Characterization of Bacillus Species with Keratinase and Cellulase Properties Isolated from Feather Dumping Soil and Cockroach Gut
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The present study was conducted with an objective to isolate and screen bacteria with dual characteristics of cellulase and keratinase production from soil of feather dumping site and from the gut of cockroaches which inhabit these sites. By using standard microbiological protocols and techniques, 13 bacteria (K1–K13) were isolated from soil samples and 18 isolates from cockroach gut (K14–K31), and further these isolates were screened for keratinase activity. The positive isolates with keratinase activity were subsequently screened for cellulase production. The enzymatic activity of both keratinase and cellulase was quantified followed by characterization and identification of these positive isolates through 16S rRNA genetic region sequencing. The isolate showing maximum keratinase and cellulase enzyme activity was selected and tested for degradation potential using animal skin with hairs as a natural keratin. The present study demonstrates the waste management ability of Bacillus sp., with dual characteristics of cellulase and keratinase production isolated from cockroach gut.
KeywordsCellulase activity Hydrolysis capacities Waste management 16S rDNA Enzyme
The authors thankfully acknowledge the support of Defence Research & Development Organization, Ministry of Defence, for intramural research funds. They also extend their sincere thanks to Dr. Sibnarayan Datta, Scientist, DRL and Dr. Yangchen Bhutia, Scientist, DRL, Dr. Narendra Kumar, DAVV & Director Defence Research Laboratory, Tezpur, for their kind support and help in manuscript preparation.
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Conflict of interest
The authors declare no conflict of interest to publish this manuscript.
- 3.Jaouadi NZ, Rekik H, Badis A, Trabelsi S, Belhoul M et al (2013) Biochemical and molecular characterization of a serine keratinase from Brevibacillus brevis us575 with promising keratin-biodegradation and hide-dehairing activities. PLoS ONE 8:e76722. https://doi.org/10.1371/journal.pone.0076722 CrossRefPubMedPubMedCentralGoogle Scholar
- 6.Han M, Luo W, Gu Q, Yu X (2012) Isolation and characterization of a keratinolytic protease from a feather-degrading bacterium Pseudomonas aeruginosa C11. Afr J Microbiol Res 6:2211–2222Google Scholar
- 10.Sharma S, Chatterjee S, Datta S, Prasad RK, Sharma A, Dubey D, Vairale MG, Veer V (2015) Isolation and characterization of cellulose degrading bacteria of termite gut from north eastern region of India. South Asian J Exp Biol 5:283–290Google Scholar
- 13.El-Ayouty YM, EL-said A, Salama AM (2012) Purification and characterization of a keratinase from the feather-degrading cultures of Aspergillus flavipes. Afr J Biotechnol 11:2313–2319Google Scholar
- 17.Kani PT, Subha K, Madhanraj P, Senthilkumar G, Panneerselvam A (2012) Degradation of chicken feathers by Leuconostoc sp. and Pseudomonas microphilus. Euro J Exp Biol 2:358–362Google Scholar
- 27.Chatterjee S, Sharma S, Prasad RK, Datta S, Dubey D, Meghvansi MK, Vairale MG, Veer V (2015) Cellulase enzyme based biodegradation of cellulosic materials: an overview. South Asian J Exp Biol 5:271–282Google Scholar