The Claim of Anti-Cataract Potential of Heliotropium indicum: A Myth or Reality?
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Heliotropium indicum has several uses in traditional medicine attributable to its numerous bioactive compounds. It is used as a traditional remedy for cataracts in Ghana without any scientific verification. This study aimed at verifying the anti-cataract properties of an aqueous whole plant extract of H. indicum.
The effect (cataract score) of 30, 100, and 300 mg kg−1 extract (bid for 21 days, per os) on the development of 30 µmol kg−1 sodium selenite-induced cataract in 10-day-old rat pups was investigated. Soluble lens proteins alpha A and alpha B crystallins, total lens protein, total lens glutathione, and aquaporin 0 in enucleated lens homogenates were determined spectrophotometrically using commercially available kits. Histopathological studies on the lenses were also performed. The 2,2-diphenyl-1-picrylhydrazyl scavenging effect and linoleic acid autoxidation (antioxidant properties) of the extract (0.1–3.0 mg ml−1), compared to n-propyl gallate, were ascertained using standard procedures.
Cataract scores showed that the extract, at all dose levels, significantly alleviated selenite-induced cataracts (P ≤ 0.001). Markers of lens transparency (aquaporin 0, alpha A and B crystallins), as well as total lens proteins and lens glutathione levels, were significantly preserved (P ≤ 0.01–0.001). The extract exhibited activity relevant for scavenging free radicals and inhibition of lipid peroxidation. Epithelial and lens fiber integrity in the histopathological assessment were maintained with HIE treatment.
The aqueous whole plant extract of H. indicum significantly inhibited the development of cataracts in rats via multiple mechanisms.
KeywordsAlpha A and alpha B crystallins Aquaporin 0 Cataract DPPH scavenging effect
Heliotropium indicum (Family: Boraginaceae) has long been used in traditional medicine for the treatment of several ailments not only in Ghana but also in several other parts of the globe . Its medicinal prowess has led to several scientific investigations into its pharmacological activity and the isolation of several alkaloids of pharmacological importance. Notable among these alkaloids are indicine, indicine-N-oxide, acetyl-indicine, indicinine, heleurine, heliotrine, supinine, supinidine, and lindelofidine [2, 3, 4, 5, 6, 7]. Some of these bioactive compounds, such as indicine-N-oxide, have gained attention for their anti-cancer properties despite safety concerns .
With the advances made in investigating H. indicum’s bioactivity in managing various systems disorders, its traditional uses as an antidote for ocular disorders such as conjunctivitis, uveitis, and cataract remain largely unverified [8, 9].
Against the backdrop that cataracts remain the leading cause of blindness in the world, and the number one cause of ocular morbidity among the elderly with limited management options (only surgery is approved), claims and unverified reports of potential anti-cataract agents deserve attention [10, 11]. This search for alternative treatment for chronic disorders such as cataracts has rejuvenated scientists’ interest in plant medicine as a possible source of remedy, some of which have made their way into mainstream allopathic medicine. Examples of these plant-derived medications include atropine, muscarine, and physostigmine . However, amidst all these developments, plant-based pharmaceuticals are still largely untapped. Of the estimated 250,000–500,000 plant species, only a small percentage have been investigated phytochemically and an even smaller percentage have been well studied regarding their pharmacological action . This highlights the need for rigorous scientific research into the botanicals used in traditional medicine as a basis for developing novel medication to cater for unmet medical needs.
Based on this assertion, this study sought to conduct an initial investigation aimed at examining the anti-cataract claims of H. indicum commonly used either orally (whole plant) or topically (leaves) by some Ghanaian people to ascertain the veracity of this claim.
Heliotropium indicum was collected from the botanical gardens of the University of Cape Coast (5.1036° N, 1.2825° W), Cape Coast, in the Central Region of Ghana, in November 2012. It was identified and authenticated by a botanist at the University of Cape Coast herbarium, where a voucher specimen bearing the number 4873 has been deposited for future reference.
Preparation of the Aqueous Whole Plant Extract of H. indicum
Whole plants of H. indicum were washed thoroughly with tap water and shade dried. The dry plants were milled into a coarse powder by a hammer mill (Schutte Buffalo, New York, NY, USA). One and a half kilograms of the plant powder was mixed with one liter of water. The mixture was Soxhlet extracted at 80 °C for 24 h. The aqueous extract obtained was freeze-dried (Hull freeze-dryer/lyophilizer 140 SQ, Warminster, PA, USA). The powder (yield 12.2%), labeled as whole plant extract of H. indicum (HIE), was stored at 4 °C and reconstituted in normal saline to the desired concentration for dosing in this study.
