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Enhanced Production of Nitrilase from Streptomyces sp. MTCC 7546 by Response Surface Method

  • Astha Agarwal
  • Vinod Kumar Nigam
Research Article
  • 143 Downloads

Abstract

Nitrilases are enzymes with varied applications preferably used in preparation of industrially important compounds as well as in bioremediation processes. The production of enzymes is influenced by medium compositions as some of the constituents of medium directly affect the enzyme synthesis. In the present study, the components of the medium are optimized by classical and response surface methodology to maximize the production of intracellular nitrilase from Streptomyces sp. MTCC 7546. Glucose was observed as the most suitable carbon source for production of nitrilase by ‘one factor at a time’. The optimal concentration of glucose (5 g/L) was set as zero coded level for further optimization study. A central composite design was employed to study the effect of interaction among glucose, yeast extract and mineral base and evaluated for maximum nitrilase yield. The nitrilase production of 11.54 U/mg was observed at 24 h in medium containing 6.5 g/L glucose, 1.0 g/L yeast extract and 220 mL/L mineral base with 10 mM of benzonitrile as inducer. A twofold increment from 5.95 U/mg in the enzyme yield was observed after the optimization study of response surface methodology.

Keywords

Indole-3-acetic acid Indole-3-acetonitrile Nitrilase Optimization Streptomyces 

Notes

Acknowledgments

Authors are thankful to the University Grants Commission, New Delhi, India for providing Junior Research Fellowship to Astha Agarwal (F.2-29/2011; SA-1) for carrying out this research work. Authors are also thankful to Centre of Excellence (COE), Department of Biotechnology under TEQIP phase II (Ref No: NPIU/TEQIP II/FIN/31/158; Dated 16th April 2013) for laboratory facilities and infrastructure support and lastly, authors are grateful to Dr. Alok Varshney for sincere help during experimental work. Authors also declare that there is no financial or commercial conflict of interest.

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Copyright information

© The National Academy of Sciences, India 2015

Authors and Affiliations

  1. 1.Department of Bio-EngineeringBirla Institute of TechnologyRanchiIndia

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