Advertisement

Chemical Research in Chinese Universities

, Volume 34, Issue 4, pp 590–597 | Cite as

Metabonomics and Molecular Biology-based Effects of Sugemule-3 in an Isoproterenol-induced Cardiovascular Disease Rat Model

  • Xiye Wang
  • Yu Wang
  • Chengxi Wei
  • Lijun Yu
Article
  • 3 Downloads

Abstract

Cardiovascular disease(CVD) is a common and serious disease in the elderly, which has characteristically high prevalence, disability, and mortality rates. However, the etiology of CVD is still not very clear. The traditional Mongolian medicine Sugemule-3(SM) is usually used for the treatment of CVD and exhibits a good curative effect. In this study, a serum metabolite profile analysis was used to identify potential biomarkers associated with isoproterenol(ISO)-induced CVD and investigate the mechanism of the action of SM. Ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS) was used for the metabonomics analysis. Principal component analysis(PCA) was used to process the acquired data to differentiate the results of the control, CVD, and SM treatment groups. Orthogonal partial least squares discriminant analysis(OPLS-DA) enabled the identification of 21 metabolites as potential biomarkers that were relevant to phospholipid and energy metabolism. The results indicate that SM played a protective role against ISO-induced CVD in rats by regulating phospholipid and energy metabolic pathways. Further, we verified the apoptotic metabolic pathway using molecular biology methods, such as terminal deoxynucleotidyl transferase(TdT) deoxyuridine 5′-triphosphate(dUTP) nick-end labeling(TUNEL) assay and Western blot analysis. Furthermore, this study identified early biomarkers of CVD and elucidated the underlying mechanism of the therapeutic actions of SM, which is worth further to be investigated for development as a clinical therapy.

