In Vitro and In Vivo Study of Argyreia speciosa on Chronic Gastric Ulceration and Metabolic Studies
- 15 Downloads
Chronic gastric ulcer being the most prevalent gastrointestinal disorder is considered a major cause of gastric cancer. Large numbers of synthetic drugs ranging from proton-pump inhibitors and H2 receptor antagonist to cytoprotective agents are available, but they have major side effects. Inadequacy of these synthetic drugs forces to develop new herbal-based drugs active against chronic gastric ulcer. In the present study, antiulcer activity of butanolic extract of Argyreia speciosa Linn. f., Sweet: Family Convolvulaceae (b-ASL) was investigated against acetic-acid-induced chronic gastric ulcer rat models. The effect of different dose of butanolic extract of A. speciosa was studied for antioxidant, anti-secretory, and cytoprotective activities. Extract administration significantly increased the gastric PGE2 content. The further experiments including the in vitro metabolic stability and in vivo pharmacokinetics gave a clear indication that butanolic extract of A. speciosa (200 mg/kg) is an orally active and safe contender of antiulcer agents. It can be an important target for further studies under chronic ulcer management system.
KeywordsArgyreia speciosa Chronic gastric ulcer Flavonol Metabolic stability
The authors are thankful to the Director, CSIR-National Botanical Research Institute, Lucknow, India for providing necessary facilities. They are grateful to Department of Science and Technology (DST), Ministry of Science and Technology, New Delhi, India, for providing DST-INSPIRE fellowship.
Compliance with Ethical Standards
Conflict of interest
The authors declare that they have no competing interests.
- 2.Bindu S, Pal C, Dey S, Goyal M, Alam A, Iqbal MS, Dutta S, Sarkar S, Kumar R, Maity P, Bandyopadhyay U (2011) Translocation of heme Oxygenase-1 to Mitochondria is a novel cytoprotective mechanism against non-steroidal anti-inflammatory drug-induced mitochondrial oxidative stress, apoptosis, and gastric mucosal injury. J Biol Chem 286(45):39387–39402. https://doi.org/10.1074/jbc.M111.279893 CrossRefPubMedPubMedCentralGoogle Scholar
- 3.Pal C, Bindu S, Dey S, Alam A, Goyal M, Iqbal MS, Maity P, Adhikari SS, Bandyopadhyay U (2010) Gallic acid prevents nonsteroidal anti-inflammatory drug induced gastropathy in rat by blocking oxidative stress and apoptosis. Free Radic Biol Med 49(2):258–267. https://doi.org/10.1016/j.freeradbiomed.2010.04.013 CrossRefPubMedGoogle Scholar
- 18.Mishra V, Agrawal M, Onasanwo SA, Madhur G, Rastogi P, Pandey HP, Palit G, Narender T (2013) Anti-secretory and cyto-protective effects of chebulinic acid isolated from the fruits of Terminalia chebula on gastric ulcers. Phytomedicine 20(6):506–511. https://doi.org/10.1016/j.phymed.2013.01.002 CrossRefPubMedGoogle Scholar
- 20.Bogaards JJ, Bertrand M, Jackson P, Oudshoor MJ, Weaver RJ, van Bladeren PJ, Walther B (2000) Determining the best animal model for human cytochrome P450 activities: a comparison of mouse, rat, rabbit, dog, micropig, monkey and man. Xenobiotica 30:1131–1152. https://doi.org/10.1080/00498250010021684 CrossRefPubMedGoogle Scholar
- 21.Finney DJA (1952) Statistical treatment of the sigmoidal response curve, vol 78(3). Cambridge Univ Press, New York, pp 388–390. http://www.jstor.org/stable/41139025
- 23.Nartey ET, Ofosuhene M, Kudzi W, Agbale CM (2012) Antioxidant and gastric cytoprotective prostaglandins properties of Cassia sieberiana roots bark extract as an anti-ulcerogenic agent. BMC Complement Altern Med 12:65–74. https://doi.org/10.1186/1472-6882-12-65 CrossRefPubMedPubMedCentralGoogle Scholar