Chemical Research in Chinese Universities

, Volume 34, Issue 6, pp 893–898 | Cite as

Eco-friendly and Cleaner Process Using Online Microwave-assisted Steam Extraction Coupled with Solid-phase Extraction for Trace Analysis of Sulfonamides in Animal Feed

  • Guijie Li
  • Chang Liu
  • Dawei WangEmail author
  • Lan DingEmail author


An environmentally friendly method for extracting sulfonamides(SAs) residues from animal feed was described and applied. The method used online microwave-assisted steam extraction coupled with solid phase extraction(MASE-SPE), which was followed by the analysis using high performance liquid chromatography-mass spectrometry(HPLC-MS/MS). The SAs residues were extracted successively with water steam under microwave irradiation, and thus directly introduced into an SPE column containing cation-exchange resin. The SAs were then eluted with methanol-ammonia(90:10, volume ratio) from the SPE column and followed by HPLC-MS/MS. The limits of detection(LODs) for the analytes ranged from 0.24 ng/g to 0.49 ng/g. The limits of quantification(LOQs) ranged from 0.82 ng/g to 1.63 ng/g. Average recoveries of SAs were 76.3%―92.1%. The developed method was a reliable and environmentally friendly alternative to previous methods with respect to time, solvent and labor consumption for the analysis of SAs in animal foodstuffs.


High performance liquid chromatography-mass spectrometry Microwave-assisted steam extraction Solid-phase extraction Sulfonamide Animal feed 


