Optimum removal conditions of aniline compounds in simulated wastewater by laccase from white-rot fungi

  • Bo YangEmail author
  • Yacheng Wang
  • Zhiguo Liu
  • Jun Liu
  • Jiaming Cai
Research Article



Aniline compounds are widely applied as important chemical raw materials. However, they are so toxic and harmful to humans and environment that they need to be removed by an effective and economic approach, such as enzymatic reaction, which is in line with contemporary green development concepts.


The effects of major factors, such as temperature, reaction time, concentration of laccase and the initial concentration of substrate on the removal of substrate were investigated by OFAT approach. After simulated wastewater is treated with enzymes, aniline concentration was determined by N-(1-Naphthyl)ethylene-diamine dihydrochloride spectrophotometric method. Concentration of o-phenylenediamine was determined by ferric ammonium alum spectrophotometric method.


For the removal of aniline, the optimum conditions were as follows: 50 °C, initial aniline concentration of 80 mg/L and laccase concentration of 1 g/L. In this case, the total removal of aniline reached 97.1% after 8 h, this also involves the volatilization of aniline itself. The optimum conditions of o-phenylenediamine were as follows: 50 °C, initial concentration of 100 mg/L and laccase concentration of 1 g/L. Under the above condition, the o-phenylenediamine could be removed completely after 60 min.


The results show that the removal of aniline compounds by laccase from white-rot fungi has good effect and potential application prospect.


Aniline Laccase o-phenylenediamine Removal Wastewater 



Thanks to China’s national key research and development program “2016YFC0208100”.

Authors’ contributions

Bo Yang put forward the idea and participated in the whole experiments. Removal studies for aniline compounds were performed by Yacheng Wang and Zhiguo Liu. Jun Liu wrote of the part of manuscript. M Jiaming Cai involved in purchasing of required materials and instruments, designing of removal experiments, analyzing of data and reviewing of the manuscript. All authors read and approved the final manuscript.

Compliance with ethical standards

Competing interests

The authors declare that they have no competing interests.


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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Bo Yang
    • 1
    Email author
  • Yacheng Wang
    • 1
  • Zhiguo Liu
    • 1
  • Jun Liu
    • 1
  • Jiaming Cai
    • 1
  1. 1.College of Environment and Safety EngineeringQingdao University of Science & TechnologyQingdaoChina

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