Improvement in Seed Germination Through Pre-treatments in Timur (Zanthoxylum armatum DC.): A Plant with High Medicinal, Economical and Ecological Importance

  • Vikas Yadav PatadeEmail author
  • Kundan Kumar
  • Anand Kumar Gupta
  • Atul Grover
  • Prem Singh Negi
  • Sanjai Kumar Dwivedi
Short Communication


Timur (Zanthoxylum armatum DC.) also known as toothache tree or winged prickly ash is an important large thorny wild shrub of Rutaceae family. Owing to its high medicinal properties, the plant population is rapidly depleting due to the unsustainable premature harvesting from open-access forests. Because of which the plant species can become endangered or rare in the absence of conservation measures through its mass propagation. Seed propagation in Timur is a time consuming and tedious process due to the requirement for stratification. Here, we report the effect of different seed pre-treatments including water soaking, potassium nitrate (300 mM), sodium chloride (75 mM) and concentrated sulfuric acid treatments followed by water soaking on seed germination in Timur. Seed pre-treatment with acid for 120 min resulted in rapid and synchronous seed germination whereas the control, water-soaked, potassium nitrate or sodium chloride-treated seeds failed to germinate during the observation period of 30 days. The plantlets from acid-treated seeds further established successfully. Thus, the results of the present study may be applicable in large-scale simple and low-cost mass propagation of the plant as a source of medicines and other purposes.


Timur Dormancy Seed pre-treatment Germination 



Funding was provided by the Defence Research and Development Organisation (Grant No. DIB-77).


  1. 1.
    Brijwal L, Pandey A, Tamta S (2013) An overview on phytomedicinal approaches of Zanthoxylum armatum DC.: an important magical medicinal plant. J Med Plants Res 7(8):366–370Google Scholar
  2. 2.
    Bharti S, Bhushan B (2015) Phytochemical and pharmacological activities of Zantoxylum armatum DC.: an overview. Res J Pharma Biol Chem Sci 6(5):1403–1409Google Scholar
  3. 3.
    Kala CP (2010) Assessment of availability and patterns in collection of Timroo (Zanthoxylum armatum DC.): a case study of Uttarakhand Himalaya. Med Plants - Intl J Phytomed Related Ind 2(2):91–96CrossRefGoogle Scholar
  4. 4.
    Purohit S, Bhatt A, Bhatt ID, Nandi SK (2016) Propagation through air layering in Zanthoxylum armatum DC: an endangered medicinal plant in the Himalayan region. Proc Natl Acad Sci India Sect B Biol Sci 86:607–610CrossRefGoogle Scholar
  5. 5.
    Patade VY, Bhargava S, Suprasanna P (2009) Halopriming imparts tolerance to salt and PEG induced drought stress in sugarcane. Agri Ecosyst Environ 134:24–28CrossRefGoogle Scholar
  6. 6.
    Purohit S, Nandi SK, Palni LMS, Giri L, Bhatt A (2015) Effect of sulfuric acid treatment on breaking of seed dormancy and subsequent seedling establishment in Zanthoxylum armatum DC: an endangered medicinal plant of the Himalayan region. Natl Acad Sci Lett 38(4):301–304CrossRefGoogle Scholar
  7. 7.
    Daudi P, Bisht KS, Pandey V (2016) Propagation techniques of Zanthoxylum alatum Roxb. (a Himalayan toothache shrub). Curr Sci 110(1):30–33Google Scholar

Copyright information

© The National Academy of Sciences, India 2019

Authors and Affiliations

  1. 1.Defence Institute of Bio-Energy Research Field StationPithoragarhIndia
  2. 2.Defence Institute of Bio-Energy ResearchHaldwani, NainitalIndia

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