Releasing Digestive Enzymes by Brain Peptides in the Larvae of Pieris brassicae L. (Lepidoptera: Pieridae)
- 50 Downloads
Neuropeptides synthesized by insect neurosecretory cells are engaged in several physiological process like immunity, feeding and reproduction so they deserve to be undertaken in subsequent experiments for discovery and manipulation regarding applied objectives. Due to importance of insect ability to utilize plant tissues, the present study was conducted to extract brain peptides in the fifth instar larvae of Pieris brassicae via High performance liquid chromatography and to identify their role in induction of digestive enzyme syntheses. Five peaks were determined and named as fractions 1, 2, 3, 4 and 5. The midguts of starved fifth larval instars were treated by each fraction of brain extract in vitro. The highest activities of α-amylase and lipase were found in the midgut treated by fractions 4 and 1, respectively while the fraction number 5 imposed the highest activities of glucosidases. Fraction 2 led to the highest activities of trypsin, chymotrypsin and elastase as the given serine proteinases as well as the two exopeptidases namely amino- and carboxypeptidases. Moreover, midgut treatments by different concentrations of TLCK (N-p-Tosyl-l-lysine methyl ester hydrochloride), TPCK (Tosyl phenylalanyl chloromethyl ketone), AEBSF (4-(2-Aminoethyl) benzenesulfonyl fluoride hydrochloride) and Acarbose significantly decreased the activities of α-amylase, trypsin, chymotrypsin and elastase by the calculated IC50 values of 0.287, 0.439, 0.288 and 0.361 mM, respectively. The findings demonstrated that different neuropeptides would be engaged in enzymatic release of P. brassicae hence, manipulation of peptide receptors can change physiological process of insects and opens a selective strategy to pest control.
KeywordsPieris brassicae Brain peptide Digestive enzyme High performance liquid chromatography Manipulation of peptide receptors
The authors would like to appreciate assistances of Ms. Minoo Sajjadian for HPLC analysis. The present study was supported by University of Guilan, Iran.
Compliance with Ethical Standards
Conflict of interest
The authors declare that there is no conflict of interest among the authors regarding publication of this manuscript.
Ethical Standard Statement
The experiments was conducted based on the rules on compliance with ethical statements.
- 2.Chapman RF (2013) The insects: structure and function, 5th edn. Cambridge University Press, CambridgeGoogle Scholar
- 8.Karuppaiah V, Sujithra M (2013) Insect neuropeptides: as potential targets for pest control, research news for U (RNFU) ISSN: 2250–3668, vol 10Google Scholar
- 12.Frugoni JAC (1957) Tampone universale di Britton e Robinson a forza ionica costante. Gazz Chim Ital 87:403–407Google Scholar
- 16.Harshini S, Sreekumar S (2001) Isolation and partial purification of the digestive enzyme-release peptide hormone from the midgut of larvae of Opisina arenosella Walk. (Lepidoptera: Cryptophasidae. In: Muraleedharan K et al (eds) Advances in entomology. Association for Advancement of Entomology, Trivandrum, pp 191–197Google Scholar