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Optimal Conditions for the Treatment of Shrimp Culture Effluent Using Immobilized Marine Microalga Picochlorum maculatum (PSDK01)

  • S. Dinesh Kumar
  • P. Santhanam
  • P. Prabhavathi
  • B. Kanimozhi
  • M. Abirami
  • Min S. Park
  • Mi-Kyung Kim
Research Article

Abstract

A significant environmental concern has been raised over the wastewater produced from aquaculture including shrimp farms. In order to evaluate the potential of microalgae to treat the wastewater from a shrimp aquaculture, response surface methodology (RSM) was applied to identify optimal conditions for various parameters. Picochlorum maculatum immobilized beads were used to remove excessive nutrients (phosphate, nitrate, nitrite and ammonia) from a 90 days old shrimp (Litopenaeus vannamei) cultured wastewater. The effects of number of algal cells per bead, density of beads per given volume of wastewater, pH, and retention time were investigated. A significant maximum nutrient removal was obtained at pH 7, 24 h of retention time, 150 beads of density and 111,200 cells/ml of algal cell concentration. The primary experimental results were used to RSM for optimizing the variables statistically for maximum nutrient removal. A ‘minimum run resolution V’ central composite design with four variables (pH and retention time, different bead density and algal cell concentrations in beads) was applied to optimize the process. The results showed good fits with the proposed statistical model for the removal of nutrients.

Keywords

Wastewater treatment Shrimp culture Beads Immobilization Picochlorum maculatum Response surface methodology 

Notes

Acknowledgements

The authors are thankful to the Head, Department of Marine Science and authorities of Bharathidasan University for the facilities provided. They (SDK, PS) are indebted to Department of Biotechnology, Government of India for microalgae culture facility provided through extramural project (BT/PR 5856/AAQ/3/598/2012). One of the authors (SDK) thanks the DBT, Govt. of India for Junior Research Fellowship.

Compliance with Ethical Standards

Conflict of interest

There is no conflict of interest among the authors for publishing this manuscript.

Supplementary material

40011_2017_855_MOESM1_ESM.docx (1.1 mb)
Supplementary material 1 (DOCX 1084 kb)

