Genetic Diversity, Population Structure and Correlation Study in Moringa oleifera Lam. Using ISSR and SRAP Markers

  • R. Rajalakshmi
  • S. RajalakshmiEmail author
  • Ajay Parida
Research Article


Moringa oleifera Lam. is promoted in homestead gardens and is extensively cultivated as an affordable nutrition source to combat micronutrient-related malnutrition. This study elucidates the correlation between M. oleifera Lam. leaf iron concentration with inter-simple sequence repeat (ISSR) and sequence-related amplified polymorphism (SRAP) markers. Genetic variability analysis in 97 M. oleifera Lam. accessions indicated an average of 59.6% polymorphism through ISSR and 70.13% with SRAP markers. The mean gene diversity h (0.2), gene flow Nm (2.86) and a PhiPT value (0.14) estimated for the markers indicate the high variability existent at intra-population level. Mantel test revealed a moderate positive correlation between the ISSR and SRAP markers (r = 0.53). Population structure results and cluster tree divided the samples into two groups: One group consists of Tamil Nadu accessions and the other comprises Andhra Pradesh and Odisha accessions. Association study based on multiple regression analysis and Pearson’s correlation coefficient unveiled two markers ISSR26 and SRAP4F3R exhibiting a significant (P < 0.01) and (P < 0.05) low positive correlation with leaf iron concentration.


Moringa oleifera Lam. Genetic variability ISSR SRAP Leaf iron concentration Correlation 



The authors wish to thank Pratiksha Trust for the financial support.

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest to publish this manuscript.

Supplementary material

40011_2018_1059_MOESM1_ESM.pdf (89 kb)
Supplementary material 1 (PDF 88 kb)


