The Detection Limit of PCR Amplification for Cryptosporidium spp. Oocysts in Fecal Samples
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Detection limits of PCR to detect Cryptosporidium spp. were determined in ninety-six preserved fecal samples (obtained for a different project). Small subunit ribosomal RNA gene was amplified from each sample. The best detection limit of the PCR method was > 150 oocysts.
KeywordsCryptosporidium SSU rRNA Conventional PCR Detection limit
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