Chemical Research in Chinese Universities

, Volume 29, Issue 3, pp 434–438 | Cite as

Determination of amino acids in Panax notoginseng by microwave hydrolysis and derivatization coupled with capillary zone electrophoresis detection

  • Xiao-tian Li
  • Ya-jing Zhao
  • Cheng-fei Jiang
  • Han-qi Zhang
  • Ai-min Yu
Article
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Abstract

The microwave hydrolysis and derivatization coupled with capillary electrophoresis detection were developed for the separation and determination of the amino acids in Panax notoginseng. The experimental conditions for the microwave hydrolysis and derivatization were examined and optimized. Several parameters of capillary electrophoresis, such as pH value of background electrolyte, borate concentration and applied voltage were optimized. Under the selected conditions, 11 amino acids were completely separated. The real sample was analyzed and the results were satisfactory. Compared with that of conventional heat hydrolysis and derivatization, the analytical time of this method was significantly shortened.

Keywords

Microwave hydrolysis Derivatization Amino acid Capillary electrophoresis Panax notoginseng 2,4-Dinitrofluorobenene 
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Copyright information

© Jilin University, The Editorial Department of Chemical Research in Chinese Universities and Springer-Verlag GmbH 2013

Authors and Affiliations

  • Xiao-tian Li
    • 1
  • Ya-jing Zhao
    • 1
  • Cheng-fei Jiang
    • 1
  • Han-qi Zhang
    • 1
  • Ai-min Yu
    • 1
  1. 1.College of ChemistryJilin UniversityChangchunP. R. China

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