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In Vitro Plant Regeneration by Organogenesis from Leaf Callus of Carnation, Dianthus caryophyllus L. cv. ‘Master’

  • Kalpna Thakur
  • Kamlesh Kanwar
Research Article
  • 134 Downloads

Abstract

An efficient indirect in vitro plant regeneration protocol for Dianthus caryophyllus L. cv. ‘Master’ was developed using leaf explants. This study revealed the morphogenetic potential of leaf explant as a source for micropropagation. Murashige and Skoog (MS) medium supplemented with 2.0 mg/l 2,4-dichlorophenoxyacetic acid and 0.5 mg/l Naphthalene acetic acid (NAA) resulted in maximum (94.44%) callus induction. MS medium supplemented with 1.5 mg/l thidiazuron, 0.25 mg/l Kinetin and 0.25 mg/l NAA was found to be highest for average shoot regeneration (80.56%), average number of shoots (6.01) and average shoot length (1.93 cm). For in vitro multiplication of shoots, MS medium supplemented with 2.0 mg/l Kinetin and 0.25 mg/l NAA was found to be the best which resulted in 14.64 average number of microshoots. It was observed that the half strength MS basal medium supplemented with 1.5 mg/l Indole-3-butyric acid and 0.02% activated charcoal showed maximum rooting (98.19%) with 9.60 average number of roots per microshoot having 4.24 cm root length. The in vitro rooted plantlets were hardened gradually and were successfully acclimatized under ex vitro conditions. The genetic variation in the in vitro raised plants was confirmed using DNA based markers [Random Amplified Polymorphic DNA and Inter Simple Sequence Repeats] for assessment of genetic stability of plants raised through indirect regeneration.

Keywords

In vitro Indirect regeneration Dianthus caryophyllus Hardening DNA based markers 

Notes

Acknowledegment

The authors would like to thank Dr. Y. S. Parmar University of Horticulture and Forestry, Solan, Himachal Pradesh, India for providing laboratory and field facilities.

Compliance with Ethical Standards

Conflict of interest

Both authors declare no conflict of interest.

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Copyright information

© The National Academy of Sciences, India 2017

Authors and Affiliations

  1. 1.Department of BiotechnologyDr. Y.S. Parmar University of Horticulture and ForestrySolanIndia

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