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Evolutionary Divergence of CXCR1 (Interleukin-8 Receptor A) Gene of Indian Water Buffalo (Bubalus bubalis) in Light of Molecular Evolution

  • Shiveeli Rajput
  • Jaspreet Singh AroraEmail author
  • Chandra Sekhar Mukhopadhyay
  • Jasdeep Kaur Dhanoa
  • Ramneek Verma
  • R. S. Sethi
  • Satparkash Singh
  • B. V. Sunil Kumar
  • Sikh Tejinder Singh
Research Article
  • 35 Downloads

Abstract

CXCR1 (Interleukin-8 Receptor A) is one of the two high-affinity receptors (IL-8RA and IL-8RB), present on the surface of neutrophils that binds to the IL-8 ligand, which results in the activation of immune response and allows the migration of immune cells to the site of inflammation. CXCR1 is strongly associated with inflammatory responses against most of the Gram-negative bacteria. The objective of the present work was to amplify, sequence and in silico characterize CXCR1 coding region of buffalo. The amplified target gene of buffalo CXCR1 was custom-sequenced, and the annotated sequence of 1130 bp having coding region of 1083 bp was submitted to DDBJ with accession number LC384988. Pairwise sequence alignment of buffalo CXCR1 and the reference cattle CXCR1 showed 95% similarity. Sequence analysis and integrative analysis of CXCR1 in 36 divergent species revealed that 7 codon positions have undergone positive selection pressure, indicating that the gene has undergone evolutionary changes over the period of time. Evolutionary analysis of the CXCR1 (MEGA 6 and Datamonkey online server) revealed that ruminant, avian, fish CXCR1 formed different clusters within the phylogenetic tree, showing evolutionary divergence.

Keywords

CXC-Chemokine receptors Interleukin-8 Receptor A Sequence analysis Characterization 

Notes

Acknowledgements

Financial assistance for conducting the research work provided by UGC and RKVY is highly acknowledged.

Compliance with Ethical Standards

Conflict of interest

All authors declare that they have no conflict of interest to publish this manuscript.

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Copyright information

© The National Academy of Sciences, India 2018

Authors and Affiliations

  • Shiveeli Rajput
    • 1
  • Jaspreet Singh Arora
    • 1
    Email author
  • Chandra Sekhar Mukhopadhyay
    • 1
  • Jasdeep Kaur Dhanoa
    • 1
  • Ramneek Verma
    • 1
  • R. S. Sethi
    • 1
  • Satparkash Singh
    • 1
  • B. V. Sunil Kumar
    • 1
  • Sikh Tejinder Singh
    • 2
  1. 1.School of Animal BiotechnologyGuru Angad Dev Veterinary and Animal Sciences University (GADVASU)LudhianaIndia
  2. 2.Department of Animal Breeding and GeneticsGuru Angad Dev Veterinary and Animal Sciences UniversityLudhianaIndia

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