Drugs and Chemicals
Sodium selenite, Na2SeO3 (Sigma Aldrich, Germany), was used to induce cataracts. Tropicamide 1% ophthalmic solution [Alcon Laboratories South Africa (Pty) Ltd] was used to dilate the pupil of the eye at every slit-lamp examination. 2,2-Diphenyl-1-picrylhydrazyl(DPPH), methanol, ethanol, sodium phosphate, n-propyl gallate, linoleic acid (BDH, Poole, UK), trichloroacetic acid, and thiobarbituric acid (Sigma-Aldrich, St. Louis, MO, USA) were used in the antioxidant assays.
Animals and Husbandry
Ten-day-old Sprague–Dawley rat pups of either sex (mean weight of 24 ± 2 g) were kept in the Animal House of the School of Biological Sciences, University of Cape Coast. The pups (and their mothers) were housed in polyacrylic cages (34 × 47 × 18 cm) with soft wood shavings as bedding, under ambient laboratory conditions (temperature 28 ± 2 °C, relative humidity 60–70%, and a normal light–dark cycle). The mothers were fed on a normal commercial pellet diet (Agricare Ltd., Kumasi, Ghana) and had access to water ad libitum.
Ethical and Biosafety Considerations
The study protocol was approved by the Institutional Review Board on Animal Experimentation, Faculty of Pharmacy and Pharmaceutical Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana (Ethical Clearance Number: FPPS/PCOL/0030/2013). All activities performed during the studies conformed to accepted principles for laboratory animal use and care (EU directive of 1986: 86/609/EEC) and Association for Research in Vision and Ophthalmology Statement for Use of Animals in Ophthalmic and Vision research. Biosafety guidelines for the protection of personnel in the laboratory were observed.
Preliminary Phytochemical Screening
Effect of HIE on Selenite-Induction of Cataract in Pups
Ten-day-old rat pups were injected subcutaneously with 15 µmol kg−1 sodium selenite in normal saline daily for 2 days. The pups were then put into four groups (n = 8, i.e., the number of rats per group). Groups I–IV received 30, 100, 300 mg kg−1 HIE, and 10 mL kg−1 normal saline, respectively, per os, 30 min after the first selenite injection. These doses were used based on preliminary studies to establish activity in our laboratory. Treatment was then given twice daily (12 h) for 21 days. A fifth group (V) made up of normal pups given no selenite injection nor treatment was also kept under same experimental conditions as a normal control. On day 21 after selenite injection, the pupils of the pups’ eyes were dilated using 1% tropicamide ophthalmic solution and assessment of the crystalline lens for developing cataract made using a Marco II-B Slit Lamp (Marco-Lombart Instrument, Japan). The developing cataracts were then graded and scored after repeating the experiment on three different occasions.
Grading and Scoring of Cataract
Grading and scoring of selenite-induced cataract in lenses of Sprague–Dawley rats
Swollen lens fibers and subcapsular opacities
Nuclear cataract was observed in the lens but swollen fibers were still visible in the lens cortex
Strong nuclear cataract with peri-nuclear area opacity in the lens
Total opacity of the lens
Determination of Lens Glutathione
The total glutathione (GSH) in the crystalline lens was determined using a commercially acquired kit (Cayman Chemicals, Ann Arbor, MI, USA). The lenses from the enucleated eye of the rats were rinsed with phosphate-buffered saline (PBS), pH 7.4, to remove any blood clots. They were then homogenized in 5 mL of 50 mM MES (2-(N-morpholino) ethanesulfonic acid, pH 7), using Precellys 24 tissue homogenizer (Bertin Technologies, France). The lens homogenate was centrifuged at 100,000g for 15 min at a temperature of 4 °C, after which the supernatant was deproteinated using metaphosphoric acid and 4 M triethanolamine according to the manufacturer’s instructions. A 50 µL volume of the deproteinated lens samples and the standards (constituted per the manufacturer’s directive) were pipetted into a 96-well microtiter plate and read at 405 nm with a URIT-660 microplate reader (URIT Medical Electronic Co., Ltd, Guangxi, China) after incubation in the dark on an orbital shaker for 30 min. Each determination was done in triplicate.
