The Detection Limit of PCR Amplification for Cryptosporidium spp. Oocysts in Fecal Samples

  • Harith Saeed Al-WaridEmail author
  • Ihsan M. Al-Saqur
  • Souhaila H. Mahmood
Short Communication


Detection limits of PCR to detect Cryptosporidium spp. were determined in ninety-six preserved fecal samples (obtained for a different project). Small subunit ribosomal RNA gene was amplified from each sample. The best detection limit of the PCR method was > 150 oocysts.


Cryptosporidium SSU rRNA Conventional PCR Detection limit 



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Copyright information

© The National Academy of Sciences, India 2019

Authors and Affiliations

  • Harith Saeed Al-Warid
    • 1
    • 2
    Email author
  • Ihsan M. Al-Saqur
    • 3
  • Souhaila H. Mahmood
    • 1
  1. 1.Biology Department, College of ScienceUniversity of BaghdadBaghdadIraq
  2. 2.School of Natural ResourcesUniversity of MissouriColumbiaUSA
  3. 3.Al-Israa College UniversityBaghdadIraq

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