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National Academy Science Letters

, Volume 42, Issue 2, pp 161–167 | Cite as

In Vitro Regeneration and Free Radical Scavenging Assay of Hypericum perforatum L.

  • Mohammad Yaseen MirEmail author
  • Azra N. Kamili
  • Qazi P. Hassan
  • Sabreena Rafi
  • Javid A. Parray
  • Sumira Jan
Short Communication

Abstract

The phytochemistry of Hypericum perforatum (St. John’s wort) has been elaborated extensively owing to its immense application in medicinal chemistry. Most of pharmacological activities were assigned to presence of photosensitive naphthodianthrone, hypericin and other allied flavonoid constituents. Escalating demands of hyperforin as antidepressant added more lure to Hypericum perforatum L. In the current study, efforts were made for quick propagation of the plant by in vitro culture using nodal explants. The nodal explants were cultured on agarized Murashige and Skoog (MS) medium supplemented with auxin and cytokinin. The highest number of shoots (50.5) per explant was found on media supplemented with 6-Benzylaminopurine (BAP) 7.5 μM and Indole-3-acetic acid (IAA) 2.0 µM. Our study showed 1.4 fold increment in shoot number viz –a-viz shoot morphogenesis from the nodal explants took about 24 days which is significantly shorter than earlier published reports. In the current study auxins Indole-3-butyric acid (IBA) and IAA were found to be equally important for root induction, whereas Napthalene acetic acid (NAA) was not required. The maximum root number (15.8) with 100% response was observed on MS + IBA (4.0 µM). Further, free radicals produced due to various environmental stresses and numerous metabolic processes ultimately lead to complete cell impairment. To combat this cellular damage organisms require dynamic defense systems. In addition to inherent defense machinery, we can supplement the defense systems of organisms with natural resources like herbs. In this regard, the free radical scavenging assay was also assessed. In our experiment line, best plant hormonal combination (I1 = MS + BAP 7.5 µM + IAA 2.0 µM) was selected on the basis of higher shootlet formation and their antioxidant activity was compared with in vitro control (I0 = MS) as well as with wild plants (W). I1 plant extracts have shown strong free radical scavenging activity with 1 fold increment as compared with wild and in vitro control.

Keywords

BAP IAA In vitro propagation Free radicals 

Notes

Acknowledgements

The authors would like to acknowledge the Director, CORD, University of Kashmir for providing laboratory facilities.

Compliance with Ethical Standards

Conflict of interest

We wish to confirm that there are no known conflicts of interest associated with this publication and there has been no significant financial support for this work that could have influenced its outcome.

