Chromate Reduction by Purple Non Sulphur Phototrophic Bacterium Rhodobacter sp. GSKRLMBKU–03 Isolated from Pond Water

  • K. RajyalaxmiEmail author
  • Ramchander Merugu
  • S. Girisham
  • S. M. Reddy
Research Article


A purple non sulphur phototrophic bacterium (PNSB) Rhodobacter sp. GSKRLMBKU–03 was isolated from pond water of Thadoba forest, Chandrapoor District, India and its ability to reduce hexavalent chromium was discussed in the present study. Both free and immobilized cells of this bacterium were employed for chromate reduction. Immobilization of cells resulted in enhanced reduction of chromate. Sodium alginate entrapment could reduce chromate up to 40 μM, while free cells of Rhodobacter sp. GSKRLMBKU–03 could reduce about 35 µM of chromate on 8th day of its incubation under anaerobic light conditions. Both free and immobilized cells could reduce chromate even on the 20th day. Optimal growth and chromate reduction was observed on 8th day of its incubation period. The final pH of the free cells and immobilized cells in growth medium was recorded in the range of 7.5 ± 0.25–7.6 ± 0.15 at optimal incubation period respectively. The optimal growth of free cells and immobilized cells in terms of dry cell weight (DCW) was recorded as 1.80 ± 0.15 and 2.2 ± 0.20 g/L respectively. The optimal growth observed upon complete chromate reduction. The results are expressed in mean and standard deviations which are statistically significant at P ≤ 0.05 level. The employment of Rhodobacter sp. in bioremediation to detoxify chromium in large-scale systems is proposed.


Chromate reduction Heavy metal Rhodobacter sp. GSKRLMBKU–03 Immobilization 



This research has been financially supported by UGC under SAP, New Delhi, India and all the facilities provided by the Head, Department of Microbiology, Kakatiya University for which the authors are grateful.

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.


