Growth and Proximate Composition of Scenedesmus obliquus and Selenastrum bibraianum Cultured in Different Media and Condition

  • Helena KhatoonEmail author
  • Norazira Abdu Rahman
  • Siti Suhada Suleiman
  • Sanjoy Banerjee
  • Ambok Bolong Abol-Munafi
Research Article


Microalgae are natural producers of various biochemical compounds such as protein, lipid, carotenoids and fatty acids. However, the biochemical composition of microalgae can vary depending on species, culture conditions as well as composition of culture media. This research aims to compare the growth and proximate composition of Scenedesmus obliquus and Selenastrum bibraianum cultured in different media and culture conditions. In the present study, S. obliquus and S. bibraianum were cultured in two different media (BBM and Bristol) under control and natural conditions. Results revealed the highest growth of S. obliquus when cultured in BBM under control condition. Nonetheless, S. obliquus accredited the highest lipid (42.6 ± 0.01%) and carbohydrate content (27.7 ± 0.02%) when cultured in Bristol media under control condition. On the other hand, the highest protein content (44.7 ± 0.00%) was obtained in S. bibraianum when cultured in Bristol media under natural condition. The findings from this study can be used in cultivation of S. obliquus and S. bibraianum using the optimum growth condition for specific purposes of either to obtain a higher biomass growth or better biochemical conformation.


Microalgae Proximate composition Growth Media Culture condition 



The present study was supported by the Ministry of Higher Education, Malaysia, through the Fundamental Research Grant Scheme (FRGS) Project No. FRGS/1/2015/WAB01/UMT/03/6.

Compliance with Ethical Standards

Conflict of interest

All the authors declare that they have no conflict of interests.


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Copyright information

© The National Academy of Sciences, India 2017

Authors and Affiliations

  • Helena Khatoon
    • 1
    Email author
  • Norazira Abdu Rahman
    • 2
  • Siti Suhada Suleiman
    • 2
  • Sanjoy Banerjee
    • 3
  • Ambok Bolong Abol-Munafi
    • 1
  1. 1.Institute of Tropical AquacultureUniversiti Malaysia TerengganuKuala TerengganuMalaysia
  2. 2.School of Fisheries and Aquaculture SciencesUniversiti Malaysia TerengganuKuala TerengganuMalaysia
  3. 3.Institute of BioscienceUniversiti Putra Malaysia (UPM)Serdang, SelangorMalaysia

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