Effect of AM Fungi (Gf, Gm) on Biomass and Gymnemic Acid Content of Gymnema sylvestre (Retz.) R. Br. ex Sm.

  • Saurabha B. Zimare
  • Mahesh Y. Borde
  • Paramjeet Kaur Jite
  • Nutan P. MalpathakEmail author
Research Article


In the present investigation, effect of AM fungi (Glomus fasciculatum and Glomus mosseae) on G. sylvestre growth and gymnemic acid production was studied. Pure cultures of G. fasciculatum and G. mosseae were used as a mycorrhizal inoculum. Higher shoot and root length (32.70 and 34.70 cm) and fresh and dry weight (3.33 and 0.92 g) were recorded in Gf treated plants. HPTLC analysis revealed that Gf treated G. sylvestre showed higher gymnemic acid content in leaves (34.91 mg/g DW), shoot (31.98 mg/g DW) and root (18.72 mg/g DW). As compared to control, Gf treated G. sylvestre showed 2.28 % increase and Gm treated G. sylvestre showed 1.09 % increase in gymnemic acid content for leaf analysis; Gf showed 46 % increase and Gm showed 1.73 % increase for shoot analysis and Gf showed 27 % increase and Gm showed 0.61 % increase for root analysis. As compared to control, Gf and Gm treated G. sylvestre showed 2.28 % increase and 1.09 % increase in gymnemic acid content for leaf analysis; 46 % increase and 1.73 % increase for shoot analysis and 27 % increase and 0.61 % increase for root analysis respectively. AM have positive effect on biomass and gymnemic acid production.


Gymnema sylvestre Glomus mosseae Glomus fasciculatum Gymnemic acid HPTLC Gymnemagenin 



Authors would like to acknowledge DST-PURSE, DRS-SAP III for their financial assistance.


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Copyright information

© The National Academy of Sciences, India 2013

Authors and Affiliations

  • Saurabha B. Zimare
    • 1
  • Mahesh Y. Borde
    • 1
  • Paramjeet Kaur Jite
    • 1
  • Nutan P. Malpathak
    • 1
    Email author
  1. 1.Department of BotanyUniversity of PunePuneIndia

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