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Bubaline Aortic Matrix: Histologic, Imaging, Fourier Transform Infrared Spectroscopic Characterization and Application into Cattle Abdominal Hernia Repair

  • Shruti D. Vora
  • Vineet KumarEmail author
  • V. K. Singh
  • D. T. Fefar
  • H. P. Gajera
Research Article
  • 13 Downloads

Abstract

Extracellular matrix (ECM) scaffolds have been shown to promote constructive remodeling response into a host. Minimal disruption of ECM ultrastructure during tissue processing is typically desirable. The purpose of the study was to prepare and characterize bubaline aortic matrix (BAM) for abdominal hernia repair in cattle. The bubaline aorta was decellularized with 1% sodium dodecyl sulfate (SDS) for 24 h followed by 0.25% trypsin for 2 h and again with 1% SDS for 24 h. Native and decellularized aortae were examined for residual cells, collagen structure and integrity by histologic examination and scanning electron microscopy (SEM). Masson’s trichrome and Weigert’s staining, DNA quantification and Fourier transform infrared (FTIR) spectroscopy were used for further characterization of BAM. BAM was used for repair of abdominal hernia in six cattle having mean weight of 132.50 ± 21.86 kg and hernial ring size of 40.55 ± 15.42 cm2. Clinical, hemato-biochemical and antioxidant parameters were evaluated to assess biocompatibility of xenogenic BAM. Histologically, the absence of cells and orderly arranged collagen fibers were observed in treated aorta. SEM confirmed the preservation of collagen structure and integrity. DNA content was significantly (P < 0.001) reduced in BAM (6.47 ± 1.26 ng/mg) as compared to the native aorta (488.11 ± 49.12 ng/mg). DNA extracts from BAM show marked removal of DNA material, with the absence of DNA band in agarose gel. FTIR spectrum of BAM showed all characteristic transmittance peaks of native aorta collagen indicating preserved collagen structure within decellularized aorta. Cattle with the BAM implant recovered uneventfully and remained sound. Hemato-biochemical and antioxidant findings were unremarkable. Bubaline aortic matrix shows excellent repair efficiency and biocompatibility for abdominal hernia repair in cattle without complications.

Keywords

Biocompatibility Bubaline aortic matrix Cattle DNA quantification FTIR spectroscopy Scanning electron microscopy 

Notes

Acknowledgements

This paper includes a part of MVSc thesis of the first author approved by Junagadh Agricultural University (JAU) and was supported by the Director of Research, JAU, Gujarat (India).

Compliance with Ethical Standards

Conflict of interest

The authors declare that there is no conflict of interest to publish this manuscript.

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Copyright information

© The National Academy of Sciences, India 2019

Authors and Affiliations

  1. 1.Department of Veterinary Surgery and Radiology, College of Veterinary Science and Animal HusbandryJunagadh Agricultural UniversityGujaratIndia
  2. 2.Department of Veterinary Physiology and Biochemistry, College of Veterinary Science and Animal HusbandryJunagadh Agricultural UniversityGujaratIndia
  3. 3.Department of Veterinary Pathology, College of Veterinary Science and Animal HusbandryJunagadh Agricultural UniversityGujaratIndia
  4. 4.Department of BiotechnologyJunagadh Agricultural UniversityGujaratIndia
  5. 5.Department of Veterinary Surgery and Radiology, College of Veterinary and Animal SciencesSardar Vallabhbhai Patel University of Agriculture and TechnologyMeerutIndia

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