Evolutionary Divergence of CXCR1 (Interleukin-8 Receptor A) Gene of Indian Water Buffalo (Bubalus bubalis) in Light of Molecular Evolution
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CXCR1 (Interleukin-8 Receptor A) is one of the two high-affinity receptors (IL-8RA and IL-8RB), present on the surface of neutrophils that binds to the IL-8 ligand, which results in the activation of immune response and allows the migration of immune cells to the site of inflammation. CXCR1 is strongly associated with inflammatory responses against most of the Gram-negative bacteria. The objective of the present work was to amplify, sequence and in silico characterize CXCR1 coding region of buffalo. The amplified target gene of buffalo CXCR1 was custom-sequenced, and the annotated sequence of 1130 bp having coding region of 1083 bp was submitted to DDBJ with accession number LC384988. Pairwise sequence alignment of buffalo CXCR1 and the reference cattle CXCR1 showed 95% similarity. Sequence analysis and integrative analysis of CXCR1 in 36 divergent species revealed that 7 codon positions have undergone positive selection pressure, indicating that the gene has undergone evolutionary changes over the period of time. Evolutionary analysis of the CXCR1 (MEGA 6 and Datamonkey online server) revealed that ruminant, avian, fish CXCR1 formed different clusters within the phylogenetic tree, showing evolutionary divergence.
KeywordsCXC-Chemokine receptors Interleukin-8 Receptor A Sequence analysis Characterization
Financial assistance for conducting the research work provided by UGC and RKVY is highly acknowledged.
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Conflict of interest
All authors declare that they have no conflict of interest to publish this manuscript.
- 2.Abera M, Demie B, Aragaw K, Regassa F, Regassa A (2010) Isolation and identification of Staphylococcus aureus from bovine mastitic milk and their drug resistance patterns in Adama Town, Ethiopia. J Vet Med Anim Health 2(3):29–34Google Scholar
- 15.Dige MS, Ahlawat SPS, Bhushan B, Kumar A, Kumar P, Dhama K, Bhatt P (2015) Molecular characterization of Exon-2 of CXCR2 Gene in Vrindavani Cattle. Vet Pract 16(2):291–293Google Scholar
- 16.Hedges SB (1992) The number of replications needed for accurate estimation of bootstrap P value in Phylogenetic studies. Proc R Soc 276:4261–4270Google Scholar
- 19.Dhaliwal KK, Arora JS, Mukhopadhyay CS, Dubey PP (2015) In silico characterization of functional divergence of two cathelicidin variants in Indian sheep. Evolut Bioinform Online 11:189Google Scholar