  • Jing Cao
  • Miao WangEmail author
  • Weihua Chen
  • Yongxin She
  • Jing WangEmail author
  • Fengzhong Wang
  • Shuibing Lao


A novel artificial esterase based on chitosan and amino acids was synthesized in the present study. The Fmoc-His and Glu were linked to chitosan by active ester method(AEM). The hydroxide radical in chitosan, imidazole group of Fmoc-His and carboxyl from Glu formed a catalytic center of natural esterase. Gel microspheres were coated with a protective layer and a supporting layer by self-assembly construction function in carboxymethylcellulose sodium(CMCS) solution. As for catalytic activity, chitosan-His-Glu was found to be more efficient than chitosan- His and chitosan-Glu in mimicking the core catalytic sites of natural esterase, and the best ratio(mass ratio) of chitosan-His-Glu:CMCS was 1:3. Furthermore, metal ions, such as Ca2+, Mg2+, Fe2+, etc., were able to improve the catalytic efficiency of artificial esterase. And the Lineweaver-Burk plot indicated that the catalytic kinetics of artificial esterase conformed to Michaelis-Menten equation.


Artificial enzyme Esterase Chitosan 


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  1. [1]
    Böcking A., Großarth C., Deimling O. V., Histochemistry, 1974, 42, 359CrossRefGoogle Scholar
  2. [2]
    Hou C. J., Huo D. Q., Yang M., Huang S., Zhang L., Shen C. H., World Journal of Microbiology & Biotechnology, 2012, 28, 541CrossRefGoogle Scholar
  3. [3]
    Tao Z., Han W. J., Liu Z. P., Microbial Cell Factories, 2009, 8, 67CrossRefGoogle Scholar
  4. [4]
    Parker M. D., Yoo D., Babiuk L. A., Journal of Virology, 1990, 64, 1625Google Scholar
  5. [5]
    Ma B. D., Kong X. D., Yu H. L., Zhang Z. J., Dou S., Xu Y. P., Ni Y., Xu J. H., ACS Catalysis, 2014, 4, 1026CrossRefGoogle Scholar
  6. [6]
    Vandersteen A. M., Han H., Janda K. D., Molecular Diversity, 1996, 2, 89CrossRefGoogle Scholar
  7. [7]
    Hammes G. G., Benkovic S. J., Hammesschiffer S., Biochemistry, 2011, 50, 10422CrossRefGoogle Scholar
  8. [8]
    Dong Z. Y., Zhu J. Y., Luo Q., Liu J. Q. Science China: Chemistry, 2013, 56, 1067CrossRefGoogle Scholar
  9. [9]
    Derakhshankhah H., Saboury A. A., Divsalar A., Mansouri-Torshizi H., Bamery I., Ajloo D., Moosavi-Movahedi A. A., Hosseinzadeh R., Ganjali M. R., Ilkhani H., Journal of the Iranian Chemical Society, 2014, 11, 1381CrossRefGoogle Scholar
  10. [10]
    Lv Y. Q., Wang M. F., Qi W., Su R. X., He Z. M., Chem. J. Chinese Universities, 2015, 36(7), 1304Google Scholar
  11. [11]
    Rufo C. M., Moroz Y. S., Moroz O. V., Stöhr J., Smith T. A., Hu X., Degrado W. F., Korendovych I. V., Nature Chemistry, 2014, 6, 303CrossRefGoogle Scholar
  12. [12]
    Zhang C., Xue X., Luo Q., Li Y., Yang K., Zhuang X., Jiang Y., Zhang J., Liu J., Zou G., ACS Nano, 2014, 8, 11715CrossRefGoogle Scholar
  13. [13]
    Wang Z. G., Wang H., Liu Q., Duan F., Shi X., Ding B., ACS Catalysis, 2018, 8, 7016CrossRefGoogle Scholar
  14. [14]
    Pokkuluri P. R., Duke N. E., Wood S. J., Cotta M. A., Li X. L., Biely P., Schiffer M., Proteins Structure Function & Bioinformatics, 2011, 79, 2588CrossRefGoogle Scholar
  15. [15]
    Mascini M., Sergi M., Monti D., Del C. M., Compagnone D., Analytical Chemistry, 2008, 80, 9150CrossRefGoogle Scholar
  16. [16]
    Lu L., Wei C., Advanced Materials, 2010, 22, 3745CrossRefGoogle Scholar
  17. [17]
    Li M. Y., Li Y. J., Chen J. J., Lv W., Pan X. W., Liu J., Liu Q. Y., Wei L., Zhang L. R., Yang X. D., Lu J. F., Wang K., Biochemistry, 2007, 46, 11451CrossRefGoogle Scholar
  18. [18]
    Paletta-Silva R., Vieira-Bernardo R., Cosentino-Gomes D., Meyer- Fernandes J. R., Experimental Parasitology, 2012, 131, 63CrossRefGoogle Scholar
  19. [19]
    Li J. S., Li J. L., Wu T. T., Journal of Fish Biology, 2010, 71, 1788CrossRefGoogle Scholar
  20. [20]
    Raykova D., Dorovska-Taran V., Blagoev B., Biochimie, 1981, 63, 397CrossRefGoogle Scholar

Copyright information

© Jilin University, The Editorial Department of Chemical Research in Chinese Universities and Springer-Verlag GmbH 2019

Authors and Affiliations

  1. 1.Institute of Quality Standardization & Testing Technology for Agro-productsChinese Academy of Agricultural SciencesBeijingP. R. China
  2. 2.Key Laboratory of Agrofood Safety and Quality(Beijing)Ministry of AgricultureBeijingP. R. China
  3. 3.Institute of Agro-products Processing Science and TechnologyChinese Academy of Agricultural SciencesBeijingP. R. China
  4. 4.Agro-products Quality Safety and Testing Technology Research InstituteGuangxi Academy of Agricultural SciencesNanningP. R. China

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