Advertisement

Effect of ethanolic preparations of cinnamon (Cinnamomum zeylanicum) extract on hematologic and histometric parameters of selected organs in Alloxan® induced diabetic female albino rats

  • Anas Sarwar QureshiEmail author
  • Junaid Ghaffor
  • Muhammad Usman
  • Nazia Ehsan
  • Zaima Umar
  • Adeel Sarfraz
Research article
  • 14 Downloads

Abstract

Purpose

Assessment of the antidiabetic effect of cinnamon bark extract in histologic damages and some hematologic parameters in Alloxan® induced diabetic female albino rats.

Method

Thirty female albino rats weighing 150–230 g were divided into five groups (n = 6): normal (G1) and diabetic groups (intraperitoneally Alloxan®-injected) including diabetic control (G2), Getformin @ 0.25 (G3), CE @ 0.10 (G4), and CE @ 0.20 g/kg b.wt. (G5) for 49 days. Blood glucose level and weight were measured on weekly interval for the period of seven weeks (49th day). Blood samples were collected for hematologic analysis. Tissue samples from uterus, liver and kidneys were processed by routine paraffine technique. Histologic sections of uterus were studied to measure endometrial glands area and thickness of endo- and myometrium. Liver and kidneys were evaluated for diabetes-induced degenerative changes and antidiabetic effect of cinnamon extract (CE). One-way analysis of variance followed by Tukey test were used to compare the group means for each parameter.

Results

Statistical analysis revealed significant (P < 0.05) deleterious effects of diabetes on all parameters studied, however, CE recovered hematological parameters significantly (P < 0.05) as seen in G3 and G5 groups which showed significant (P < 0.05) improvement in uterus, liver and kidneys’ histology. G4 significantly (P < 0.05) reduced the blood glucose at the 4th week which was maintained in subsequent weeks while G3 and G5 had significantly (P < 0.05) lowered the blood glucose from 1st week, although highly significant (P < 0.01) effect was observed during last two weeks of the study.

Conclusion

Anti-diabetic activity of cinnamon extract was found significant in Alloxan® induced hyperglycemic rats in dose-dependent manners. CE has potential to restore diabetes induced hematological disturbances and histological damages in uterus, liver and kidney due to the presence of cinnamic acid, anhydride tannin and methyl-hydroxy chalcone polymer. Hence, CE can be recommended for the management of glucose homeostasis to avoid diabetes-associated disturbances in female rats.

Keywords

Diabetes Cinnamon Internal organs Hematology Histology 

Notes

Authors contribution

AS Qureshi, M Usman and N Ehsan conceived the idea and finalized manuscript: AS Qureshi and M Usman supervised the lab work. J Ghaffor, A Sarfraz and Z Umar conducted lab work, applied statistics and prepared rough draft.

Compliance with ethical standards

Conflict of interest

Authors have no conflict of interest to declare.

Ethical approval

All procedures performed in this study involving rats were in accordance with the research ethical standards of the University of Agriculture, Faisalabad, Pakistan.