References

  1. 1.
    Victor BG, Wafaa MA, Hamed S (2014) Preliminary phytochemical screening and evaluation of in vitro oxidant activity of Iraqi species of Hypericum perforatum aerial part. Int Res J Pharm 5:369–373CrossRefGoogle Scholar
  2. 2.
    Nurk NM, Crockett SL (2011) Morphological and Phytochemical Diversity among Hypericum Species of the Mediterranean Basin. Med Aromat Plant Sci Biotechnol 5:14–28Google Scholar
  3. 3.
    Patocka J (2003) The chemistry, pharmacology and toxicology of the biologically active constituents of the herb Hypericum perforatum L. J Appl Biomed 1:61–70CrossRefGoogle Scholar
  4. 4.
    Koch MA, Scheriau C, Betzin A, Hohmann N, Sharbel TF (2013) Evolution of cryptic gene pools in Hypericum perforatum: the influence of reproductive system and gene flow. Ann Bot 111:1083–1094CrossRefGoogle Scholar
  5. 5.
    Bruni R, Sacchetti G (2009) Factors affecting polyphenol biosynthesis in wild and field grown St. John’sWort (Hypericum perforatum L. Hypericaceae/Guttiferae). Molecules 14:682–725CrossRefGoogle Scholar
  6. 6.
    Buckley YM, Briese DT, Rees M (2003) Demography and management of the invasive plant species Hypericum perforatum L. Using multi-level mixed-effects models for characterizing growth, survival, and fecundity in a long-term data set. J Appl Ecol 40:481–493CrossRefGoogle Scholar
  7. 7.
    Briese D, Campbell M, Faithfull I (2000) Best practice management guide for environmental weeds. Weeds CRC, Glen OsmondGoogle Scholar
  8. 8.
    Males Z, Brantner AH, Sovic K, Pilepic KH, Plazibat M (2006) Comparative phytochemical and antimicrobial investigations of Hypericum perforatum L. subsp. perforatum and H. perforatum subsp. angustifolium (DC.) Gaudin. Acta Pharm 56:359–367Google Scholar
  9. 9.
    Karioti A, Bilia AR (2010) Hypericins as potential leads for new therapeutics. Int J Mol Sci 11:562–594CrossRefGoogle Scholar
  10. 10.
    Wolfle U, Seelinger G, Christoph M, Schempp M (2014) Topical Application of St. Johnʼs Wort (Hypericum perforatum). Planta Med 80:109–120CrossRefGoogle Scholar
  11. 11.
    Woelk H (2000) Comparison of St John’s wort and imipramine for treating depression: randomised controlled trial. BMJ 321(7260):536–539CrossRefGoogle Scholar
  12. 12.
    Parray JA, Hamid R, Kamili AN, Shameem N, Jan S, Ganai BA (2015) Biological efficacy and radical scavenging potential of shikonin inArnebia benthamii (Wall ex. G Don) Johnston. Ind Crops Prod 74:434–439CrossRefGoogle Scholar
  13. 13.
    Ganie SA, Jan A, Muzaffar S, Zargar BA, Hamid R, Zargar MA (2012) Radi-cal scavenging and antibacterial activity of Arnebia benthamii methanol extract. Asian Pac J Trop Med 5(10):766–772CrossRefGoogle Scholar
  14. 14.
    Hugo WB (1999) Disinfection mechanisms. In: Russel AD, Hugo WB, Ayliffe GAG (eds) Principles and practice of disinfection, preservation and sterilization, 3rd edn. Blackwell Science, Oxford, pp 258–283Google Scholar
  15. 15.
    Palmer DC, Keller WA (2011) Plant regeneration from petal explants of Hypericum perforatum L. Plant Cell Tissue Organ Cult 105:129–134CrossRefGoogle Scholar
  16. 16.
    Wojcik A, Podstolski A (2007) Leaf explant response in in vitro culture of St. John’s wort (Hypericum perforatum L.). Acta Physiol Plant 29:151–156CrossRefGoogle Scholar
  17. 17.
    Reddy CA, Muneppa TS (2014) Influence of additives on enhanced in vitro shoot multiplication of Stevia rebaudiana (Bert.)—an important anti diabetic medicinal plant. Am J Plant Sci 5:192–199CrossRefGoogle Scholar
  18. 18.
    Murch SJ, Krishna RS, Saxena PK (2000) Tryptophan is a precursor for melatonin and serotonin biosynthesis in in vitro regenerated St. John’s wort (Hypericum perforatum L. cv. Anthos) plants. Plant Cell Rep 19:698–704CrossRefGoogle Scholar
