Persistence of V. cholerae O139 Biofilm Against Physical, Chemical and Antibiotic Lethal Challenges
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Since its emergence, cholera caused by the bacterium Vibrio cholerae remains as a significant threat to human health. The continued persistence of this pathogen against many unfavourable conditions made challenging to eradicate cholera, especially in the developing countries. In the hostile conditions, the bacterium is known to form a self-enclosed polymeric structure called ‘biofilm’ which serves as a major factor responsible for its persistence and transmission. Hence, the authors aimed to understand the effectiveness of relevant physical, chemical and antibiotic treatments against the biofilm of this bacterium. For the study, the biofilm of V. cholerae O139 wild type and its isogenic Tn5-mutants that differ in their biofilm phenotype (biofilm proficient and biofilm deficient) were exposed to different levels of pH, salinity, temperature, UV radiation, H2O2, chlorination, and antibiotics. It was observed that biofilm culture of both wild type and the biofilm-proficient mutant exhibited detectable survival rate up to pH 2.0, salinity 3.5 M, temperature 50 °C, H2O2 80 mM, NaOCl 12.5 mg/L and at several folds increased antibiotic concentration (Ciprofloxacin 2 mg/L, and Doxycycline 256 mg/L) as compared to the biofilm defective mutant and the planktonic cultures of respective strains. As the biofilm of the cholera pathogen or the cholera biofilm resists several lethal challenges, it is essential to target the biofilm and its residents for the complete destruction of the infection source and thereby to prevent transmission of cholera.
KeywordsV. cholerae Cholera biofilm Persistence Tn5-mutants Lethal treatments
The authors are thankful to the Director, Defence Research and Development Establishment (DRDE) Gwalior, the Government of India (DRDE/MB/011/2018), for providing all the facilities and financial support to carry out this work. They are extremely grateful to Prof. Eric V. Stabb, Department of Microbiology, University of Georgia, Athens Georgia, for the kind gift of pEVS 168, pEVS104 vectors, and E. coli DH5 α/λpir host, which were used in the construction biofilm, altered Tn5 mutants of V. cholerae O139. This study was supported by Institutional fund provided by Defence R&D Establishment, Gwalior, Ministry of Defence, the Government of India.
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Conflict of interest
The authors declare that they have no conflict of interest.
- 2.World Health Organization (2017) Diarrhoeal diseases. http://www.who.int/mediacentre/factsheets/fs330/en/. Accessed 2 May 2017
- 8.Yildiz FH, Schoolnik GK (1999) Vibrio cholerae O1 El Tor: identification of a gene cluster required for the rugose colony type, exopolysaccharide production, chlorine resistance, and biofilm formation. Proc Natl Acad Sci USA 96:4028–4033. https://doi.org/10.1073/pnas.96.7.4028 CrossRefPubMedGoogle Scholar
- 15.Clinical and Laboratory Standards Institute (2017) Performance standards for antimicrobial susceptibility testing: twenty-seven informational supplement M100–S27. CLSI, Wayne, PA, USA, 2017Google Scholar
- 16.Faruque SM, Biswas K, Udden SM, Ahmad QS, Sack DA, Nair GB, Mekalanos JJ (2006) Transmissibility of cholera: invivo-formed biofilms and their relationship to infectivity and persistence in the environment. Proc Natl Acad Sci USA 103:6350–6355. https://doi.org/10.1073/pnas.0601277103 CrossRefPubMedGoogle Scholar
- 21.Wilson BR, Roessler PF, VanDellen E, Abbaszadegan M, Gerba CP (1992) Coliphage MS-2 as a UV water disinfection efficacy test surrogate for bacterial and viral pathogens. In: Proceedings, water quality technology conference, November 15–19 Toronto, Canada, pp 219–235Google Scholar
- 28.International Commission of Microbiological Specification for Food (1974) Microorganisms in food -2. University of Toronto Press, TorontoGoogle Scholar
- 30.World Health Organization & International Programme on Chemical Safety (1996) Guidelines for drinkingwater quality, 2nd ed. Health criteria and other supporting information, vol 2. World Health Organization, Geneva. http://www.who.int/iris/handle/10665/38551