References

  1. 1.
    Wang X, Wang S, Ma Y (2005) Anoxic biological phosphorus removal and effect of excessive aeration on biological phosphorus removal in A2O process. J Chem Indus Eng (China) 56:1565–1570Google Scholar
  2. 2.
    Zhang Z, Zhou J, Wang J, Guo H, Tong J (2006) Integration of nitrification and denitrifying dephosphatation in airlift loop sequencing batch biofilm reactor. Process Biochem 41:599–608CrossRefGoogle Scholar
  3. 3.
    Abdel Hameed MS, Hammouda O (2007) Review: biotechnological potential uses of immobilized algae. Int J Agric Biol 9:183–192Google Scholar
  4. 4.
    Dinesh Kumar S, Santhanam P, Jayalakshmi T, Nandakumar R, Ananth S, Shenbaga Devi A, BalajiPrasath B (2013) Optimization of pH and retention time on the removal of nutrients and heavy metal (zinc) using immobilized marine microalga Chlorella marina. J Biol Sci 13:400–405CrossRefGoogle Scholar
  5. 5.
    Dinesh Kumar S, Santhanam P, Nandakumar R; Ananth S, Nithya P, Dhanalakshmi B, Mi-Kyung Kim (2016) Bioremediation of shrimp (Litopenaeus vannamei) cultured effluent using copepod (Oithona rigida) and microalgae (Picochlorum maculatam and Amphora sp.)—An integrated approach, Desal Wat Treat doi:  10.1080/19443994.2016.1163509
  6. 6.
    Tam NFY, Wong YS (2000) Effect of immobilized microalgal bead concentrations on wastewater nutrient removal. Environ Poll 107:145–151CrossRefGoogle Scholar
  7. 7.
    Adam S, Suresh Kumar P, Santhanam P, Dinesh Kumar S, Prabhavathi P (2015) Bioremediation of tannery wastewater using immobilized marine microalga Tetraselmis sp., experimental studies and pseudo-second order kinetics. J Mar Biol Oceanogr 4:2. doi: 10.4172/2324-8661.1000141 CrossRefGoogle Scholar
  8. 8.
    Dinesh Kumar S, Santhanam P, Lewis-Oscar F, Thajuddin N (2014) A dual role of marine microalga Chlorella sp. (PSDK01) in aquaculture effluent with emphasis on initial population density. Arab J Sci Eng 40:29–35CrossRefGoogle Scholar
  9. 9.
    Abdel Hameed MS (2002) Effect of immobilization on growth and photosynthesis of the green alga Chlorella vulgaris and its efficiency in heavy metals removal. Bull Fac Sci Assiut Uni 31:233–240Google Scholar
  10. 10.
    Ahmadi M, Vahabzadeh F, Bonakdarpour B, Mehranian M, Mofarrah E (2006) Phenolic removal in olive oil mill wastewater using loofah-immobilized Phanerochaete chrysosporium. World J Microb Biot 22:119–127CrossRefGoogle Scholar
  11. 11.
    Yang K, Yu Y, Hwang S (2003) Selective optimization in thermophilic acidogenesis of cheese-whey wastewater to acetic and butyric acids: partial acidification and methanation. Water Res 37:2467–2477CrossRefPubMedGoogle Scholar
  12. 12.
    Aghamohammadi N, Aziz HBA, Isa MH, Zinatizade AA (2007) Powdered activated carbon augmented activated sludge process for treatment of semi-aerobic landfill leachate using response surface methodology. Bioresour Technol 98:3570–3578CrossRefPubMedGoogle Scholar
  13. 13.
    Hadavifar M, Zinatizadeh AA, Younesi H, Galehdar M (2010) Fenton and photo-Fenton treatment of distillery effluent and optimization of treatment conditions with response surface methodology. Asia-Pac J Chem Eng 5:454–464CrossRefGoogle Scholar
  14. 14.
    Lau PS, Tam NFY, Wong YS (1997) Wastewater nutrients (N and P) removal by carrageenan and alginate immobilized Chlorella vulgaris. Environ Technol 18:945–951CrossRefGoogle Scholar
  15. 15.
    de-Bashan LE, Antoun H, Bashan Y (2005) Cultivation factors and population size control the uptake of nitrogen by the microalgae Chlorella vulgaris when interacting with the microalgae growth promoting bacterium Azospirillum brasilense. FEMS Microbiol Ecol 54:197–203CrossRefPubMedGoogle Scholar
  16. 16.
    Abdel Hameed MS (2007) Effect of algal density in bead, bead size and bead concentrations on wastewater nutrient removal. Afr J Biotechnol 6:1185–1191Google Scholar
  17. 17.
    Venkatesan R, Kumaraguru Vasagam KP, Balasubramanian T (2006) Culture of marine microalgae in shrimp farm discharge water: a sustainable approach to reduce the cost production and recovery of nutrients. J Fish Aquat Sci 1:262–269CrossRefGoogle Scholar
  18. 18.