  19. 19.
    Franklin G, Dias ACP (2006) Organogenesis and embryogenesis in several Hypericum perforatum genotypes. In Vitro Cell Dev Biol Plant 42:324–330CrossRefGoogle Scholar
  20. 20.
    Mederos MS (1991) In vitro growth and multiplication of Hypericum canariense L. Acta Hortic 289:133–135CrossRefGoogle Scholar
  21. 21.
    Liu SY, Sporer F, Wink M, Jourdane J, Henning R, Li YL, Ruppel A (1997) Anthraquinones in Rheum palmatum and Rumex dentatus (polygonaceae) and phorbol esters in Jatropa curcas (Euphorbiaceae) with molluscicidal activity against the schistosome vector snails oncomelania, Biomphalaria and Bulinus. Trop Med Int Health 2(2):179–188CrossRefGoogle Scholar
  22. 22.
    Padmaja M, Sravanthi M, Hemalatha KPJ (2011) Evaluation of antioxidant activity of two Indian medicinal plants. J Phytol 3(3):86–91Google Scholar
  23. 23.
    Soobrattee MA, Bahorun T, Neergheen VS, Googoolye K, Aruoma OI (2008) Assessment of the content of phenolics and antioxidant actions of the Rubiaceae, Ebenaceae, Celastraceae, Erythroxylaceae and Sterculaceae families of Mauritianendemic plants. Toxicol In Vitro 22:45–56CrossRefGoogle Scholar
  24. 24.
    Pretto FR, Santarem ER (2000) Callus formation and plant regeneration from Hypericum perforatum leaves. Plant Cell Tissue Organ Cult 62:107–113CrossRefGoogle Scholar
  25. 25.
    Gadzovska S, Maury S, Ounnar S, Righezza M, Kascakova S, Refregiers M, Spasenoski M, Joseph C, Hagège D (2005) Identification and quantification of hypericin and pseudohypericin in different Hypericum perforatum L. in vitro cultures. Plant Physiol Biochem 43:591–601CrossRefGoogle Scholar
  26. 26.
    Huang WS, Lee AR, Yang CH (2006) Antioxidative and anti-inflammatoryactivities of polyhydroxy flavonoids of Scutellaria baicalensis Georgi. Biosci Biotechnol Biochem 70:2371–2380CrossRefGoogle Scholar
  27. 27.
    Kirakosyan A, Hayashi H, Inoue K, Charchoglyan A, Vardapetyan H (2000) Stimulation of the production of hypericins by mannan in Hypericum perforatum shoot cultures. Phytochemistry 53:345–348CrossRefGoogle Scholar
  28. 28.
    Rekka E, Kourounakis PN (1991) Effect of hydroxyl ethyl rutenosides and relatedcompounds on lipid peroxidation and free radical scavenging activity. J Pharm Pharmacol 43:486–491CrossRefGoogle Scholar
  29. 29.
    Pirbalouti AG, Azizi S, Koohpayeh A, Golparvar A (2011) In: International conference on bioscience, biochemistry and bioinformatics, Researches Centre of Medicinal Plants and Ethnoveterinary, Islamic Azad University-Shahrekord, Iran, p 5Google Scholar
  30. 30.
    Radulović N, Stankov-Jovanović V, Stojanović G, Šmelcerović A, Spiteller M, Asakawa Y (2007) Screening of in vitro antimicrobial and antioxidant activity of nine Hypericum species from thenBalkans. Food Chem 103:15–21CrossRefGoogle Scholar
  31. 31.
    Sagratini G, Ricciutelli M, Vittori S, Öztürk N, Öztürk Y, Maggi F (2008) Phytochemical and antioxidant analysis of eight Hypericum taxa from Central Italy. Fitoterapia 79:210–213CrossRefGoogle Scholar

Copyright information

© The National Academy of Sciences, India 2018

Authors and Affiliations

  • Mohammad Yaseen Mir
    • 1
    Email author
  • Azra N. Kamili
    • 1
  • Qazi P. Hassan
    • 2
  • Sabreena Rafi
    • 1
  • Javid A. Parray
    • 4
  • Sumira Jan
    • 3
  1. 1.P.G Department of Environmental Science/Centre of Research for DevelopmentUniversity of KashmirSrinagarIndia
  2. 2.Indian Institute of Integrative Medicine (CSIR)Sanat Nagar, SrinagarIndia
  3. 3.ICAR-CITHRangreth, SrinagarIndia
  4. 4.Department of Environmental Science BudgamGovt SAM Degree CollegeBudgamIndia

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