Growth and Proximate Composition of Scenedesmus obliquus and Selenastrum bibraianum Cultured in Different Media and Condition
- 60 Downloads
Microalgae are natural producers of various biochemical compounds such as protein, lipid, carotenoids and fatty acids. However, the biochemical composition of microalgae can vary depending on species, culture conditions as well as composition of culture media. This research aims to compare the growth and proximate composition of Scenedesmus obliquus and Selenastrum bibraianum cultured in different media and culture conditions. In the present study, S. obliquus and S. bibraianum were cultured in two different media (BBM and Bristol) under control and natural conditions. Results revealed the highest growth of S. obliquus when cultured in BBM under control condition. Nonetheless, S. obliquus accredited the highest lipid (42.6 ± 0.01%) and carbohydrate content (27.7 ± 0.02%) when cultured in Bristol media under control condition. On the other hand, the highest protein content (44.7 ± 0.00%) was obtained in S. bibraianum when cultured in Bristol media under natural condition. The findings from this study can be used in cultivation of S. obliquus and S. bibraianum using the optimum growth condition for specific purposes of either to obtain a higher biomass growth or better biochemical conformation.
KeywordsMicroalgae Proximate composition Growth Media Culture condition
The present study was supported by the Ministry of Higher Education, Malaysia, through the Fundamental Research Grant Scheme (FRGS) Project No. FRGS/1/2015/WAB01/UMT/03/6.
Compliance with Ethical Standards
Conflict of interest
All the authors declare that they have no conflict of interests.
- 1.Hoek C, Mann D, Jahns HM (1995) Algae: an introduction to phycology. Cambridge University Press, CambridgeGoogle Scholar
- 2.Alabi AO, Tampier M, Bibeau E (2009) Microalgae technologies and processes for biofuels/bioenergy production in British Columbia, current technology, suitability and barriers to implementation. Final Report, the British Columbia Innovation Council. Seed Science PressGoogle Scholar
- 5.Khatoon H, Rahman NA, Banerjee S, Harun N, Suleiman SS, Zakaria NH, Lananan F, Abdul Hamid SH, Endut A (2014) Effects of different salinities and pH on the growth and proximate composition of Nannochloropsis sp. and Tetraselmis sp. isolated from South China Sea cultured under control and natural condition. Int Biodeterior Biodegrad 95:11–18CrossRefGoogle Scholar
- 6.Li Y (2011) Inexpensive culturing of freshwater algae in a simulated warm environment using chicken manure medium. Chalmers University of Technology, Goteborg, pp 1–46Google Scholar
- 7.Toyub MA, Miah MI, Habib MAB, Rahman MM (2008) Growth performance and nutritional value of Scenedesmus obliquus cultured in different concentrations of sweetmeat factory waste media., Bangladesh. J Anim Sci 1(37):86–93Google Scholar
- 8.Sigea EG (2010) A key to the more frequently occurring freshwater algae. In: Freshwater algae: identification and use as bioindicators. Wiley, London, pp 137–254Google Scholar
- 9.Stein J (1980) Handbook of phycological methods: Culture methods and growth measurements. Cambridge University Press, Cambridge, p 448Google Scholar
- 10.Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:265–275Google Scholar
- 11.Marsh JB, Weinstein DB (1966) Simple charring method for determination of lipids: notes on methodology. J Lipid Res 7:574–576Google Scholar
- 17.Çelekli A, Balci M (2009) The influence of different phosphate and nitrate concentrations on growth, protein and chlorophyll a content of Scenedesmus obliquus. Fresenius Environ Bull 17:1363–1366Google Scholar
- 20.Andersen AR (2005) Recipe for freshwater and seawater media; algal culturing technique. Academic Press, New York, pp 437–438Google Scholar
- 21.Work VH, Radakovits R, Jinkerson RE, Meuser JE, Elliott LG, Vinyard DJ, Laurens LML, Dismukesn GC, Posewitz MC (2010) Increased lipid accumulation in the Chlamydomonas reinhardtii sta7-10 starchless isoamylase mutant and increased carbohydrate strains. PubMed Central 9(18):1251–1261Google Scholar
- 23.Shaleh M, Raehanah S (2004) Optimum parameters for both indoor and outdoor propagation of microalgae, Chlorella vulgaris and Isochrysis galbana sp. Universiti Putra MalaysiaGoogle Scholar