  1. 1.
    Williams JW, Silver S (1984) Bacterial resistance and detoxification of heavy metals. Enzyme Microb Technol 6:530–537CrossRefGoogle Scholar
  2. 2.
    Shanker AK, Cervantes C, Loza-Tavera H, Avuainayagam S (2005) Chromium toxicity in plants. Review article. Environ Int 31:739–753CrossRefGoogle Scholar
  3. 3.
    Cieslak-Golonka M (1995) Toxic and mutagenic effects of chromium(VI). A review. Polyhedron 15:3667–3689CrossRefGoogle Scholar
  4. 4.
    Bagchi D, Stohs SJ, Downs BW, Bagchi M, Preuss HG (2002) Cytotoxicity and oxidative mechanisms of different forms of chromium. Toxicology 180:5–22CrossRefGoogle Scholar
  5. 5.
    Gadd GM (2000) Bioremedial potential of microbial mechanisms of metal mobilization and immobilization. Curr Opin Biotechnol 11:271–279CrossRefGoogle Scholar
  6. 6.
    Daulton TL, Little BJ, Lowe K, Meehan JJ (2002) Electron energy loss spectroscopy techniques for the study of microbial chromium(VI) reduction. J Microbiol Methods 50:39–54CrossRefGoogle Scholar
  7. 7.
    Smiejan A, Wilkinson KJ, Rossier C (2003) Cadmium bioaccumulation by a freshwater bacterium, Rhodospirillum rubrum. Environ Sci Tech 37:701–706CrossRefGoogle Scholar
  8. 8.
    Watanabe M, Kawahara K, Sasaki K, Noparatnaraporn N (2003) Biosorption of cadmium ion using a photosynthetic bacterium, Rhodobacter sphaeroides S and a marine photosynthetic bacterium, Rhodovulum sp. and their biosorption kinetics. J Biosci Bioeng 95:374–378CrossRefGoogle Scholar
  9. 9.
    Buccolieri A, Italino F, Dell’Atti A, Buccolieri G, Giotta L, Agostiano A, Milano F, Trotta M (2006) Testing the photosynthetic bacterium Rhodobacter sphaeroides as heavy metal removal tool. Ann Chim 96:195–203CrossRefGoogle Scholar
  10. 10.
    Gad El–Rab SMF, Shoreit AAF, Fukumori Y (2006) Effects of cadmium stress on growth, morphology, and protein expression in Rhodobacter capsulatus B10. Biosci Biotechnol Biochem 70:2394–2402CrossRefGoogle Scholar
  11. 11.
    Italiano F, Buccolieri A, Giotta L, Agostiano A, Valli L, Milano F, Trotta M (2009) Response of the carotenoidless mutant Rhodobacter sphaeroides growing cells to cobalt and nickel exposure. Int Biodeter Biodegrad 63(7):948–957CrossRefGoogle Scholar
  12. 12.
    Borsetti F, Toninello A, Zannoni D (2003) Tellurite uptake by cells of the facultative phototroph Rhodobacter capsulatus is a delta pH-dependent process. FEBS Lett 554:315–318CrossRefGoogle Scholar
  13. 13.
    Rajyalaxmi K, Ramchander M, Girisham S, Reddy SM (2015) Phosphate solubilization by Allochromatium sp. GSKRLMBKU–01 isolated from marine water of Visakhapatnam. Int J Appl Bio Pharm Technol 6:1–6Google Scholar
  14. 14.
    Zadvorny OA, Zorin NA, Gogotov IN (2006) Transformation of metals and metal ions by hydrogenases from photrophic bacteria. Arch Microbiol 184:279–285CrossRefGoogle Scholar
  15. 15.
    Philip L, Iyengar L, Venkobachar C (1998) Cr(VI) reduction by Bacillus coagulans isolated from contaminated soils. J Environ Eng 124:1165–1170CrossRefGoogle Scholar
  16. 16.
    Merugu Ramchander, Pratap Rudra MP, Thirupathaiah A, Girisham S, Reddy SM (2011) Chromate reduction by a purple non sulphur phototrophic bacterium Rhodobacter capsulatus KU002 isolated from tannery Effluents. J Pure Appl Microbiol 5(2):66–69Google Scholar
  17. 17.
    Ramchander M, Rajyalaxmi K, Girisham S, Reddy SM (2013) Chromate reduction by purple non sulphur phototrophic bacterium Rhodopseudomonas palustris KU003 isolated from tannery effluents. Int J Environ Bioenergy 6(3):187–192Google Scholar
  18. 18.
    Sasikala C, Ramana CV (1995) Biotechnological potentials of anoxygenic phototrophic bacteria. I. Production of single cell protein, vitamins, ubiquinones, hormones and enzymes and use in waste treatment. Adv Appl Microbiol 41:173–226CrossRefGoogle Scholar
  19. 19.
    Ramchander M, Pratap Rudra MP, Girisham S, Reddy SM (2012) Biotechnological applications of purple non sulphur phototrophic bacteria: a minireview. Int J Appl Biol Pharm Technol 3:376–384Google Scholar
  20. 20.
    Ramchander M, Girisham S, Reddy SM (2010) Production of PHB (polyhydroxy butyrate) by Rhodopseudomonas palustris KU003 under nitrogen limitation. Int J Appl Bio Pharm Technol 2:686–688Google Scholar
  21. 21.
    Ramchander M, Girisham S, Reddy SM (2010) Bioproduction of hydrogen by Rhodobacter capsulatus KU002 isolated from leather industry effluents. Inter J Hydr Energy 35:9591–9697CrossRefGoogle Scholar
  22. 22.
    Nepple B, Kessi J, Bachofen R (2002) Chromate reduction by Rhodobacter sphaeroides. J Ind Microb Biotechnol 25:198–203CrossRefGoogle Scholar
  23. 23.
    Biebl H, Pfennig N (1981) Isolation of members of the family Rhodospirillaceae. In: Starr MP, Stolp H, Truper HG, Balows A, Schlegel HG (eds) The prokaryotes. Springer, New York, pp 167–273Google Scholar
  24. 24.
    Holt JG, Krieg NR, Sneath PHA, Staley JT, Williams ST (1994) Bergey’s manual of determinative microbiology. Williams and Wilkins, MarylandGoogle Scholar
  25. 25.
    Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance and maximum parsimony methods. Mol Biol Evol 28:2731–2739CrossRefGoogle Scholar
  26. 26.
    Johnsen A, Flink JM (1986) Influence of alginate properties and gel reinforcement on fermentation characteristics of immobilized yeast cells. Enzyme Microb Technol 8:737–748CrossRefGoogle Scholar
  27. 27.
    Adinaryana K, Jyothi B, Elliah P (2005) Production of alkaline protease with immobilized cells of Bacillus subtilis PE 11 in various matrices by entrapment technique. AAPS Pharm Sci Tech 6:391–397CrossRefGoogle Scholar
  28. 28.
    Greenberg A, Clescerl L, Eaton A (1992) Standard methods for the examination of water and wastewater, 18th edn. American Public Health Association, Washington DCGoogle Scholar
  29. 29.
    Pfennig N, Trüper HG (1981) Isolation of members of the families Chromatiaceae and Chlorobiaceae. In: Starr MP, Stolp H, Trüper HG, Balows A, Schlegel HG (eds) The prokaryotes: a handbook on habitats, isolation, and identification of bacteria. Springer, Berlin, pp 279–289CrossRefGoogle Scholar
  30. 30.
    Italiano F, Rinalducci S, Agostiano A, Zolla L, De Leo F, Ceci LR, Trotta M (2012) Changes in morphology, cell wall composition and soluble proteome in Rhodobacter sphaeroides cells exposed to chromate. Biometals 25:939–949CrossRefGoogle Scholar

Copyright information

© The National Academy of Sciences, India 2017

Authors and Affiliations

  1. 1.Department of MicrobiologyKakatiya UniversityWarangalIndia
  2. 2.Department of BiochemistryMahatma Gandhi UniversityNalgondaIndia
  3. 3.Department of Botany and MicrobiologyKakatiya UniversityWarangalIndia

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