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In Vitro Culture of Rheum emodi Wall: An Endangered Medicinal Plant of Northwestern Himalaya

  • Shagoon Tabin
  • Azra N. Kamili
  • R. C. Gupta
  • Javid A. Parray
  • Anshu Bansal
Research Article
  • 75 Downloads

Abstract

Rheum emodi Wall is a well-known medicinal plant found on high altitudes. The plant is under tremendous anthropogenic pressure due to its over exploitation. In this context, biotechnological intervention particularly tissue culture is the need of hours for its conservation. In the present study, a successful and reproducible protocol was standardized for the micropropagation of various R. emodi using various explants and treatments. The explants used were seeds, leaves, shoots and rhizomes which were cultured on Murashige and Skoog (MS) medium, supplemented with different concentrations and combinations of phytohormones i.e. 6-benzylaminopurine (BAP), kinetin, indole-3-acetic acid, napthalene acetic acid, indole-3-butyric acid (IBA), 2,4-dichlorophenoxyacetic acid, Zeatin and Thidiazuron. The most effective concentration and combination for the plantlet formation was MS + (15 µM) BAP + (15 µM) IBA.

Keywords

Altitudinal herbs Himalaya Micropropgation Plant growth regulators Rheum 

Notes

Acknowledgements

This study was supported by Department of Science and Technology, Ministry of Science and Technology (sr/wos-A/Ls-236) and DBT GoI, New Delhi funded women entrepreneurship project, the assistance of which is highly acknowledged.

Compliance with Ethical Standards

Conflict of interest

The authors hereby declare that they have no conflict of interest. All authors equally participated in designing experiments analysis and interpretation of data. All authors read and approved the final manuscript.

