Advertisement

Antioxidant and Antimicrobial Potential of Natural Colouring Pigment Derived from Bixa orellana L. Seed Aril

  • Vinod Kumar NathanEmail author
  • Mary Esther Rani
  • Gunaseeli Rathinasamy
  • Kannan Narayanan Dhiraviam
Research Article

Abstract

Natural colourants with bio-potential are of great commercial demands as we are moving away from the hazardous and toxic chemical dyes. Bixa orellana L. a representative of Bixaceae is rich in bixin and nor-bixin pigments which could be explored for various applications. In the present study, extraction and characterization of bixin and its associated pigment from the aril of the B. orellana L. seeds were performed using various spectroscopic techniques. Spectroscopic analysis and Gas chromatographic profiling of the pigment were performed to understand the present pigments. Toxicity was evaluated through in silico method. The major component, bixin and nor-bixin were proved to be non-toxic, non-carcinogenic and non-mutagenic through in silico methods. The pigment was found to be a potent antioxidant as well as bactericidal against opportunistic bacteria. It was found that bixin extract was a potential antioxidant and bactericidal agent. Hence it could be used for imparting bactericidal potential for cellulosic materials like papers or textiles in biomedical applications.

Keywords

Bixin Co-pigments Gas chromatography Mass spectroscopy Stability Natural colourant 

Notes

Acknowledgements

The authors are thankful to Department of Science and Technology, Government of India and TNSCST for providing the grant to facilitate the research [Sanction Order No. DST/SSTP/TN/2K 10/126(G) 13-09-2011]. The authors thank Management and Department of Botany and Microbiology, Lady Doak College, Madurai for providing the facilities and supporting the work.

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

40011_2017_927_MOESM1_ESM.docx (2.1 mb)
Supplementary material 1 (DOCX 2200 kb)

