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Phytochemical Screening and Antifungal Efficacy of Closely Related Senna obtusifolia and Senna tora on Some Phytopathogenic Fungi

  • Sanjay Kumar Mishra
  • Arun Salick Moses
Research Article
  • 53 Downloads

Abstract

The antifungal activity of crude methanolic leaf extract of closely related Senna obtusifolia and Senna tora of family Caesalpiniaceae was evaluated by growth inhibition analysis on four phytopathogenic fungi viz. Alternaria helianthii, Pythium aphanidermatum, Fusarium oxysporum and Aspergillus niger. In spite of morphological similarities, the methanolic leaf extracts of these species show qualitative and quantitative differences in their chemical profiles establishing them as two distinct chemo-types. The methanolic extract of both the species contain two or more antifungal compounds that act synergistically, inhibiting fungal growth and reproduction. Senna obtusifolia have shown better antifungal efficacy as compared to its close relative S. tora on all the fungal phytopathogens investigated. Scanning electron microscopy observations reveal that the crude methanolic extract induced shrinkage and distortion of fungal mycelium adversely affecting the development of reproductive structures. These results can be correlated with synergistic or additive effect imposed by two or more antifungal compounds present in the crude extract. Statistical analysis support the characteristic difference in antifungal efficacy of methanolic extract of these species. This difference may be due to different permutations and combinations of antifungal compounds present in the extract of these closely related species.

Keywords

Antifungal Phytopathogen Cassiinae Senna Synergistic 

Notes

Acknowledgements

The authors are thankful to the University Grants Commission, New Delhi, India for financial support under Minor research project to complete the above research work. They are grateful to Prof. N. K. Dubey, Department of Botany, Banaras Hindu University for constructive suggestions and encouragement. Authors are also thankful to Dr. M. Massey, Principal and Dr. M. Pati, Head, Department of Botany, Ewing Christian College, Allahabad for providing necessary facilities and being source encouragement. Thanks are due to Dr. A.K. Pathak, Department of Physics, E. C .C. for statistical analysis of data used in the above paper.

Compliance with Ethical Standards

Conflict of interest

The authors have no commercial associations that might pose a conflict of interest here with in connection with the submitted article. They also certify that it is an original research work, not published or under consideration for publication anywhere else.

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Copyright information

© The National Academy of Sciences, India 2017

Authors and Affiliations

  1. 1.Department of BotanyEwing Christian CollegeAllahabadIndia

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