References

  1. 1.
    Lambert AP, Gillespie KM, Thomson G, Cordell HJ, Todd JA, Gale EA, et al. Absolute risk of childhood-onset type 1 diabetes defined by human leukocyte antigen class II genotype: a population-based study in the United Kingdom. J Clin Endocrinol Metab. 2004;89(8):4037–43.CrossRefGoogle Scholar
  2. 2.
    DeFronzo RA, Tripathy D. Skeletal muscle insulin resistance is the primary defect in type 2 diabetes. Diabetes Care. 2009;32(suppl 2):S157–S63.CrossRefGoogle Scholar
  3. 3.
    Usman M, Ali MZ, Qureshi A, Ateeq MK, Nisa FU. Short term effect of dose-dependent camel milk in Alloxan induced diabetes in female albino rats. J Anim Plant Sci. 2018;28(5):1292-1300.Google Scholar
  4. 4.
    Khan AA, Alzohairy MA, Mohieldein AH. Antidiabetic effects of camel milk in streptozotocin-induced diabetic rats. Am J Biochem Mol Biol. 2013;3(1):151–8.CrossRefGoogle Scholar
  5. 5.
    Baragob AEA. Composition and hypoglycemic effect of camel milk in streptozotocin-induced diabetic rats. Biochem Biotechnol Res. 2015;3:38–42.Google Scholar
  6. 6.
    Codner E, Merino P, Tena-Sempere M. Female reproduction and type 1 diabetes: from mechanisms to clinical findings. Hum Reprod Update. 2012;18(5):568–85.CrossRefGoogle Scholar
  7. 7.
    Favaro RR, Salgado RM, Raspantini PR, Fortes ZB, Zorn TM. Effects of long-term diabetes on the structure and cell proliferation of the myometrium in the early pregnancy of mice. Int J Exp Pathol. 2010;91(5):426–35.CrossRefGoogle Scholar
  8. 8.
    Bailey CJ, Day C. Traditional plant medicines as treatments for diabetes. Diabetes Care. 1989;12(8):553–64.CrossRefGoogle Scholar
  9. 9.
    Okigbo R, Putheti R, Achusi C. Post-harvest deterioration of cassava and its control using extracts of Azadirachta indica and Aframomum melegueta. E-J Chem. 2009;6(4):1274–80.CrossRefGoogle Scholar
  10. 10.
    Gruenwald J, Freder J, Armbruester N. Cinnamon and health. Crit Rev Food Sci Nutr. 2010;50(9):822–34.CrossRefGoogle Scholar
  11. 11.
    Kirkham S, Akilen R, Sharma S, Tsiami A. The potential of cinnamon to reduce blood glucose levels in patients with type 2 diabetes and insulin resistance. Diabetes Obes Metab. 2009;11(12):1100–13.CrossRefGoogle Scholar
  12. 12.
    Jarvill-Taylor KJ, Anderson RA, Graves DJ. A hydroxychalcone derived from cinnamon functions as a mimetic for insulin in 3T3-L1 adipocytes. J Am Coll Nutr. 2001;20(4):327–36.CrossRefGoogle Scholar
  13. 13.
    Dou L, Yahong Z, Li L, Gui X, Chen Y, et al. The effect of cinnamon on polycystic ovary syndrome in a mouse model. Reprod Biol Endocrinol. 2018;6:99–10.  https://doi.org/10.1186/s12958-018-0418-y.CrossRefGoogle Scholar
  14. 14.
    Oyedemi S, Yakubu M, Afolayan A. Effect of aqueous extract of Leonotis leonurus (L.) R. Br. Leaves in male Wistar rats. Hum Exp Toxicol. 2010;29(5):377–84.CrossRefGoogle Scholar
  15. 15.
    Erukainure OL, Ebuehi OA, Adeboyejo FO, Aliyu M, Elemo GN. Hematological and biochemical changes in diabetic rats fed with fiber-enriched cake. J Acute Med. 2013;3(2):39–44.CrossRefGoogle Scholar
  16. 16.
    Oyedemi S, Yakubu M, Afolayan A. Antidiabetic activities of aqueous leaves extract of Leonotis leonurus in streptozotocin induced diabetic rats. J Med Plant Res. 2011;5(1):119–25.Google Scholar
  17. 17.
    Ateeq MK, Qureshi AS, Usman M, Shahid RU, Khamas WA. Effect of Orally Administered Camel Milk in Alloxan® Induced Albino Rats: Long Term Study on Maternal Uterus and Neonates Selected Organs. Pak Vet J. 2019;39(1):81-85.Google Scholar
  18. 18.
    Rao PV, Gan SW. Cinnamon: a multifaceted medicinal plant. Evidence Based Complmen Alternat Med. 2014.  https://doi.org/10.1155/2014/642942.CrossRefGoogle Scholar
  19. 19.
    Mousa NK, Sabbar SS, Al-Karime ASA, Ahmed IA. Complete Blood Count and Cinnamic acid activity against Cytoxan in albino mice. Baghdad Sci J. 2014;11(2):1274-1280.Google Scholar
  20. 20.
    Tariq S, Nurulain SM, Rashed H, Lotfy M, Emerald SB, Koturan S, et al. Diabetes-induced changes in the morphology and nociceptinergic innervation of the rat uterus. J Mol Histol. 2016;47(1):21–33.CrossRefGoogle Scholar
  21. 21.
    Hou Z-M, Sun Q, Liu Y-Z, Chen T-F, Tang N. Effects of insulin resistance on myometrial growth. Int J Clin Exp Med. 2015;8(1):1552–7.PubMedPubMedCentralGoogle Scholar
  22. 22.
    Ali MZ, Qureshi AS, Usman M, Kausar R, Ateeq MK. Comparative effect of camel milk and black seed oil in induced diabetic female albino rats. Pak Vet J. 2017;37:293–8.Google Scholar
  23. 23.
    Ragavan B, Krishnakumari S. Effect of T. arjuna stem bark extract on histopathology of liver, kidney and pancreas of alloxan-induced diabetic rats. Afr J Biomed Res. 2006;9(3):189–197.Google Scholar
  24. 24.
    Szkudelski T. The mechanism of alloxan and streptozotocin action in B cells of the rat pancreas. Physiol Res. 2001;50(6):537–46.PubMedGoogle Scholar
  25. 25.
    Misra M, Aiman U. Alloxan: an unpredictable drug for diabetes induction? Indian J Pharm. 2012;44(4):538–9.CrossRefGoogle Scholar
  26. 26.
    Anderson RA, Broadhurst CL, Polansky MM, Schmidt WF, Khan A, Flanagan VP, et al. Isolation and characterization of polyphenol type-a polymers from cinnamon with insulin-like biological activity. J Agric Food Chem. 2004;52(1):65–70.CrossRefGoogle Scholar
  27. 27.
    Cheng D, Liang B, Li Y. Antihyperglycemic effect of Ginkgo biloba extract in streptozotocin-induced diabetes in rats. Biomed Res Int. 2012;2013.Google Scholar
  28. 28.
    Adisakwattana S. Cinnamic acid and its derivatives: mechanisms for prevention and management of diabetes and its complications. Nutrients. 2017;9(2):163.CrossRefGoogle Scholar

Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Anas Sarwar Qureshi
    • 1
    Email author
  • Junaid Ghaffor
    • 2
  • Muhammad Usman
    • 1
  • Nazia Ehsan
    • 2
  • Zaima Umar
    • 1
  • Adeel Sarfraz
    • 3
  1. 1.Department of AnatomyUniversity of AgricultureFaisalabadPakistan
  2. 2.Department of Zoology, Wildlife and Fisheries, Faculty of SciencesUniversity of AgricultureFaisalabadPakistan
  3. 3.University College of Veterinary and Animal SciencesThe Islamia University of BahawalpurBahawalpurPakistan

Personalised recommendations