Microwave assisted synthesis of polyacrylamide grafted polymeric blend of fenugreek seed mucilage-Polyvinyl alcohol (FSM-PVA-g-PAM) and its characterizations as tissue engineered scaffold and as a drug delivery device
- 12 Downloads
Microwave assisted synthesis of graft copolymer of polymeric blend of Fenugreek seed mucilage (FSM)-Polyvinyl alcohol (PVA) with acrylamide (AM) was done by free radical polymerization using ammonium per sulfate (APS) as initiator. Varying amount of AM and APS was used to optimize the best grade based on highest percentage grafting efficiency and investigated with intrinsic viscosity measurement, Fourier Transformation infrared spectroscopy (FTIR),13C NMR spectra, X-ray diffraction, elemental analysis, Thermogravimetric analysis, Scanning electron microscopy. The results of intrinsic viscosity indicate that the optimized sample GF4 has longer chain length than in comparison to the native mucilage and thus exhibits more swelling tendencies and thus can be used as very good controlled release matrix system. The thermal analysis and X-ray indicates that GF4 is more stable and possess more amorphous properties than the native FSM. The NMR and FT-IR studies reveal that in GF4 there is prominent presence of amide and the hydroxyl groups indicating that grafting mechanism has efficiently taken place. Histological studies & SEM image for optimized grade implanted on animals revealed sufficient tissue growth and exhibited biodegradability proving the material to be biocompatible and suitable to be used as tissue engineered scaffolds. The controlled release behavior of the optimized polymeric system GF4 was evidenced by 95% release of loaded drug Enalapril maleate for 16 h.
KeywordsGraft copolymer Metronidazole Histopathology Scaffold Fenugreek seed mucilage
The authors acknowledge the instrumental support of Central Instrumentation Facility, Birla Institute of Technology, Mesra, for sophisticated instrument to carry out this experiment.
The experimental study was done by Corresponding author Trishna Bal (TB) as a part of her reserach work who did all the practical experiments and drafted the article. The other coauthor, Sabyasachi Swain (SS) did the insertion of the polymeric scaffolds in animals. All authors read and approved the final copy of the text.
Compliance with ethical standards
Conflict of interest
The authors declare that there is no conflict of interest.
- 4.Maiti S, Ranjit S, Sa B. Polysaccharide-based graft copolymers in controlled drug delivery. Int J PharmTech Res. 2010;2:1350–8.Google Scholar
- 16.Sharma RK, Lalita, Singh AP. Synthesis and characterization of chitosan based graft copolymers for drug release applications. J Chem Pharm Res. 2015;7(6):612–21.Google Scholar
- 18.Schott H. Polymers. In: Martin A, Bustamante P, Chun AHC, editors. Physical pharmacy. 4th ed. Maryland: B.I.Waverly Pvt. Ltd; 1994. p. 561–3.Google Scholar
- 25.Nishida E, Miyaji H, Kato A, HirokoTakita TI, TakehitoMomose KO, Murakami S, et al. Graphene oxide scaffold accelerates cellular proliferative response and alveolar bone healing of tooth extraction socket. Int J Nanomedicine. 2016;11:2265–77.Google Scholar
- 26.Beauchamp. Infrared Tables (short summary of common absorption frequencies) https://www.cpp.edu/~psbeauchamp/pdf/spec_ir_nmr_spectra_tables.pdf. Accessed 5 Oct 2018.
- 28.Songa Y, Wei D. Preparation and characterization of graft copolymers of silk Sericin and methyl methacrylate. Polym Polym Compos. 2006;14(2):169–74.Google Scholar
- 33.Swain S, Bal T. Microwave irradiated carrageenan-guar gum micro-porous IPN: a novel material for isotropic tissue scaffolding. Int J Polym Mater Polym Biomater. 2018:1–9. https://doi.org/10.1080/00914037.2018.1506986.