  1. 1.
    Ramachandran C, Peter K, Gopalakrishnan P (1980) Drumstick (Moringa oleifera): a multipurpose Indian vegetable. Econ Bot 34:276–283CrossRefGoogle Scholar
  2. 2.
    Fahey JW (2005) Moringa oleifera: a review of the medical evidence for its nutritional, therapeutic, and prophylactic properties. Part 1. Trees Life J 1:1–15Google Scholar
  3. 3.
    Kumssa DB, Joy EJ, Young SD, Odee DW, Ander EL, Broadley MR (2017) Variation in the mineral element concentration of Moringa oleifera Lam. and M. stenopetala (Bak. f.) Cuf.: role in human nutrition. PLoS ONE 12(4):e0175503CrossRefGoogle Scholar
  4. 4.
    Gopalakrishnan L, Doriya K, Kumar DS (2016) Moringa oleifera: a review on nutritive importance and its medicinal application. Food Sci Hum Wellness 5:49–56CrossRefGoogle Scholar
  5. 5.
    Saini RK, Manoj P, Shetty NP, Srinivasan K, Giridhar P (2014) Dietary iron supplements and Moringa oleifera leaves influence the liver hepcidin messenger RNA expression and biochemical indices of iron status in rats. Nutr Res 34:630–638CrossRefGoogle Scholar
  6. 6.
    Selvakumari P, Ponnuswami V (2017) Correlation and genetic variation of thirty four different genotypes of Moringa (Moringa oleifera, Lam.) in Tamil Nadu Condition, India. Int J Curr Microbiol Appl Sci 6:332–335CrossRefGoogle Scholar
  7. 7.
    Kumar Ganesan S, Singh R, Choudhury DR, Bharadwaj J, Gupta V, Singode A (2014) Genetic diversity and population structure study of drumstick (Moringa oleifera Lam.) using morphological and SSR markers. Ind Crops Prod 60:316–325CrossRefGoogle Scholar
  8. 8.
    Muluvi GM, Sprent J, Soranzo N, Provan J, Odee D, Folkard G, McNicol J, Powell W (1999) Amplified fragment length polymorphism (AFLP) analysis of genetic variation in Moringa oleifera Lam. Mol Ecol 8:463–470CrossRefGoogle Scholar
  9. 9.
    Jatin K, Veena A (2017) Analysis of genetic diversity and population genetic structure in Simarouba glauca DC. (an important bio-energy crop) employing ISSR and SRAP markers. Ind Crops Prod 100:198–207CrossRefGoogle Scholar
  10. 10.
    Li G, Quiros C (2001) Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction: its application to mapping and gene tagging in Brassica. Theor Appl Genet 103:455–461CrossRefGoogle Scholar
  11. 11.
    Wu YG, Guo QS, He JC, Lin YF, Luo LJ (2010) Genetic diversity analysis among and within populations of Pogostemon cablin from China with ISSR and SRAP markers. Biochem Syst Ecol 38:63–72CrossRefGoogle Scholar
  12. 12.
    Aneja B, Yadav NR, Yadav RC, Kumar R (2013) Sequence related amplified polymorphism (SRAP) analysis for genetic diversity and micronutrient content among gene pools in Mungbean [Vigna radiata (L.) Wilczek]. Physiol Mol Biol Plants 19:399–407CrossRefGoogle Scholar
  13. 13.
    Jugran A, Rawat S, Dauthal P, Mondal S, Bhatt ID, Rawal RS (2013) Association of ISSR markers with some biochemical traits of Valeriana jatamansi Jones. Ind Crops Prod 44:671–676CrossRefGoogle Scholar
  14. 14.
    Rajalakshmi R, Rajalakshmi S, Parida Ajay (2017) Evaluation of the genetic diversity and Population structure in drumstick (Moringa oleifera L.) using SSR markers. Curr Sci 112:1250–1256CrossRefGoogle Scholar
  15. 15.
    Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:11–15Google Scholar
  16. 16.
    Jones JB, Wallace A (1992) Sample preparation and determination of iron in plant tissue samples. J Plant Nutr 15:2085–2108CrossRefGoogle Scholar
  17. 17.
    Yeh FC, Boyle TJB (1997) Population genetic analysis of co-dominant and dominant markers and quantitative traits. Belg J Bot 129:157Google Scholar
  18. 18.
    Liu K, Muse SV (2005) PowerMarker: an integrated analysis environment for genetic marker analysis. Bioinformatics 21:2128–2129CrossRefGoogle Scholar
  19. 19.
    Peakall R, Smouse PE (2012) GenAlEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research—an update. Bioinformatics 28:2537–2539CrossRefGoogle Scholar
  20. 20.
    Pritchard JK, Stephens M, Donnelly P (2000) Inference of population structure using multilocus genotype data. Genetics 155:945–959PubMedPubMedCentralGoogle Scholar
  21. 21.
    Evanno G, Regnaut S, Goudet J (2005) Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study. Mol Ecol 14:2611–2620CrossRefGoogle Scholar
  22. 22.
    Earl DA (2012) STRUCTURE HARVESTER: a website and program for visualizing STRUCTURE output and implementing the Evanno method. Conserv Genet Resour 4:359–361CrossRefGoogle Scholar
  23. 23.
    Perrier X, Jacquemond-Collet JP (2006) DARwin software.
  24. 24.
    Smith MR, Golden CD, Myers SS (2017) Potential rise in iron deficiency due to future anthropogenic carbon dioxide emissions. GeoHealth 1:248–257CrossRefGoogle Scholar
  25. 25.
    Prasun KD, Bhavani RV, Swaminathan MS (2014) A farming system model to leverage agriculture for nutritional outcomes. Agric Res 3:193–203CrossRefGoogle Scholar
  26. 26.
    Saini R, Saad K, Ravishankar G, Giridhar P, Shetty N (2013) Genetic diversity of commercially grown Moringa oleifera Lam. cultivars from India by RAPD, ISSR and cytochrome P450-based markers. Plant Syst Evol 299:1205–1213CrossRefGoogle Scholar
  27. 27.
    Wangsomnuk PP, Khampa S, Jogloy S, Srivong T, Patanothai A, Fu YB (2011) Assessing genetic structure and relatedness of Jerusalem artichoke (Helianthus tuberosus L.) germplasm with RAPD, ISSR and SRAP markers. Am J Plant Sci 2:753–764CrossRefGoogle Scholar
  28. 28.
    Chen SY, Dai TX, Chang YT, Wang SS, Ou SL, Chuang WL, Cheng CY, Lin YH, Lin LY, Ku HM (2013) Genetic diversity among Ocimum species based on ISSR, RAPD and SRAP markers. Aust J Crop Sci 7:1456–1462Google Scholar
  29. 29.
    Rufai S, Hanafi M, Rafii M, Ahmad S, Arolu I, Ferdous J (2013) Genetic dissection of new genotypes of drumstick tree (Moringa oleifera Lam.) using random amplified polymorphic DNA marker. Biomed Res Int 2013:1–6CrossRefGoogle Scholar
  30. 30.
    Chen L, Chen F, He S, Ma L (2014) High genetic diversity and small genetic variation among populations of Magnolia wufengensis (Magnoliaceae), revealed by ISSR and SRAP markers. Electron J Biotechnol 17:268–274CrossRefGoogle Scholar
  31. 31.
    Yu J, Jing ZB, Cheng JM (2014) Genetic diversity and population structure of Stipa bungeana, an endemic species in Loess Plateau of China, revealed using combined ISSR and SRAP markers. Genet Mol Res 13:1097–1108CrossRefGoogle Scholar
  32. 32.
    Wright S (1931) Evolution in Mendelian populations. Genetics 16:97–159PubMedPubMedCentralGoogle Scholar
  33. 33.
    Jyothi P, Atluri J, Reddi CS (1990) Pollination ecology of Moringa oleifera (Moringaceae). Proc Plant Sci 100:33–42Google Scholar
  34. 34.
    Mgendi MG, Manoko MK, Nyomora AM (2010) Genetic diversity between cultivated and non-cultivated Moringa oleifera Lam. provenances assessed by RAPD markers. J Cell Mol Biol 8:95–102Google Scholar
  35. 35.
    Shahzad U, Khan MA, Jaskani MJ, Khan IA, Korban SS (2013) Genetic diversity and population structure of Moringa oleifera. Conserv Genet 14:1161–1172CrossRefGoogle Scholar

Copyright information

© The National Academy of Sciences, India 2018

Authors and Affiliations

  1. 1.M.S. Swaminathan Research FoundationChennaiIndia
  2. 2.Institute of Life SciencesBhubaneswarIndia

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