References

  1. 1.
    Anjen L, Alisa EG, Suk-pyo H, McNeill J, Hideaki O, Park C, Liao M (2003) Polygonum (Polygonaceae). Flora of China 5:278–315Google Scholar
  2. 2.
    Ganie AH, Tali BA, Khuroo AA, Nawchoo IA, Rather AM (2014) Rheum spiciforme Royle (Polygonaceae): a new record to the flora of Kashmir Valley, India. Natl Acad Sci Lett 37:561–565CrossRefGoogle Scholar
  3. 3.
    Phartyal SS, Thapliyal RC, Koedam N, Godefroid S (2002) Ex situ conservation of rare and valuable forest tree species through seed gene bank. Curr Sci 83:1351–1357Google Scholar
  4. 4.
    Malik AH, Khuroo AA, Dar GH, Khan ZS (2011) Ethnomedicinal uses of some plants in the Kashmir Himalaya. Indian J Tradit Knowl 10:362–366Google Scholar
  5. 5.
    S K, Sastry ARK (1980) Threatened Plants of India—a state of the art report, BSI printed by Howrah Mehta Offset Works, New DelhiGoogle Scholar
  6. 6.
    Nautiyal BP, Prakash V, Maithani UC, Chauhan RS, Purohit H, Nautiyal MC (2003) Germinability, productivity and economic viability of Rheum emodi Wall. ex Meissn. cultivated at lower altitude. Curr Sci 84:143–148Google Scholar
  7. 7.
    Chaurasia OP, Ahmed Z, Ballabh B (2007) Ethnobotany and plant of trans-Himalaya. Satish Serial Publishing House, Delhi, pp 1–544Google Scholar
  8. 8.
    Castleman M (1991) The healing herbs: the ultimate guide to the curative powers of nature’s medicine. Rodale Press, Emmaus, pp 305–307Google Scholar
  9. 9.
    Peigen X, Liyi H, Liwei W (1984) Ethnopharmacologic study of Chinese rhubarb. J Ethnopharmacol 10:275–293CrossRefPubMedGoogle Scholar
  10. 10.
    Borgia M, Sepe N, Borgia R, Ori-Bellometti M (1981) Pharmacological activity of an herbal extract: controlled clinical study. Curr Ther Res 29:525–536Google Scholar
  11. 11.
    Kapoor LD (1990) Handbook of ayurvedic medicinal plants. CRC Press, Boca Raton and London, p 487Google Scholar
  12. 12.
    Yen GC, Duh PD, Chuang DY (2000) Antioxidant activity of anthraquinones and anthrone. Food Chem 70:437–441CrossRefGoogle Scholar
  13. 13.
    Cai X, Xu S (2007) Meiosis-driven genome variation in plants. Curr Genom 8:151–161CrossRefGoogle Scholar
  14. 14.
    Semple SJ, Pyke SM, Reynolds GD, Flower RL (2001) In vitro antiviral activity of the anthraquinone chrysophanic acid against poliovirus. Antiviral Res 49:169–178CrossRefPubMedGoogle Scholar
  15. 15.
    Alam MMA, Javed K, Jafri MA (2005) Effect of Rheum emodi (Revand Hindi) renal functions in rats. J Ethnopharmacol 96:121–125CrossRefPubMedGoogle Scholar
  16. 16.
    Kong LD, Cheng CHK, Tan RX (2004) Inhibition of MAO A and B by some plant derived alkaloids, phenols and anthraquinones. J Ethnopharmacol 91:351–355CrossRefPubMedGoogle Scholar
  17. 17.
    Akhtar MS, Amin M, Ahmad M, Alamgeer A (2009) Hepatoprotective effect of Rheum emodi roots (Revand chini) and Akseer-e-Jigar against paracetamol-induced hepatotoxicity in rats. Ethnobot Leafl 13:310–315Google Scholar
  18. 18.
    Ho TY, Wub SL, Chenc JC, Lid CC, Hsiand CY (2007) Emodin blocks the SARS coronavirus spike protein and angiotensin-converting enzyme 2 interaction. Antiviral Res 74:92–101CrossRefPubMedGoogle Scholar
  19. 19.
    Radhika R, Kumari DK, Sudarsanam D (2010) Anti-diabetic activity of R. emodi in Alloxan induced diabetic rats. Int J Pharm Sci Res 1:296–300Google Scholar
  20. 20.
    Kuo PL, Lin TC, Lin CC (2002) The antiproliferative activity of aloe-emodin is through p53-dependent and p21-dependent apoptotic pathway in human hepatoma cell lines. Life Sci 71:1879–1892CrossRefPubMedGoogle Scholar
  21. 21.
    Rajkumar V, Guha G, Kumar RA (2011) Antioxidant and anti-cancer potentials of Rheum emodi rhizome extracts. Evid Based Complement Altern Med. Article ID 697986. doi: 10.1093/ecam/neq048
  22. 22.
    Kala CP (2002) Indigenous knowledge of Bhotiya tribal community on wool dyeing and its present status in the Garhwal Himalaya India. Curr Sci 83:814–817Google Scholar
  23. 23.
    Malik S, Kumar R, Vats SK, Bhushan S, Sharma M, Ahuja PS (2009) Regeneration in Rheum emodi Wall a step towards conservation of an endangered medicinal plant species. Eng Life Sci 9:130–134CrossRefGoogle Scholar
