Chemical Research in Chinese Universities

, Volume 34, Issue 4, pp 523–527 | Cite as

Development of a Two-photon Ratiometric Fluorescent Probe for Glutathione and Its Applications in Living Cells

  • Gaoping Xu
  • Yonghe Tang
  • Weiying LinEmail author


Glutathione(GSH), as the most abundant intracellular biothiol, plays an important role in the redox homeostasis of the organism. Abnormal concentrations of GSH in cells may lead to many malignant diseases, such as cancer, liver damage and neurodegenerative diseases. It is urgent to develop effective methods to detect GSH in living organisms. In this work, a new two-photon ratiometric fluorescent probe Co-GSH based on the coumarinchalcone dye platform was judiciously developed. Based on the Michael-addition reaction, Co-GSH was able to identify GSH with high selectivity and sensitivity. Furthermore, assisted by laser-scanning confocal microscopy, Co-GSH could specifically response GSH over the other biothiols, including Cys and Hcy, in living HeLa cells by using one- and two-photon modes.


Fluorescence probe Glutathione Michael-addition reaction Two-photon Ratiometric 


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Supplementary material

40242_2018_8089_MOESM1_ESM.pdf (1 mb)
Development of a two-photon ratiometric fluorescent probe for glutathione and its applications in living cells


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Copyright information

© Jilin University, The Editorial Department of Chemical Research in Chinese Universities and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Institute of Fluorescent Probes for Biological Imaging, School of Materials Science and Engineering, School of Chemistry and Chemical EngineeringUniversity of JinanJinanP. R. China

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