Evaluation of Acetamiprid Mediated Oxidative Stress and Pathological Changes in Male Rats: Ameliorative Effect of Curcumin

  • Sagar Doltade
  • Milindmitra LonareEmail author
  • Sachin Raut
  • Avinash Telang
Research Article


Present study was undertaken to evaluate the acetamiprid-mediated toxicity and possible protective effect of curcumin in rats. Rats were divided into seven different groups. They received curcumin (100 mg/kg, b.wt. p.o.) and acetamiprid (26.25 and 105 mg/kg b.wt., p.o.) alone and in their combinations for period of 28 days. Lipid peroxidation and antioxidant enzyme activities were determined in liver, kidney and brain and cellular changes histopathologically. Study shows that administration of acetamiprid caused a significant induction in oxidative damage in liver, kidney and brain as evidenced by increased level of lipid peroxidation (LPO) and altered activities of reduced glutathione (GSH), catalase (CAT), superoxide dismutase (SOD) and glutathione reductase (GR). Co-administration of curcumin with acetamiprid significantly decreased LPO, caused marked restoration in the non-enzymatic (GSH) and enzymatic antioxidants system (CAT, SOD and, GR) and protected from acetamiprid-mediated structural alterations of liver, kidney and brain. The results of present study indicates that curcumin ameliorate the acetamiprid-induced toxic damage to vital organ mediated via oxidative stress.


Acetamiprid Oxidative stress Curcumin Antioxidants 



The authors are thankful to the Director, Indian Veterinary Research Institute, Izzatnagar, Bareilly and Indian Council of Agriculture and Research, New Delhi, India for providing Junior Research Fellowship for research. They also acknowledge M/S Gharda Chemicals, Mumbai, India for generously providing technical grade acetamiprid.

Compliance with Ethical Standards

Conflict of interest

The authors declare that there is no conflict of interest.


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Copyright information

© The National Academy of Sciences, India 2017

Authors and Affiliations

  • Sagar Doltade
    • 1
  • Milindmitra Lonare
    • 1
    • 4
    Email author
  • Sachin Raut
    • 2
  • Avinash Telang
    • 3
  1. 1.Division of Veterinary Pharmacology and ToxicologyIndian Veterinary Research InstituteIzatnagar, BareillyIndia
  2. 2.Division of Veterinary PathologyIndian Veterinary Research InstituteIzatnagar, BareillyIndia
  3. 3.Centre for Animal Disease Research and DiagnosisIndian Veterinary Research InstituteIzatnagar, BareillyIndia
  4. 4.College of Veterinary SciencesGADVASULudhianaIndia

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