References

  1. 1.
    Sen P (1993) Therapeutic potential of Bixa: from experience to facts. Drug News Views 1:15–21Google Scholar
  2. 2.
    Patnaik S, Mishra SR, Choudhary GB, Panda SK, Behera M (2011) Phytochemical investigation and simultaneously study on anticonvulsant, antidiabetic activity of different leafy extracts of Bixa orellana Linn. IJPBA 2(5):1497-01Google Scholar
  3. 3.
    Yong YK, Zakaria ZA, Kadir AA, Somchit MN, Ee Cheng Lian G, Ahmad Z (2013) Chemical constituents and antihistamine activity of Bixa orellana leaf extract. BMC Compl Altern Med 13:32CrossRefGoogle Scholar
  4. 4.
    Selvi AT, Aravindhan R, Madhan B, Raghava Rao J (2013) Studies on the application of natural dye extract from Bixa orellana seeds for dyeing and finishing of leather. Ind Crops Prod 43:84–86CrossRefGoogle Scholar
  5. 5.
    Chen HY, Yen GC (2007) Antioxidant activity and free radical scavenging capacity of extracts from guava (Psidium guajava L.) leaves. Food Chem 101(2):686–694CrossRefGoogle Scholar
  6. 6.
    Zaretzki J, Matlock M, Swamidass SJ (2013) XenoSite: accurately predicting CYP- mediated sites of metabolism with neural networks. J Chem Inform Model 53(12):3373–3383CrossRefGoogle Scholar
  7. 7.
    Hughes TB, Miller GP, Swamidass SJ (2015) Modeling epoxidation of drug-like molecules with a deep machine learning network. ACS Central Sci 1(4):168–180CrossRefGoogle Scholar
  8. 8.
    Hughes TB, Miller GP, Swamidass SJ (2015) Site of reactivity models predict molecular reactivity of diverse chemicals with glutathione. Chem Res Toxicol 28(4):797–809CrossRefGoogle Scholar
  9. 9.
    Dang NL, Hughes TB., Krishnamurthy V, Swamidass SJ (2016) A simple formula predicts UGT- mediated metabolism. Bioinformatics 32(20):3183–3189CrossRefGoogle Scholar
  10. 10.
    Giridhar P, Venugopalan A, Parimalan R (2014) A review on annatto dye extraction, analysis and processing-a food technology perspectives. J Sci Res Rep 3(2):327–348Google Scholar
  11. 11.
    Bernard K, Grosjean M (1995) Infrared spectroscopy. In: Britton G, Liaaen-Jensen S, Fander P (eds) Carotenoid, spectroscopy, vol 1B. Birkhauser Verlag, Basel, pp 117–134Google Scholar
  12. 12.
    Roy RC, Steven JM, Dale RS, Fu-Hung H, Michael ST (1991) J Food Sci 56(1):80CrossRefGoogle Scholar
  13. 13.
    Galindo-Cuspinera V, Lubran MB, Rankin SA (2002) Comparison of volatile compounds in water and oil soluble annatto (Bixa orellana L.) extracts. J Agric Food Chem 50(7):2010–2015CrossRefGoogle Scholar
  14. 14.
    Hallagan JB, Allen DC, Borzelleca JF (1995) The safety and regulatory status of food, drug and cosmetics color additives exempt from certification. Food Chem Toxicol 33(6):515–528CrossRefGoogle Scholar
  15. 15.
    Gulrajini ML, Gupta D, Ray Maulik S (1999) Studies on dyeing with natural dyes: part I-dyeing of annatto on nylon and polyester. Indian J Fiber Text Res 24(2):131–135Google Scholar
  16. 16.
    Sekar N (2004) Annato colorants. Colourage 51(7):67Google Scholar
  17. 17.
    Smith J (2006) Annato extracts- chemical and technical assessment. In: Chemical and technical assessment manual, pp 1–21Google Scholar
  18. 18.
    Venugopalan A, Gridhar P, Ravishankar GA (2011) Food, ethanobotanical and diversified applications of Bixa orellana L.: a scope for its improvement through biotechnological mediation. Int J Fund Appl Life Sci 1(4):9–31Google Scholar
  19. 19.
    Yolmeh MB, Habibi-Najafi M, Shakouri S, Hosseini F (2015) Comparing antibacterial and antioxidant activity of annatto dye extracted by conventional and ultrasound- assisted methods. Zahedan J Res Med Sci 15:29–33Google Scholar
  20. 20.
    Paumgartten FJR, De Carvalho RR, Araujo IB, Pinto FM, Borges OO, Souza CAM, Kuriyama SN (2002) Evaluation of the developmental toxicity of annatto in the rat. Food Chem Toxicol 40:1595-01CrossRefGoogle Scholar
  21. 21.
    Shilpi JA, Taufiq-Ur-Rahman M, Uddin SJ, Alam MS, Sadhu SK, Seidel V (2006) Preliminary phramacological screening of Bixa orellana L. leaves. J Ethanopharmacol 108:264–271CrossRefGoogle Scholar
  22. 22.
    Moreira PR, Maioli MA, Medeiros HCD, Guelfi M, Pereira FTV (2014) Protective effect of bixin on carbon tetrachloride-induced hepatotoxicity in rats. Biol Res 47:49CrossRefGoogle Scholar
  23. 23.
    Giuliano G, Rosati C, Bramley PM (2003) To dye or not to dye: biochemistry of annatto unveiled. Trends Biotechnol 21:513–516CrossRefGoogle Scholar

Copyright information

© The National Academy of Sciences, India 2017

Authors and Affiliations

  • Vinod Kumar Nathan
    • 1
    • 2
    Email author
  • Mary Esther Rani
    • 1
  • Gunaseeli Rathinasamy
    • 3
  • Kannan Narayanan Dhiraviam
    • 4
  1. 1.Centre for Research, Department of Botany and MicrobiologyLady Doak CollegeMaduraiIndia
  2. 2.National Institute of Oceanography-CSIRCochinIndia
  3. 3.Centre for Environmental StudiesLady Doak CollegeMaduraiIndia
  4. 4.Department of Plant Biotechnology, School of BiotechnologyMadurai Kamaraj UniversityMaduraiIndia

Personalised recommendations