    Pereira H, Custódio L, Rodrigues MJ, de Sousa CB, Oliveira M, Barreira L, Abu-Salah KM (2015) Biological activities and chemical composition of methanolic extracts of selected autochthonous microalgae strains from the Red Sea. Mar Drugs 13:3531–3549CrossRefPubMedPubMedCentralGoogle Scholar
  19. 19.
    Jenkins D, Medsker LL (1964) A brucine method for the determination of nitrate in Ocean, Estuarine, and Fresh Waters. Anal Chem 36:610–612CrossRefGoogle Scholar
  20. 20.
    Strickland SC, Parsons TR (1972) A practical handbook of seawater analyses. Bulletin of Fisheries Research Board of Canada, OttawaGoogle Scholar
  21. 21.
    APHA (1998) Standard methods for the examination of water and wastewater. American Public Health Association, WashingtonGoogle Scholar
  22. 22.
    Santos MMD, Moreno-Garrido I, Gonçalves F, Soares Amadeu MVM, Ribeiro R (2002) An in situ bioassay for estuarine environments using the microalga Phaeodactylum tricornutum. Environ Toxicol Chem 21:567–574CrossRefPubMedGoogle Scholar
  23. 23.
    Liang J, Han F, Wang Z, Yao Y, Mo F (2009) Chemical composition of the essential oil from leaves of Callicarpa nudiflora. Chem Nat Comp 45:267–268CrossRefGoogle Scholar
  24. 24.
    Matheickal JT, Yu Q, Woodburn GM (1999) Biosorption of cadmium (II) from aqueous solution by pre-treated biomass of marine algae Durvillaea potatorum. Water Res 33:335–342CrossRefGoogle Scholar
  25. 25.
    Soumya GN, Manickavasagam M, Santhanam P, Dinesh Kumar S, Prabhavathi P (2015) Removal of phosphate and nitrate from aqueous solution using seagrass Cymodocea rotundata beads. Afr J Biotechnol 14:1393–1400CrossRefGoogle Scholar
  26. 26.
    Soumya GN, Manickavasagam M, Santhanam P, Dinesh Kumar S, Vasanthi D, Karuppasamy PK (2015) Optimization of pH, retention time, biomass dosage in beads and beads density on textile dye effluent bioremediation using seagrass, Cymodocea rotundata beads. J Bioremed Biodeg 6:2. doi: 10.4172/2155-6199.1000295 CrossRefGoogle Scholar
  27. 27.
    Pakshirajan K, Singh S (2010) Decolorization of synthetic wastewater containing azo dyes in a batch-operated rotating biological contactor reactor with the immobilized fungus Phanerochaete chrysosporium. Ind Eng Chem Res 49:7484–7487CrossRefGoogle Scholar
  28. 28.
    Hannoun BJ, Stephanopoulos G (1986) Diffusion coefficients of glucose and ethanol in cell-free and cell-occupied calcium alginate membranes. Biotechnol Bioeng 28:829–835CrossRefPubMedGoogle Scholar
  29. 29.
    Robinson PK, Mak AL, Trevan MD (1986) Immobilized algae: a review. Proc Bioch 21:122–127Google Scholar
  30. 30.
    Jimenez-Perez MV, Sanchez-Castillo P, Romera O, Fernandez-Moreno D, Perez-Martinez C (2004) Growth and nutrient removal in free and immobilized planktonic green algae isolated from pig manure. Enz Microb Technol 34:392–398CrossRefGoogle Scholar
  31. 31.
    Dinesh Kumar S, Biodiversity of phytoplankton in Muthukuda mangrove environment, Southeast coast of India and its utilization for aquaculture wastewater remediation and valuable co-product. Ph. D. Thesis, Bharathidasan UniversityGoogle Scholar
  32. 32.
    Lukavsky J, Komarek J, Lukavska A, Ludvik J, Pokorny J (1986) Metabolic activity and cell structure of immobilized algal cells (Chlorella, Scenedesmus). Arch Hydrobiol Suppl 73:261–279Google Scholar

Copyright information

© The National Academy of Sciences, India 2017

Authors and Affiliations

  • S. Dinesh Kumar
    • 1
    • 2
  • P. Santhanam
    • 1
  • P. Prabhavathi
    • 3
  • B. Kanimozhi
    • 4
  • M. Abirami
    • 4
  • Min S. Park
    • 5
  • Mi-Kyung Kim
    • 2
  1. 1.Marine Planktonology and Aquaculture Laboratory, Department of Marine Science, School of Marine SciencesBharathidasan UniversityTiruchirappalliIndia
  2. 2.MCK Biotech Co. LtdDaegu R&D Fusion CenterDaeguSouth Korea
  3. 3.Department of MicrobiologyNadar Saraswathi College of Arts and ScienceTheniIndia
  4. 4.PG and Research Department of MicrobiologyPSG College of Arts and ScienceCoimbatoreIndia
  5. 5.Center for Microalgal Technology and Biofuels, Institute of HydrobiologyChinese Academy of ScienceWuhanChina

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