  24. 24.
    Parray JA, Kamili AN, Reshi ZA, Qadri RA, Jan S (2015) Interaction of rhizobacterial strains for growth improvement of Crocus sativus L. under tissue culture conditions. Plant Cell Tissue Organ 121:325–334. doi: 10.1007/s11240-014-0703-1 CrossRefGoogle Scholar
  25. 25.
    Parray JA, Kamili AN, Hamid R, Husaini AM (2012) In vitro cormlet production of saffron (Crocus sativus L. Kashmirianus) and their flowering response under greenhouse. GM Crops Food Biotechnol Agric Food 3(4):289–295CrossRefGoogle Scholar
  26. 26.
    Hamid R, Kamili AN, Zaffar M, da Silva JAT, Mujib A, Parray JA (2010) Callus mediated shoot organogenesis from shoot tips of Cichorium intybus. J Med Aromat Plant Sci Biotechnol 4(Special Issue 1):84–86Google Scholar
  27. 27.
    Parray JA, Kamili AN, Hamid R, Reshi ZA, Qadri RA (2015) Antibacterial and antioxidant activity of methanol extract of stigma and in vitro raised callus of Crocus sativus Kashmirianus c.v. Front Life Sci 8(1):40–46CrossRefGoogle Scholar
  28. 28.
    Rao SR, Ravishankar GA (2002) Plant cell cultures: chemical factories of secondary metabolites. Biotechnol Adv 20:101–153CrossRefPubMedGoogle Scholar
  29. 29.
    Lal N, Ahuja PS (1989) Propagation of Indian rhubarb (Rheum emodi Wall.) using shoot tip and leaf explants culture. Plant Cell Rep 8:439–496CrossRefGoogle Scholar
  30. 30.
    El-Seedi HR, Zayed M, Roshdy S, Salem M, Hawata M, El-Essawy F, El-Barbary M, El-Kousy S (2010) Analysis of the essential oil from the aerial parts of Psoralea pubescence (Miq.) Standl and its antibacterial activity. Med Chem Res 19:1036–1042CrossRefGoogle Scholar
  31. 31.
    El-Seedi HR, Burman R, Mansour A, Turki Z, Boulos L, Borg-Karlson AK, Gullbo J, Goransson U (2013) The traditional medical uses and cytotoxic activities of sixty-one Egyptian plants: discovery of an active cardiac glycoside from Urginea maritime. J Ethnopharmacol 145:746–757CrossRefPubMedGoogle Scholar
  32. 32.
    Hu CY, Wang PJ (1983) Meristem shoot tip and bud culture. In: Evans DA, Sharp WR, Ammirato PV, Yamada Y (eds) Handbook of plant cell culture. Macmillan, New York, pp 177–227Google Scholar
  33. 33.
    Wareing PF, Phillips IDJ (1981) Growth and differentiation in plants, 3rd edn. Butterworth-Heinemann Limited Pergamon, Oxford, p 343Google Scholar
  34. 34.
    Lal NPS (2000) Adventitious shoot bud formation from cultured leaf explants of Rheum emodi wall. Plant Tissue Cult 12:173–180Google Scholar
  35. 35.
    Parveen S, Kamili AN, Shah AM (2012) Impact of BAP and different auxins on in-vitro shoot proliferation of Rheum emodi Wall. J Pharm Biol Sci 4:47–52Google Scholar
  36. 36.
    Lal N, Ahuja PS (1993) Assessment of liquid culture procedures for in vitro propagation of Rheum emodi. Plant Cell Tissue Organ Cult 34:223–226CrossRefGoogle Scholar
  37. 37.
    Tabin S, Kamili AN, Gupta RC (2016) Novel study on in vitro culture of Rheum spiciforme Royle: an endangered medicinal plant of Gurez valley. Int J Curr Res 8(4):1–9CrossRefGoogle Scholar
  38. 38.
    Tabin S, Kamili AN, Gupta RC (2014) In vitro micro propagation of Rheum explants supplemented with various types of growth hormones. J Agric Vet Sci (IOSR-JAVS) 7(3):97–100Google Scholar
  39. 39.
    Walkey DGA, Mathews KA (1979) Rapid clonal propagation of rhubarb (Rheum rhaponiticum L) from meristem tips in tissue culture. Plant Sci Lett 14:287–290CrossRefGoogle Scholar
  40. 40.
    Roggemans J, Boxus P (1988) Rhubarb (Rheum rhaponticum L). In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, 6. Crops II. Springer, Berlin, pp 339–350Google Scholar

Copyright information

© The National Academy of Sciences, India 2017

Authors and Affiliations

  • Shagoon Tabin
    • 1
  • Azra N. Kamili
    • 1
  • R. C. Gupta
    • 2
  • Javid A. Parray
    • 1
  • Anshu Bansal
    • 2
  1. 1.Centre of Research for DevelopmentSrinagarIndia
  2. 2.Department of BotanyPunjabi University PatialaIndia

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