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Efficacy of melatonin in restoring the antioxidant status in the lens of diabetic rats induced by streptozotocin

  • Marjan Khorsand
  • Masoumeh AkmaliEmail author
  • Morteza Akhzari
Research article
  • 1 Downloads

Abstract

Background

Melatonin is a well-known free radical scavenger. The present study aimed to investigate the effects of melatonin treatment on the antioxidant status in the lenticular tissue of streptozotocin (STZ)-induced diabetic rats.

Methods

Thirty-four male rats were randomly divided into four groups as follows: healthy control rats (group 1, n = 10); diabetic control rats (group 2, n = 10); melatonin-treated (5 mg/kg·day) diabetic rats (group 3, n = 10) and melatonin-treated (5 mg/kg·day) healthy rats (group 4, n = 4). Diabetes was induced by injection of streptozotocin (50 mg/kg, ip). Following 8-weeks of melatonin treatment, all rats were killed and the blood plasma and their lenses were stored at −70 °C for antioxidant enzyme activities assay and biochemical determination.

Results

The plasma glucose and lens malondialdehyde (MDA) increased significantly in the rats of group 2 as compared to the group 1. Also, a significant decrease in the levels of catalase (CAT) and glutathione reductase (GR) activities in the lenses and plasma reduced glutathione (GSH) was found. However, the levels of lenticular MDA (not significant) and the plasma glucose significantly decreased in the rats of group 3 compared to the group 2. Besides, the levels of CAT, GR in the rats lens and plasma GSH increased significantly.

Conclusion

Diabetes mellitus induced hyperglycemia and oxidative stress, whereas melatonin decreased the blood glucose levels and lipid peroxidation and increased the activities of antioxidant enzymes in diabetic rat lenses.

Keywords

Antioxidant enzymes Diabetes Melatonin Oxidative stress Streptozotocin 

Abbreviations

ROS

Reactive oxygen species

UVB

Ultraviolet-B

NADPH

β-Nicotinamide adenine dinucleotide phosphate-reduced form

TBA

2-thiobarbituric acid

TEP

1, 1, 3, 3-tetra ethoxy propane

GSH

Reduced glutathione

DTNB

5, 5′-dithiobis-(2-nitrobenzoic acid)

GSSG

Oxidized glutathione

STZ

Streptozotocin

MDA

Malondialdehyde

GR

Glutathione reductase

NADPH

Reduced nicotinamide adenine dinucleotide phosphate

CAT

Catalase

GSH

Reduced glutathione

SOD

Superoxide dismutase

GSH-Px

Glutathione peroxidase

RNS

Reactive nitrogen species

EDTA

Ethylenediaminetetraacetic acid

BSA

Bovine serum albumin, Non-insulin-dependent diabetes mellitus (NIDDM)

Notes

Acknowledgements

The authors thank from the biochemistry department of Shiraz University of Medical Sciences for technical assistance in this work.

Authors’ contributions

Masoumeh Akmali contributed to the design the study, and drafted the manuscript, Masoumeh Akmali, Marjan Khorsand and Morteza Akhzari conducted the analysis, wrote and revised manuscript, Marjan Khorsand performed the biochemical analysis and animal treatment.

Funding

The Shahid Sadoughi University of Medical Sciences (Yazd, Iran) and Shiraz University of Medical Sciences (Shiraz, Iran) funded this study, which is derived from the student thesis of Marjan Khorsand by grant number 1083.

Compliance with ethical standards

Ethics approval and consent to participate

All experiments in this study performed according to the guidelines of “Animal Care Ethics Committee” of Shiraz University of Medical Sciences, Shiraz, Iran (IR.SUMS.REC).

Conflict of interest

There is no financial or personal conflict of interest.

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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Marjan Khorsand
    • 1
    • 2
  • Masoumeh Akmali
    • 2
    Email author
  • Morteza Akhzari
    • 2
  1. 1.Department of Biochemistry and Molecular BiologyShahid Sadoughi University of Medical SciencesYazdIran
  2. 2.Department of BiochemistryShiraz University of Medical Sciences, Medical SchoolShirazIran

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