Determination of Total Lens Protein Concentration
A bicinchoninic acid (BCA) protein assay kit (Pierce, Rockford, IL, USA) was used to establish the total protein concentration in the lens homogenate obtained from the enucleated rat eyes. For total protein estimation, the lens homogenate was prepared in 5% trichloroacetic acid. The precipitated protein was dissolved in 5% sodium hydroxide and used as aliquots for the estimation of total proteins. The protein concentration was determined by pipetting 50 μL of lens homogenate from: rats treated with 30, 100, and 300 mg kg−1 of HIE, and 10 mL kg−1 normal saline; normal rats without selenite injection; and standard bovine serum albumin (BSA) into a 96-well microplate. A 200 μL volume of working reagent, constituted according to the manufacturer’s instructions, was mixed thoroughly with the content of each well and shaken for 30 s. It was then incubated at 37 °C for 30 min and allowed to cool to room temperature. Absorbances of the mixtures were measured at 562 nm using an URIT-660 microplate reader. Each determination was done in triplicate.
Determination of Soluble Lens Protein
Soluble lens proteins were determined using a commercially available ELISA kits (MyBioSource, Inc., San Diego, CA, USA). Soluble lens protein levels, mainly alpha A (CRYAA) and alpha B (CRYAB), were estimated after the lens tissue was rinsed with PBS, homogenized in 1 mL of 1X PBS, and stored overnight at −20 °C. After two freeze–thaw cycles were performed to break the cell membranes, the homogenates were centrifuged for 5 min at 5000g and 2–8 °C. The supernatant was removed and assayed immediately. A 100 μL of standard and sample per well was constituted, covered with an adhesive strip, and incubated for 2 h at 37 °C. Several other intermittent incubations were done in accordance with the manufacturer’s instructions. Absorbances of the mixtures were measured at 450 nm using a URIT-660 microplate reader. Each determination was done in duplicate.
Determination of Aquaporin 0
A commercial kit which uses a double-antibody sandwich enzyme-linked immunosorbent one-step process assay (ELISA; MyBioSource, Inc., MBS 028997) was used to assay the level of aquaporin 0 (AQP0) in lens homogenate samples. Standard, test sample, and HRP-labeled AQP0 antibodies were pipetted into the enzyme wells which are pre-coated with AQP0 antibody. These were then incubated (at 37 °C in the dark at different time periods as specified by the manufacturer) and washed manually with de-ionized water using a Pasteur pipette to remove the uncombined enzyme. Upon adding Chromogen Solution A and B, the color of the liquid changed to blue and finally to yellow after adding the stop solution. The optical density (OD) was then measured at 450 nm using a URIT-660 microplate reader. Based on the concentration standards and their corresponding OD values, a standard curve linear regression equation was computed. From this curve, the OD values of the sample on the regression equation were used to calculate the corresponding sample’s concentration.
The extracted lenses from enucleated eyes of the animals were fixed in 10% phosphate-buffered paraformaldehyde and embedded in paraffin. Sections were made and stained with hematoxylin and eosin , and fixed on glass slides for microscopic examination at the Department of Biomedical and Forensic science, University of Cape Coast, Cape Coast, for histopathological assessment by an experimental pathologist.
Antioxidant Property of HIE
Free Radical Scavenging Assay
This procedure was performed as previously described by Govindarajan et al.  with few modifications. Concentrations of 0.1–3.0 mg mL−1 HIE in 99.8% methanol were selected and compared with 0.001–0.03 mg mL−1 n-propyl gallate in methanol (reference free radical scavenger). HIE was centrifuged at 3000g for 10 min. One milliliter of the supernatant was added to 3 mL of a 20 mg L−1 methanolic DPPH solution and kept at 25 °C for 1 h in an orbital shaker (BoroLabs, Aldermaston, Berkshire, UK). A 1 mL quantity of 99.8% methanol (control) was added to 3 mL DPPH solution and incubated under the same conditions. The absorbance of the residual DPPH was determined at 517 nm in a Cecil CE 2040 spectrophotometer (Cecil Instruments Limited, Cambridge, UK). Methanol was used as the blank. The procedure was done in triplicate and the results expressed as percentage DPPH scavenging effect against concentration. The half maximal inhibitory concentration (IC50) values were established.
Linoleic Acid Autoxidation
Statistical analysis of the data obtained was done using GraphPad Prism version 5.0 (GraphPad Software, Inc., La Jolla, CA, USA). Differences between treatment groups and the controls were estimated using one-way analysis of variance (ANOVA) followed by Dunnet’s multiple comparisons test (post hoc test) at a confidence level of 95%. Probability values ≤5% (P ≤ 0.05) were considered significant.
