Chemical Research in Chinese Universities

, Volume 29, Issue 5, pp 911–916

Phytoestrogens inhibiting androgen receptor signal and prostate cancer cell proliferation

  • Jing Wu
  • Shu Liu
  • Xiao-yan Shen
  • Nan-yang Yang
  • Ying Liu
  • Ichiro Tsuji
  • Takaki Yamamura
  • Jiang Li
  • Xiao-meng Li
Article

DOI: 10.1007/s40242-013-3123-6

Cite this article as:
Wu, J., Liu, S., Shen, X. et al. Chem. Res. Chin. Univ. (2013) 29: 911. doi:10.1007/s40242-013-3123-6

Abstract

The androgen receptor(AR) signaling activated by dihydrotestosterone(DHT) plays critical roles in prostate cancer development and progression. Phytoestrogens, which are diphenolic compounds with estrogen and antiestrogen effects, can bind to estrogen receptors. However, their function on AR signaling has not been fully elucidated. In this study, dual-luciferase reporter assay, immunobloting, docking system test, MTT assay, immunofluorescence and chromatin immunoprecipitation(ChIP) assays were employed to examine the potential effects of three phytoestrogens(genistein, daidzein, flavone) on DHT-activated prostate specific antigen(PSA) activation, cell proliferation and AR transactivation in lymph node carcinoma of prostate(LNCaP) cells. Phytoestrogens were detected to down-regulate DHT-activated AR-mediated PSA promoter transactivation by dual-luciferase reporter system. Furthermore, three phytoestrogens, especially genistein, were demonstrated to significantly decrease AR-activated PSA protein expression by Western blotting analysis. MTT experiment proves that phytoestrogens, especially genistein, remarkably inhibits the DHT-induced cell proliferation in LNCaP cells. To provide reasonable explanations for experimental phenomena mentioned above, we did docking system test and detected phytoestrogens to share the same AR-binding site with DHT. To further prove the competition between phytoestrogen and DHT on AR binding, we examined the effects of phytoestrogens on DHT-activated AR nuclear translocation and immunofluorescence analysis which confirms that phytoestrogens, especially genistein, inhibit DHT-activated androgen receptor nuclear translocation. Results from ChIP show that phytoestrogens down-regulate DHT-induces AR binding to the androgen response elements(AREs, including AREI, AREII, and AREIII) in PSA promoter. Genistein remarkably down-regulates AR, binding to the AREI located in −250— −39 bp and AREIII in −4170— −3978 bp in the presence of DHT. In general, three phytoestrogens were identified to inhibit DHT-AR binding by competitively binding to AR and inhibit AR-mediated transactivation. And genistein shows the strongest effects among three phytoestrogens. Our findings confirm that phytoestrogens are AR antagonist in the regulation of AR-related PSA activation and cell proliferation, which provides valuable insights into the treatment of prostate cancer.

Keywords

Androgen receptor Phytoestrogen Prostate specific antigen(PSA) Prostate cancer 

Copyright information

© Jilin University, The Editorial Department of Chemical Research in Chinese Universities and Springer-Verlag GmbH 2013

Authors and Affiliations

  • Jing Wu
    • 1
  • Shu Liu
    • 1
  • Xiao-yan Shen
    • 1
  • Nan-yang Yang
    • 1
  • Ying Liu
    • 2
  • Ichiro Tsuji
    • 3
  • Takaki Yamamura
    • 4
  • Jiang Li
    • 2
  • Xiao-meng Li
    • 1
  1. 1.Key Laboratory of Molecular Epigenetics, Ministry of Education, Institute of Genetics and CytologyNortheast Normal UniversityChangchunP. R. China
  2. 2.Dental HospitalJilin UniversityChangchunP. R. China
  3. 3.Department of Public HealthTohoku UniversitySendaiJapan
  4. 4.Department of Nutritional SciencesMorioka UniversityMoriokaJapan

Personalised recommendations