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Chemical Research in Chinese Universities

, Volume 30, Issue 2, pp 289–292 | Cite as

Enzymatic promiscuity: Escherichia coli BioH esterase-catalysed Aldol reaction and Knoevenagel reaction

  • Ling Jiang
  • Hongwei YuEmail author
Article

Abstract

Esterase BioH, which is obligatory for biotin synthesis in Escherichia coli, was found to exhibit a promiscuous ability to catalyse Aldol and Knoevenagel reactions with moderate to good yields. The reaction conditions including organic solvent, molar ratio of ketone to aldehyde, enzyme amount, and reaction time were investigated to evaluate the effect of different reaction conditions on yield. Target compounds were afforded in the best yield of 91.2% for Aldol reaction and 54.7% for Knoevenagel reaction. In addition, because the enzyme could be prepared with a low cost, this protocol could provide an economic route to conduct Aldol and Knoevenagel reactions, which expand the field of enzymatic promiscuity.

Keywords

Escherichia coli BioH esterase Catalytic promiscuity Aldol reaction Knoevenagel reaction 

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Copyright information

© Jilin University, The Editorial Department of Chemical Research in Chinese Universities and Springer-Verlag GmbH 2014

Authors and Affiliations

  1. 1.Department of Chemical and Biological EngineeringZhejiang UniversityHangzhouP. R. China

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