Results obtained after preliminary phytochemical screening of HIE
Effect of HIE on Selenite-Induced Cataracts
Effect of HIE on Lens GSH
Total Lens Protein and Soluble Protein Concentration
Total lens protein and soluble protein levels of controls and HIE-treated cataractous Sprague–Dawley rats
Total protein (µg mL−1)
CRYAA (pg mL−1)
CRYAB (pg mL−1)
10 mL kg−1 NS
2135 ± 158.0
167.3 ± 13.14
84.30 ± 3.303
4369 ± 108.1***
673.2 ± 43.09***
153.7 ± 3.303***
30 mg kg−1 HIE
3500 ± 39.67***
515.5 ± 34.77***
153.7 ± 3.303***
100 mg kg−1 HIE
2915 ± 167.0**
331.6 ± 22.76**
117.3 ± 8.739**
300 mg kg1 HIE
3545 ± 83.48***
719.2 ± 6.571***
160.3 ± 5.721***
Aquaporin 0 Levels in the Lens
Free Radical Scavenging Effect and Linoleic Acid Autoxidation Assay
Selenite-induced cataract is a common model of senile cataract employed in preclinical studies for screening potential anti-cataract agents . Overdose of this salt has been hypothesized to cause cataracts by a series of processes which includes: alteration of epithelial cell metabolism , enhanced oxidative stress , impairment of calcium homeostasis [22, 23, 24], apoptosis [25, 26], DNA damage , activation of m-calpain , and insolubilization of proteolyzed β- and α-crystallins .
Although the 100 mg kg−1 HIE dose showed a slight drop in activity relative to the 30 and 300 mg kg−1 doses, its observed clinical effect is desirable since a delay in cataract formation is known to prevent severe visual disability that could affect the independence of most people affected . It has been projected that a delay in cataract formation of approximately 10 years would lessen the burden of a visually disabling cataract by approximately 45% . Such a delay, as shown by 100 mg kg−1 HIE, has the potential to enhance the quality of life for most of the world’s elderly population at risk of cataract, and to considerably moderate both the economic burden due to disability and surgery related to cataract after further studies regarding isolation, structural elucidation, and efficacy have been performed.
Safeguarding the lens from oxidative stress, and the consequent maintenance of transparency, is ensured by endogenous antioxidant systems that consist of non-enzymatic and enzymatic machinery. Human senile or experimental cataractogenesis is linked with a progressive decline in lenticular GSH [31, 32]. For that reason, it has been hypothesized that GSH plays a key role in maintaining lens physiology and transparency by protecting sulfhydryl groups from oxidation [33, 34]. The acknowledged impact of oxidative damage in the pathology of cataracts, and in the aging process, has shifted attention to antioxidant therapy. Flavonoids and tannin in general have been proven to possess antioxidant activity relevant for scavenging free radicals and to inhibit lipid peroxidation [35, 36, 37].
The series of biochemical alterations that take place in selenite-induced cataractogenesis include the reduction of total soluble proteins of lens and loss of total protein concentration . This presupposes that selenite-induced cataractogenesis affects the whole lens transport system. Selenite has again been reported to inhibit protein synthesis in test tube and human subjects after repeated exposures [39, 40]. The conservation of the total protein concentration in the lens of the extract treated indicates that it is exerts it anti-cataract effect by preventing the inhibition of protein synthesis in the lens. Moreover, selenium salt (i.e., sodium selenite) causes a loss of α-crystallins (a major protein component of the vertebrate eye lens) which have been found to exert a chaperone activity by binding to unfolded or denatured proteins and suppressing nonspecific aggregation . This chaperone role helps to avert the development of large light-scattering aggregates, inactivation of oxidative enzymes, and probable cataractogenesis [42, 43]. The extract treatment preserved the α-crystallin A and B in the right molar concentration of three to one . This high concentration of total lens protein and water-soluble proteins in their right proportion is necessary for the maintenance of the optimum refractive index .
Gradual but persistent deterioration of lens tissue occurs as a pathophysiological consequence of malfunctioning water and nutrient exchange via channel and transporter proteins. These transporter proteins are crucial because of the avascular nature of the crystalline lens which may not survive on diffusion alone . AQP0, also known as major intrinsic polypeptide (MIP), constitutes more than 60% of the total membrane protein content of fiber cells . Aquaporins form channels for water permeability across biological membranes by high caliber 6-transmembrane helices that bring together a hydrophilic water-conducting pore . AQP0 serves as a model to appreciate how water channels are regulated at the molecular level . The preservation of the AQP0 content of the lens via the extract treatment contributed to the maintenance of the lens transparency.
The possible mechanism of the anti-cataract effect of HIE is not definitive as there are multiple bioactive ingredients which may have either synergistic or antagonist effects depending on the dose. This possible interaction is predictive from the relative drop in effect of the 100 mg kg−1 to the other doses and deserves further investigation.
