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Characterization, Antimicrobial and α-Amylase Inhibitory Activity of Silver Nanoparticles Synthesized by using Mushroom Extract of Lentinus tuber-regium

  • Gopal DebnathEmail author
  • Panna Das
  • Ajay Krishna Saha
Research Article
  • 19 Downloads

Abstract

The present investigation was conducted to synthesize and characterize silver nanoparticles using wild edible mushroom extract of Lentinus tuber-regium. The current investigation also aims to determine antimicrobial and α-amylase inhibitory activity of biosynthesized silver nanoparticles (AgNPs). UV–Visible spectrophotometer study indicated that the surface plasmon resonance peak of reaction of AgNPs was around at 433 nm which confirmed the formation of AgNPs. Fourier-transform infrared spectroscopy analysis confirms that carboxyl functional groups in mushroom extract are mainly responsible in reduction of Ag+ ions to Ag0 nanoparticles. Atomic force microscopy, transmission electron microscopy and scanning electron microscopy analysis revealed that synthesized AgNPs were spherical and have an average size within 5 to 35 nm. Selected area electron diffraction and X-ray diffraction pattern displayed that synthesized AgNPs were crystalline in nature. Energy-dispersive X-ray spectroscopy study showed 10.50% of silver metal in weight in the sample. Biosynthesized AgNPs showed more antibacterial activity in Gram (−) bacteria than Gram (+). Minimum inhibitory concentration values of the biosynthesized AgNPs against Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus and Bacillus subtilis were found to be 9, 12, 14 and 13 µg/ml, respectively. Inhibition of α-amylase activity increased with increasing concentration of biosynthesized AgNPs. This work substantially indicates that mushroom is efficient in biosynthesis of silver nanoparticles having potential antimicrobial and α-amylase inhibitory activity.

Keywords

Lentinus tuber-regium Silver nanoparticles α-Amylase Antimicrobial activity MIC 

Notes

Acknowledgements

The authors are grateful to the Head, Department of Botany, Tripura University, for providing facilities. The first author is grateful to UGC, Government of India, for BSR fellowship.

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest to publish this manuscript.

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Copyright information

© The National Academy of Sciences, India 2019

Authors and Affiliations

  1. 1.Mycology and Plant Pathology Laboratory, Department of BotanyTripura UniversitySuryamaninagarIndia
  2. 2.Microbiology Laboratory, Department of BotanyTripura UniversitySuryamaninagarIndia

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