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Design and Construction of a Line-Confocal Raman Microscope for Sensitive Molecules

  • Barun Kumar Maity
  • Anirban Das
  • Sayan Dutta
  • Sudipta Maiti
Research Article
  • 80 Downloads

Abstract

Surface enhanced Raman spectroscopy (SERS) has great potential for investigating the secondary structure of biomolecules. While enhancements of many orders of magnitude are possible in SERS, the excitation powers required for adequate signal to noise may still be damaging for sensitive protein molecules. Here, we describe the design and construction of a line-confocal Raman micro-spectrometer, which allows investigations at much lower powers compared to a commercial Raman microscope, which uses a point-confocal design. Our design combines a commercial Raman spectrometer and a fluorescence microscope, and achieves simultaneous spectrometry over 400 pixels. This can be used for multiplexed probing of a homogeneous sample, using much lower power for each pixel at comparable signal-to-noise ratios.

Keywords

SERS Raman imaging Low power Raman Protein 

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Copyright information

© The National Academy of Sciences, India 2018

Authors and Affiliations

  • Barun Kumar Maity
    • 1
  • Anirban Das
    • 1
  • Sayan Dutta
    • 1
    • 2
  • Sudipta Maiti
    • 1
  1. 1.Department of Chemical SciencesTata Institute of Fundamental ResearchMumbaiIndia
  2. 2.College of PharmacyPurdue UniversityWest LafayetteUSA

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