The aqueous extract of H. indicum has anti-cataract activity on selenite-induced cataracts in Sprague–Dawley rats and maybe useful as an anti-cataract agent for human cataracts after further studies.
This study was partly funded by University of Cape Coast. We appreciate the technical assistance offered by Rex Ananne and Daniels Konja of the Department of Biomedical and Forensic Sciences, School of Biological Sciences, University of Cape Coast, Cape Coast, Ghana. This article results from research towards a Ph.D. (Optometry) degree in the Discipline of Optometry at the University of KwaZulu-Natal under the supervision of Dr. George A. Koffuor and co- supervision of Prof. Paul Ramkissoon. All named authors meet the International Committee of Medical Journal Editors (ICMJE) criteria for authorship for this manuscript, take responsibility for the integrity of the work as a whole, and have given final approval for the version to be published.
Samuel Kyei, George Asumeng Koffuor, Paul Ramkissoon, Clement Afari, and Emmanuel Akomanin Asiamah declare that they have no conflict of interest.
Compliance with ethics guidelines
The study protocol was approved by the Institutional Review Board on Animal Experimentation, Faculty of Pharmacy and Pharmaceutical Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana (Ethical clearance number: FPPS/PCOL/0030/2013). All activities performed during the studies conformed to accepted principles for laboratory animal use and care (EU directive of 1986: 86/609/EEC) and Association for Research in Vision and Ophthalmology Statement for Use of Animals in Ophthalmic and Vision research. Biosafety guidelines for the protection of personnel in the laboratory were observed.
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- 1.Gurib-Fakim A. Heliotropium indicum L. In: Schmelzer GH, Gurib-Fakim A editors. Prota. 2006;11:(1) Medicinal plants/Plantes médicinales 1. [CD-Rom]. PROTA, Wageningen.Google Scholar
- 2.Pandey DP, Singh JP, Roy R, Singh VP, Pandey VB. Constituents of Heliotropium indicum. Orient J Chem. 1996;12:321–2.Google Scholar
- 3.Mattocks AR. Minor alkaloids of H. indicum. J Chem Soc. 1967;15:329–31.Google Scholar
- 4.Mattocks AR, Schoental R, Crowley HC, Culvenor CCJ. 1064. Indicine: the major alkaloid of Heliotropium indicum L. J Chem Soc. 1961;5400–5403.Google Scholar
- 5.Dutta SK, Sanyal U, Chakraborty SK. A modified method of isolation of Indicine-N-Oxide from H. indicum and its antitumor activity against ehrlich ascites carcinoma and sarcoma-180. Indian J Cancer Chem. 1987;9:73–7.Google Scholar
- 8.Rahmatullah M, Das AK, Mollik M, Jahan R, Khan M, Rahman T, et al. An ethnomedicinal survey of Dhamrai sub-district in Dhaka District, Bangladesh. Am Eurasian J Sustain Agric. 2009;3:881–8.Google Scholar
- 9.Mollik MAH, Hossan MS, Paul AK, Rahman MT-U, Jahan R, Rahmatullah M. A comparative analysis of medicinal plants used by folk medicinal healers in three districts of Bangladesh and inquiry as to mode of selection of medicinal plants. Ethnobot Res Appl. 2010;8:195–218.Google Scholar
- 11.Mariotti SP. Global data on visual impairments 2010. World Health Organization, 20 Avenue Appia, 1211 Geneva 27, Switzerland, 2012. WHO/NMH/PBD/12.01.Google Scholar
- 12.Williamson E, Okpako DT, Evans FJ. Selection, preparation and pharmacological evaluation of plant material. Chichester: Wiley; 1996.Google Scholar
- 14.Harborne JB. Phytochemical methods: a guide to modern techniques of plant analysis. 3rd ed. London: Chapman and Hall; 1998.Google Scholar
- 30.Kupfer C. The conquest of cataract: a global challenge. Trans Ophthal Soc UK. 1984;104:1–10.Google Scholar
- 37.Pragada RR, Rao Ethadi S, Yasodhara B, Praneeth Dasari VS, Mallikarjuna Rao T. In-vitro antioxidant and antibacterial activities of different fractions of Heliotropium indicum L. J Pharm Res. 2012;5(2):1051.Google Scholar
- 40.Petry JK. The effect of selenium (Se) on blood serum protein levels in occupational contact. Pregl Lek. 1970;26:552.Google Scholar
- 44.Bloemendal H. Molecular and cellular biology of the eye lens. New York: Wiley; 1981.Google Scholar