Cloning and Expression Analysis of 4-Coumarate CoA Ligase (4CL) Gene in Sugarcane Clones Varying in Lignin Content

  • Lakshmi KasirajanEmail author
  • Sara Charles
  • Kalaivaani Aruchamy
Research Article


Sugarcane (Saccharum sp.) is one of the highest biomass producing plant, which is the best lignocellulosic feedstock for ethanol production. To achieve a more efficient conversion of biomass to ethanol, a better understanding of the main factors affecting biomass recalcitrance is needed. In the present study, the authors have cloned the partial coding sequence of 4-coumarate-CoA ligase (4CL) from an energy cane (EC 11010). The deduced amino acid sequence of this gene showed 98% similarity with the Zea mays protein and the phylogeny revealed clustering with the same gene from monocots. The quantitative expression of the 4CL gene among the four different sugarcane genotypes (EC 11010, IK 76-81, Co 86032 and CoC 671) showed higher level of transcripts in the energy cane EC 11010.


Lignocellulosic biomass Secondary cell wall Energy canes Real time polymerase chain reaction 



This work has been supported by the Department of Science and Technology, Science Engineering and Research Board project No. SB/EMEQ-001/2013. The authors wish to thank Dr. M. N. Premachandran, Head, Division of Crop Improvement, and Dr. A. Selvi, Principle Scientist, Biotechnology Section and Head, Sugarcane Breeding Institute, Coimbatore for kindly providing sugarcane clones and extending laboratory facility for carrying out the work respectively.

Compliance with Ethical Standards

Conflict of interest

The authors declare that there is no conflict of interests regarding the publication of this paper.


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Copyright information

© The National Academy of Sciences, India 2017

Authors and Affiliations

  • Lakshmi Kasirajan
    • 1
    Email author
  • Sara Charles
    • 1
    • 2
  • Kalaivaani Aruchamy
    • 1
  1. 1.Genomics Laboratory, Division of Crop ImprovementICAR-Sugarcane Breeding InstituteCoimbatoreIndia
  2. 2.Karunya UniversityCoimbatoreIndia

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