Application of Marine Bacteria Associated with Seaweed, Ulva lactuca, for Degradation of Algal Waste
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In the present study, three marine Ulva lactuca-associated bacteria capable of producing agarase, λ-carrageenase, amylase, cellulase and protease were isolated from rocky intertidal region of Anjuna beach, Goa, India, and designated as DM1, DM5 and DM15. Based on 16S rRNA sequence analysis and biochemical tests, bacteria were identified as Vibrio brasiliensis, Bacillus subtilis and Pseudomonas aeruginosa. Bacteria DM1, DM5 and DM 15 could able to utilize seaweed waste (Sargassum powder) in seawater-based media by releasing reducing sugars, 503.3 ± 17.5 µg/ml, 491.6 ± 20 µg/ml and 376.6 ± 16 µg/ml, respectively, which was confirmed through 3,5-dinitrosalicylic acid method. Therefore, the eco-friendly reuse of seaweed waste is possible by using marine bacteria for the production of reducing sugars in ethanol-producing industry. All three bacterial isolates were found to produce indole acetic acid (IAA) at concentration 98 ± 12 µg/ml, 113.6 ± 13 µg/ml and 121.6 ± 8.5 µg/ml, respectively. Nitrogen fixation by bacterial strains was confirmed when they showed growth on artificial seawater devoid of nitrogen and comprising of 5% carrageenan as a sole source of carbon and gelling agent. Photosynthetic seaweed, Ulva lactuca, provides organic carbon and O2 for associated bacteria and associated bacteria fix atmospheric N2 and provides iron by siderophore production and synthesize hormone IAA for algal growth during their cooperative association.
KeywordsSeaweed Associated bacteria Polysaccharide Enzymes Cooperative association
The authors thank the Science and Engineering Research Board (SERB), Department of Science and Technology, Government of India, for financial support as Young Scientist Project (File Number: YSS/2014/000258). They are also thankful to Prof. S.K. Dubey, Prof. Sandeep Garg and Dr. Shyamalina Haldar (all from), Department of Microbiology, Goa University, Goa.
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Conflict of interest
The authors declare that they have no conflict of interest to publish this manuscript.
- 2.Ficko-Blean E, Préchoux A, Thomas F, Rochat Larocque R, Zhu Y, Stam M, Génicot S, Jam M, Calteau A, Viart B, Ropartz D, Pérez-Pascual D, Correc G, Matard-Mann M, Stubbs KA, Rogniaux F, Jeudy A, Barbeyron T, Médigue C, Czjzek M, Vallenet D, McBride MJ, Duchaud E, Michel G (2017) Carrageenan catabolism is encoded by a complex regulon in marine heterotrophic bacteria. Nat Commun. https://doi.org/10.1038/s41467-017-01832-6 CrossRefPubMedPubMedCentralGoogle Scholar
- 12.Imran Md, Poduval PB, Ghadi SC (2017) Bacterial degradation of algal polysaccharides in marine ecosystem. In: Naik MM, Dubey SK (eds) Marine pollution and microbial remediation. Springer, Berlin, pp 196–202Google Scholar
- 13.Shieh WY, Simidu U, Maruyama Y (1988) Nitrogen fixation by marine agar-degrading bacteria. J Gen Microbiol 134(1821–1):825Google Scholar
- 21.Kerkar V (2004) Addition to the marine algal flora of Goa. Seaweed Res Util 26:19–21Google Scholar
- 22.Liang YL, Zhang Z, Wu M, Wu Y, Feng JX (2014) Isolation, screening, and identification of cellulolytic bacteria from natural reserves in the subtropical region of China and optimization of cellulase production by Paenibacillus terrae ME27-1. BioMed Res Int. https://doi.org/10.1155/2014/512497 CrossRefPubMedPubMedCentralGoogle Scholar
- 26.Mohite B (2013) Isolation and characterization of indole acetic acid (IAA) producing bacteria from rhizospheric soil and its effect on plant growth. J Soil Sci Plant Nutr 13:638–649Google Scholar
- 29.Bergey DH, Kreig NR, Holt JG (1984) Bergey’s manual of systematic bacteriology. Williams & Wilkins, BaltimoreGoogle Scholar