Effect of AM Fungi (Gf, Gm) on Biomass and Gymnemic Acid Content of Gymnema sylvestre (Retz.) R. Br. ex Sm.
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In the present investigation, effect of AM fungi (Glomus fasciculatum and Glomus mosseae) on G. sylvestre growth and gymnemic acid production was studied. Pure cultures of G. fasciculatum and G. mosseae were used as a mycorrhizal inoculum. Higher shoot and root length (32.70 and 34.70 cm) and fresh and dry weight (3.33 and 0.92 g) were recorded in Gf treated plants. HPTLC analysis revealed that Gf treated G. sylvestre showed higher gymnemic acid content in leaves (34.91 mg/g DW), shoot (31.98 mg/g DW) and root (18.72 mg/g DW). As compared to control, Gf treated G. sylvestre showed 2.28 % increase and Gm treated G. sylvestre showed 1.09 % increase in gymnemic acid content for leaf analysis; Gf showed 46 % increase and Gm showed 1.73 % increase for shoot analysis and Gf showed 27 % increase and Gm showed 0.61 % increase for root analysis. As compared to control, Gf and Gm treated G. sylvestre showed 2.28 % increase and 1.09 % increase in gymnemic acid content for leaf analysis; 46 % increase and 1.73 % increase for shoot analysis and 27 % increase and 0.61 % increase for root analysis respectively. AM have positive effect on biomass and gymnemic acid production.
KeywordsGymnema sylvestre Glomus mosseae Glomus fasciculatum Gymnemic acid HPTLC Gymnemagenin
Authors would like to acknowledge DST-PURSE, DRS-SAP III for their financial assistance.
- 4.Madhurima, Ansari SH, Prawez A, Sayeed A, Akhtar MS (2009) Pharmacognostic and phytochemical analysis of Gymnema sylvestre R. Br. leaves. J Herb Med Toxicol 3(1):73–80Google Scholar
- 8.Malik JK, Manvi FV, Alagawadi KR, Noolvi M (2008) Evalution of anti-inflammatory activity of Gymnema sylvestre leaves extract in Rats. Int J Green Pharm 2:114–115Google Scholar
- 9.Trivedi PD, Pundarikakshudu KA (2008) Validated high performance thin layer chromatographic method for the estimation of gymnemic acids through gymnemagenin in Gymnema sylvestre, materials, extracts and formulations. IJASE 6:1–19Google Scholar
- 12.Selvaraj T, Mathan N, Rajeshkumar S (2009) Effect of indigenous arbuscular mycorrhizal fungi on some growth parameters and phytochemical constituents of Pogostemon patchouli Pellet. Maejo Int J Sci Technol 3(01):222–234Google Scholar
- 20.Abu-Zeyad R, Khan C, Khoo C (1999) Occurrence of arbuscular mycorrhiza in Castanospermum austral A. Cunn. & C. fraser and effects on growth and production of castanospermine. Mycorrhiza 9:111–117Google Scholar
- 21.Rojas-Andrade R, Cerda-Garcia-Rojas CM, Frias-Hernández JT, Dendooven L, Olalde- Portugal V, Ramos- Valdivia AC (2003) Changes in the concentration of trigonelline in a semi-arid leguminous plant (Prosopis laevigata) induced by an arbuscular mycorrhizal fungus during the presymbiotic phase. Mycorrhiza 13:49–52PubMedCrossRefGoogle Scholar
- 27.Ledig FT, Perry TO (1966) Physiological genetics of the shoot–root ratio. In: Proceedings of the Society of American Foresters. Society of American Foresters, Washington DC, pp 39–43Google Scholar
- 29.Smith SE, Read DJ (2008) Mycorrhizal symbiosis. Elsevier Science, New York 605Google Scholar
- 30.Cox G, Sanders F, Wild J (1995) Ultra structural evidence relating to host-endophyte transfer in vesicular-arbuscular mycorrhiza. In: Sander FE, Mosse B, Tinker PB (eds) Endomycorrhizas. Academic Press, New York, pp 297–312Google Scholar