Dynamics of Amino Acid Profile of Musca domestica Larva During Cultivation on Substrate Enriched with Microelements
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Advantage in the application of insects as a source of valuable protein relates to obtaining the biologically active substances and environment conservation. Therefore, the subject of the optimal utilization and cultivation of insects is on the interest currently, and the work deals with the investigation of Musca domestica larva cultivation on different substrates with the goal to obtain highly digestible protein with value addition. The optimal concentration of selenium and cobalt in obtaining the biomass of larva with improved amino acid (AA) composition was studied. AA profiles of larva during cultivation on different substrates were investigated within 48, 72 and 96 h using the system using the system of capillary electrophoresis. Kjeldahl analysis was used to determine the crude protein content of larva depending on the concentration of microelements in the substrate. The study indicates that concentration of crude protein, weight and AAs of maggot meal depends on the concentration and combination of selenium and cobalt in the substrate for cultivation. The maximum concentration of crude protein was found to be maximum after the cultivation within 72 h in the substrate enriched with Se and Co (15 mg/kg). However, the less effective but still positive effect was obtained in a cultivation media with 5 mg/kg of Se and Co after 72 h. The most significant effect in the improvement of AA profile of larva was observed at the concentrations of Se + Co 15 mg/kg in cultivation substrate.
KeywordsMusca domestica larva Alternative protein Amino acid profile Crude protein House fly
The work was supported by the Development of Small Forms of Business under Grant 9022ГУ/2015.
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Conflict of interest
The authors do not have any conflict of interest to declare.
- 5.Makkar HPS, Tran G, ValérieHeuzé, Ankers Ph (2014) Current status on use of insects as animal feed. In: Proceedings of the conference of insects to feed the world, Wageningen, The Netherlands, p 61, 14–17 May 2014Google Scholar
- 6.Smith R, Pryor RE (2014) PROteINSECT—do European citizens accept the use of insects for animal feed and human food? In: Proceedings of the conference of insects to feed the world, Wageningen, The Netherlands, p 31, 14–17 May 2014Google Scholar
- 11.Aniebo AO, Erondu ES, Owen OJ (2008) Proximate composition of housefly larvae (Musca domestica) meal generated from mixture of cattle blood and wheat bran. Livest Res Rural Dev 20:12Google Scholar
- 13.Adesulu EA, Mustapha AK (2000) Use of housefly maggots as a fishmeal replaces in tilapia culture: a recent vogue in Nigeria. In: Ftzimmons K, Filho JC (eds) Proceedings of the fifth international symposium on tilapia aquaculture, Rio de Janeiro, Brazil (BRA), pp 138–143, 3–7 Sept 2000Google Scholar
- 17.Fischer CH, Heckmann LHL, Nordentoft S, Hald B, Bjerrum L (2014) Efficiency and scalability in producing feed from manure using the common housefly. In: Proceedings of the conference of insects to feed the world, Wageningen, The Netherlands, p 76, 14–17 May 2014Google Scholar
- 24.Taylor A, Marks V (1978) Cobalt: a review. J Hum Nutr 32:145–177Google Scholar
- 25.Nieboer E, Sanford WE (1985) Essential, toxic and therapeutic functions of metals (including determinant of reactivity). Rev Biochem Toxicol 7:205–245Google Scholar
- 26.Oldfield JO (1999) Selenium. World Atlas, BelgiumGoogle Scholar
- 27.Tian JZ, Yun MS, Ju WS, Long HF, Kim JH, Kil DY, Chang JS, Cho SB, Kim YY, Han IK (2006) Effects of dietary selenium supplementation on growth performance, selenium retention in tissues and nutrient digestibility in growing-finishing pigs. Asian-Australas J Anim Sci 19(1):55–60CrossRefGoogle Scholar