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  1. [1]
    Tadi K. K., Motghare R. V., Ganesh V., Electroanalysis, 2014, 26, 2328CrossRefGoogle Scholar
  2. [2]
    Vian M. A., Fernandez X., Visinoni F., Chemat F., J. Chromatogr. A, 2008, 1190, 14CrossRefGoogle Scholar
  3. [3]
    Adrian J., Pasche S., Voirin G., Adrian J., Pinacho D. G., Font H., J. Trends Anal. Chem., 2009, 28, 769CrossRefGoogle Scholar
  4. [4]
    Fu X., Liang H., Xia B., Huang C., Ji B., Zhou Y., J. Agric. Food Chem., 2017, 65, 8256CrossRefGoogle Scholar
  5. [5]
    Yu W., Li K., Liu Z., Zhang H., Jin X., Microchem. J., 2018, 136, 263CrossRefGoogle Scholar
  6. [6]
    Kechagia M., Samanidou V., Kabir A., Furton K. G., J. Sep. Sci., 2018, 41, 723CrossRefGoogle Scholar
  7. [7]
    Dai T., Duan J., Li X., Xu X., Shi H., Kang W., Int. J. Mol. Sci., 2017, 18, 1286CrossRefGoogle Scholar
  8. [8]
    Abian J., Churchwell M. I., Korfmacher W. A., J. Chromatogr. A, 1993, 629, 267CrossRefGoogle Scholar
  9. [9]
    Wang Y., Liu L., Xiao C., Chen L., Yang P., Liu Q., Wang J., Liu X., Food Anal. Method, 2016, 9, 2521CrossRefGoogle Scholar
  10. [10]
    Abdallah H., Arnaudguilhem C., Lobinski R., Jaber F., Anal. Methods, 2015, 7, 1549CrossRefGoogle Scholar
  11. [11]
    Li J., Liu H., Zhang J., Liu Y., Wu L., Biomed. Chromatogr., 2016, 30, 1331CrossRefGoogle Scholar
  12. [12]
    Chen R., Yang Y., Wang N., Hao L., Li L., Guo X., Zhang J., Hu Y., Shen W., J. Sep. Sci., 2015, 38, 749CrossRefGoogle Scholar
  13. [13]
    Wang Y., Liu L., Xiao C., Chen L., Yang P., Liu Q., Liu X., Food Anal. Method, 2016, 9, 2521CrossRefGoogle Scholar
  14. [14]
    Robert C., Gillard N., Brasseur P. Y., Ralet N., Dubois M., Delahaut P., Food Control, 2015, 50, 509CrossRefGoogle Scholar
  15. [15]
    Muriano A., Pinacho D. G., Chabottaux V., Diserens J. M., Granier B., Stead S., Sanchez B. F., Pividori M. I., Marco M. P., Anal. Bioanal. Chem., 2013, 405, 7885CrossRefGoogle Scholar
  16. [16]
    Kim H. J., Jeong M. H., Park H. J., Kim W. C., Kim J. E., Food Chem., 2016, 196, 1144CrossRefGoogle Scholar
  17. [17]
    Haller M. Y., Müller S. R., McArdell C. S., Alder A. C., Suter M. J. F., J. Chromatogr. A, 2002, 952, 111CrossRefGoogle Scholar
  18. [18]
    Kantiani L., Farré M., Barceló D., J. Chromatogr. A, 2010, 1217, 4247CrossRefGoogle Scholar
  19. [19]
    LeDoux M., J. Chromatogr A, 2011, 1218, 1021CrossRefGoogle Scholar
  20. [20]
    Kishida K., Furusawa N. J., J. Chromatogr. A, 2001, 937, 49CrossRefGoogle Scholar
  21. [21]
    Herrero M., Mendiola J. A., Cifuentes A., Ibáñez E., J. Chromatogr. A, 2010, 1217, 2495CrossRefGoogle Scholar
  22. [22]
    Wang H., Xu Y., Song W., Zhao Q., Zhang, X., Zeng Q., J. Sep. Sci., 2011, 34, 2489CrossRefGoogle Scholar
  23. [23]
    Balakrishnan V. K., Exall K. N., Toito J. M., Can. J. Chem. Eng., 2014, 92, 369CrossRefGoogle Scholar
  24. [24]
    Purcaro G., Moret S., Conte L. S., Meat. Sci., 2009, 81, 275CrossRefGoogle Scholar
  25. [25]
    Miyawaki T., Tobiishi K., Takenaka S., Kadokami K., Soil Sediment Contam., 2018, 27, 31CrossRefGoogle Scholar
  26. [26]
    Pan X., Niu G., Liu H., J. Chromatogr. A, 2001, 922, 371CrossRefGoogle Scholar
  27. [27]
    Ferreres F., Grosso C., Gil-Izquierdo A., Valentão P., Mota A. T., Andrade P. B., Food Chem., 2017, 230, 463CrossRefGoogle Scholar
  28. [28]
    Iglesias-García I., Barriada-Pereira M., González-Castro M. J., Muniategui-Lorenzo S., López-Mahía P., Prada-Rodríguez D., J. Anal. Bioanal. Chem., 2008, 391, 745CrossRefGoogle Scholar
  29. [29]
    Wang Z. M., Ding L., Wang L., Feng J., Li T. C., Zhou X., Zhang H. Q., Chinese J. Chem., 2006, 24, 649CrossRefGoogle Scholar
  30. [30]
    Abert V. M., Maingonnat J. F., Chemat F., J. Chromatogr. A, 2009, 1216, 7700CrossRefGoogle Scholar
  31. [31]
    Ferhat M. A., Tigrine-Kordjani N., Chemat S., Meklati B. Y., Chemat F., Chromatographia, 2007, 65, 217CrossRefGoogle Scholar
  32. [32]
    Périno-Issartier S., Abert-Vian M., Petitcolas E., Chemat F., Chro-matographia, 2010, 72, 347CrossRefGoogle Scholar
  33. [33]
    Vian M. A., Fernandez X., Visinonic F., Chemat F., J. Chromatogr. A, 2008, 1190, 14CrossRefGoogle Scholar
  34. [34]
    Numata M., Yarita T., Aoyagi Y., Takatsu A., J. Anal. Chem., 2003, 75, 1450CrossRefGoogle Scholar
  35. [35]
    Song W., Zhang Y., Li G., Chen H., Wang H., Zhao Q., J. Food. Chem., 2014, 143, 192CrossRefGoogle Scholar
  36. [36]
    Čizmić M., Babić S., Kaštelan-Macan M., Environ. Sci. Pollut. R., 2017, 24, 20521CrossRefGoogle Scholar
  37. [37]
    Liu R., He P., Li Z., Li R., J. Chromatogr. Sci., 2011, 49, 640CrossRefGoogle Scholar
  38. [38]
    Kim H. J., Jeong M.H., Park H. J., Kim W. C., Kim J. E., J. Food. Chem., 2016, 96, 1144CrossRefGoogle Scholar
  39. [39]
    Yu W., Liu Z., Gao S., Cui S., Yang X., Qiu W., Zhang H., Yu A., Huan Y., Anal. Methods, 2013, 5, 5983CrossRefGoogle Scholar

Copyright information

© Jilin University, The Editorial Department of Chemical Research in Chinese Universities and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.College of Food ScienceJilin Agricultural UniversityChangchunP. R. China
  2. 2.College of ChemistryJilin UniversityChangchunP. R. China
  3. 3.Jilin Province Product Quality Supervision and Inspection InstituteChangchunP. R. China

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