Phytoestrogens inhibiting androgen receptor signal and prostate cancer cell proliferation
- 133 Downloads
The androgen receptor(AR) signaling activated by dihydrotestosterone(DHT) plays critical roles in prostate cancer development and progression. Phytoestrogens, which are diphenolic compounds with estrogen and antiestrogen effects, can bind to estrogen receptors. However, their function on AR signaling has not been fully elucidated. In this study, dual-luciferase reporter assay, immunobloting, docking system test, MTT assay, immunofluorescence and chromatin immunoprecipitation(ChIP) assays were employed to examine the potential effects of three phytoestrogens(genistein, daidzein, flavone) on DHT-activated prostate specific antigen(PSA) activation, cell proliferation and AR transactivation in lymph node carcinoma of prostate(LNCaP) cells. Phytoestrogens were detected to down-regulate DHT-activated AR-mediated PSA promoter transactivation by dual-luciferase reporter system. Furthermore, three phytoestrogens, especially genistein, were demonstrated to significantly decrease AR-activated PSA protein expression by Western blotting analysis. MTT experiment proves that phytoestrogens, especially genistein, remarkably inhibits the DHT-induced cell proliferation in LNCaP cells. To provide reasonable explanations for experimental phenomena mentioned above, we did docking system test and detected phytoestrogens to share the same AR-binding site with DHT. To further prove the competition between phytoestrogen and DHT on AR binding, we examined the effects of phytoestrogens on DHT-activated AR nuclear translocation and immunofluorescence analysis which confirms that phytoestrogens, especially genistein, inhibit DHT-activated androgen receptor nuclear translocation. Results from ChIP show that phytoestrogens down-regulate DHT-induces AR binding to the androgen response elements(AREs, including AREI, AREII, and AREIII) in PSA promoter. Genistein remarkably down-regulates AR, binding to the AREI located in −250— −39 bp and AREIII in −4170— −3978 bp in the presence of DHT. In general, three phytoestrogens were identified to inhibit DHT-AR binding by competitively binding to AR and inhibit AR-mediated transactivation. And genistein shows the strongest effects among three phytoestrogens. Our findings confirm that phytoestrogens are AR antagonist in the regulation of AR-related PSA activation and cell proliferation, which provides valuable insights into the treatment of prostate cancer.
KeywordsAndrogen receptor Phytoestrogen Prostate specific antigen(PSA) Prostate cancer
Unable to display preview. Download preview PDF.
- Vernon S. E., Williams W. D., The Journal of Urology, 1983, 130(1), 95Google Scholar
- Trott O., Olson A. J., Journal of Computational Chemistry, 2010, 31, 455Google Scholar
- Li X. M., Xu Y., Tsuji I., Soybean and Prostate Cancer, InTech Publishiug, Rijeka, 2013, 265Google Scholar
- Liu G. F., Gao L. Z., Yu Q., Zhang H. M., Wang Z. X., Chem. J. Chinese Universities, 2012, 33(9), 1920Google Scholar
- Xie J., Zhu J. H., Chem. J. Chinese Universities, 2011, 32(7), 1532Google Scholar
- Zhou J. R., Gugger E. T., Tanaka T., Guo Y., Blackburn G. L., Clinton S. K., The Journal of Nutrition, 1999, 129(9), 1628Google Scholar