Impact Assessment of Cetyltrimethylammonium Bromide Treated Organically Modified Nanoclay-Polymer Composite on Soil Enzyme Activity and Root Volume
- 55 Downloads
The effect of organically modified nanoclay-polymer composite was evaluated on soil enzyme activity and root volume at different growth stages viz. crown root initiation, tillering and panicle initiation of wheat (variety: HD 3059). Nanoclay-polymer composite was synthesized by in -situ polymerization of acrylic acid-acrylamide in presence of cetyltrimethylammonium bromide @ 60% neutralization degree and 0.5% cross-linker level and subsequently loaded with di-ammonium phosphate fertilizer (14 g di-ammonium phosphate per unit weight of nanoclay-polymer composite). Phosphorus loaded nanoclay-polymer composite and conventional diammonium phosphate fertilizer were applied (P dose-40 mg P2O5 kg−1 soil) to two types of soil (Alfisol and Inceptisol). Soil enzyme activity and root volume were studied independently with a factorial completely randomized design experiment. Results revealed that dehydrogenase enzyme activity in modified nanoclay-polymer composite increased by + 26.3 and + 19% over conventional fertilizer in Alfisol and Inceptisol respectively. Acid phosphatase activity (9.25 µg p-nitrophenol g−1 soil h−1) was highest at panicle initiation stage in Inceptisol whereas alkaline phosphatase activity was more at tillering in both soils. Root volume also increased by + 16.8% on applying nanoclay-polymer composite as compared to conventional fertilizer in Alfisol. The corresponding data in Inceptisol was + 13.1%. Therefore, it was concluded that nanoclay-polymer composite prepared from organoclay has a positive effect on soil enzyme activity as well as root volume.
KeywordsOrganoclay Phosphorus dehydrogenase Phosphatase Soil health
The first author is grateful to Indian Council of Agricultural Research (ICAR), New Delhi for providing financial support as Junior Research Fellowship (JRF) during her research programme and Indian Agricultural Research Institute (IARI), New Delhi for providing all facilities for successful completion of the research work.
Compliance with Ethical Standards
Conflict of interest
The authors have no conflict of interest for publishing this manuscript.
- 4.Basak BB, Pal S, Datta SC (2012) Use of modified clays for retention and supply of water and nutrients. Curr Sci 102:1272–1278Google Scholar
- 9.Kujur M, Gartia SK, Patel AK (2012) Quantifying the contribution of different soil properties on enzyme activities in dry tropical ecosystems. ARPN J Agric Biol Sci 7(9):763–772Google Scholar
- 10.Harvey PJ, Xiang M, Palmer JM (2002) Extracellular enzymes in the rhizosphere. In: Proceedings of inter-cost workshop on soil-microbe-root interactions: maximizing phytoremediation/bioremediation. Grainau, Germany, 23–25Google Scholar
- 11.Burns RG (1978) Enzyme activity in soil: some theoretical and practical considerations. In: Burns RG (ed) Soil enzymes. Academic Press, London, pp 295–340Google Scholar
- 12.Glinski J, Stepniewski W (1985) Soil aeration and its role for plants. CRC, Boca RatonGoogle Scholar
- 14.Jackson ML (1956) Soil chemical analysis-advanced course, Published by the author. Department of Soil Science, University of Wisconsin, Madison, USAGoogle Scholar
- 16.Jackson ML (1973) Soil chemical analysis. Prentice Hall of India Private Limited, New DelhiGoogle Scholar
- 19.Subbiah BV, Asija GL (1956) A rapid procedure for the determination of available nitrogen in soils. Curr Sci 25:259–260Google Scholar
- 20.Olsen SR, Cole CV, Watanable FS, Dean LA (1954) Estimate of available phosphorus in soils by extraction with sodium bicarbonate. USDA Circ 9398:1–9Google Scholar
- 21.Hanway JJ, Heidel H (1952) Soil analysis methods as used in Iowa State College (Agricultural Bulletin No. 57). Iowa State College, Iowa, pp 1–13Google Scholar
- 25.Snedecor GW, Cochran WG (1980) Statistical methods, 7th edn. The Lowa State University, Press America, LowaGoogle Scholar
- 27.Bhattacharya SS, Aadhar M (2014) Studies on preparation and analysis of organoclay nano particles. Res J Eng Sci 3(3):10–16Google Scholar
- 28.Mandal N (2014) Development of biodegradable clay-nanocomposites for controlled deliver of Zn in wheat and rice rhizosphere. Ph.D. thesis, Division of Soil Science and Agricultural Chemistry, Indian Agricultural Research Institute, New Delhi, IndiaGoogle Scholar