Keywords

Sugemule-3 Metabonomics Molecular biology Ultra-performance liquid chromatography coupled with mass spectrometry(UPLC-MS) Cardiovascular disease Isoproterenol 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. [1]
    Esposito K., Giugliano D., N. Engl. J. Med., 2013, 369, 674Google Scholar
  2. [2]
    Perk J., de Backer G., Gohlke H., Turk. Kardiyol. Dern. Ars., 2012, 40, 1CrossRefGoogle Scholar
  3. [3]
    Mann D. L., Kent R. L., Parsons B., Cooper G., Circulation, 1992, 85, 790CrossRefGoogle Scholar
  4. [4]
    Bovo E., Lipsius S. L., Zima A. V., J. Physiol., 2012, 590, 3291CrossRefGoogle Scholar
  5. [5]
    Pollack M., Phaneuf S., Dirks A., Leeuwenburgh C., Ann. N. Y. Acad. Sci., 2002, 959, 93CrossRefGoogle Scholar
  6. [6]
    Chang Y., Zhang P., Zhang X., Nat. Prod. Res., 2016, 12, 1Google Scholar
  7. [7]
    Nimaradan U., Four Medical Tantras, 2nd Ed., People’s Medical Publishing House, Beijing, 1983Google Scholar
  8. [8]
    Wang Y., Gong G. H., Xu Y. N., Yu L. J., Wei C. X., Pharmacognosy Magazine, 2017, 51, 517Google Scholar
  9. [9]
    Zhao Y. Y., Lei P., Chen D. Q., Feng Y. L., Bai X., J. Pharm. Biomed. Anal., 2013, 81, 202Google Scholar
  10. [10]
    Wang X. Y., Shan X. T., Wang Y. L., Li D., Zhao M., Xu L., Chem. J. Chinese Universities, 2016, 37(5), 844Google Scholar
  11. [11]
    Dai W., Wei C., Kong H., Jia Z., Han J., Zhang F., Wu Z., Gu Y., Chen S., Gu Q., Lu X., WuY., Xu G., J. Pharm. Biomed. Anal., 2011, 56, 86CrossRefGoogle Scholar
  12. [12]
    Zeng Q., Feng J. J., Lu T., Xu L. Y., Min C. Y., Xie H. P., Chem. Res. Chinese Universities, 2017, 33(1), 17CrossRefGoogle Scholar
  13. [13]
    Li P. H., Deng L. L., Luo J., Li W., Ning J., Ding J. H., Wu X. P., Chem. J. Chinese Universities, 2016, 37(4), 626Google Scholar
  14. [14]
    Zhang R. X., Liu S., Pi Z. F., Song F. R., Liu Z. Q., Chem. J. Chinese Universities, 2014, 35(6), 1146Google Scholar
  15. [15]
    Wang Y., Gong G. H., Zhou W., Zhang B., Bao S. Y., Wei C. X., Yue J. J., Zhang Y. F., Molecules, 2014, 19, 18090CrossRefGoogle Scholar
  16. [16]
    Deng W. K., Wang Y. B., Liu Z. X., Cheng H., Xue Y., PLoS One, 2014, 9, e111988CrossRefGoogle Scholar
  17. [17]
    Qi Y., Li S. Z., Pi Z. F., Song F. R., Lin N., Liu S. Y., Liu Z. Q., J. Chromatogr. B, 2014, 953, 11CrossRefGoogle Scholar
  18. [18]
    Astrid E. A., Stephan L. M., Martin C. M., Br. J. Pharmacol., 2004, 143, 666CrossRefGoogle Scholar
  19. [19]
    Hla T., Semin. Cell Dev. Biol., 2004, 15, 513CrossRefGoogle Scholar
  20. [20]
    Pyne S., Pyne N. J., Biochem. J., 2000, 349, 385CrossRefGoogle Scholar
  21. [21]
    Spiegel S., Kolesnick R., Leukemia, 2002, 16, 1596CrossRefGoogle Scholar
  22. [22]
    Olivier C., Grisha P., Burkhard K., Vanek P. G., Coso O. A., Gutkind S., Spiegel S., Nature, 1996, 381, 800CrossRefGoogle Scholar
  23. [23]
    Kikkawa U., Takai Y., Minakuchi R., Inohara S., Nishizuka Y., J. Biol. Chem., 1982, 257, 13341Google Scholar
  24. [24]
    Vanna C., Manuela V., Sandro S., Eur. J. Biochem., 1994, 221, 1095CrossRefGoogle Scholar
  25. [25]
    Hsieh C. C., Yen M. H., Liu H. W., Lau Y. T., Atherosclerosi, 2000, 151, 481CrossRefGoogle Scholar
  26. [26]
    Rikke N., Tae-Hwan K., JØrgen F., Kidney Res. Clin. Pract., 2015, 34, 194CrossRefGoogle Scholar
  27. [27]
    Mederle K., Meurer M., Castrop H., Höcherl K., Am. J. Physiol. Renal Physiol., 2015, 309, 332CrossRefGoogle Scholar
  28. [28]
    Sklyarov A.Y., Panasyuk N. B., Fomenko I. S., J. Physiol. Pharma-col., 2011, 62, 65Google Scholar
  29. [29]
    Chang J., Choi J., Rah Y. C., Too M. H., Oh K. H., Im G. J., Lee S. H., Kwon S. Y., Park H. C., Chae S. W., Jung H. H., PLoS One, 2016, 11, e0151557CrossRefGoogle Scholar
  30. [30]
    Green D. R., Reed J. C., Science, 1998, 281, 1309CrossRefGoogle Scholar
  31. [31]
    Adams J. M., Cory S., Science, 1998, 281, 1322CrossRefGoogle Scholar
  32. [32]
    Kang P. M., Izumo S., Circ. Res., 2000, 86, 1107CrossRefGoogle Scholar

Copyright information

© Jilin University, The Editorial Department of Chemical Research in Chinese Universities and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Inner Mongolia Key Laboratory for the Natural Products Chemistry and Functional Molecular Synthesis, College of Chemistry and Chemical EngineeringInner Mongolia University for the NationalitiesTongliaoP. R. China
  2. 2.Inner Mongolia Autonomous Region Key Laboratory of Mongolian Medicine Pharmacology for Cardio-cerebral Vascular System, Medicinal Chemistry and Pharmacology InstituteInner Mongolia University for the NationalitiesTongliaoP. R. China

Personalised recommendations