National Academy Science Letters

, Volume 41, Issue 5, pp 285–287 | Cite as

Assessment of Physicochemical Characteristics and Thermophilic Bacterial Enumeration from Hot Water Springs of Himachal Pradesh

  • Poonam Shirkot
  • Ambika VermaEmail author
  • Monika Gupta
  • Anshul Sharma Manjul
  • Ruchika Sharma
Short Communication


Hot water springs are the geothermally heated and each thermal spring is found to be unique and different with respect to thermophilic bacterial diversity and various characteristics such as temperature, pH and chemistry of water. In the present study, pH and temperature of the six thermal springs assessed was found to vary from 6.5 to 8.4 and 36.5 to 94.6 °C respectively. Various physicochemical properties of these water samples evaluated were chloride (609.667–1857.667 mg/l), sulphate (12.667–44.667 mg/l), total hardness (34.833–131.600 mg/l), calcium hardness (29.00–125.00 mg/l), calcium carbonate hardness (58.00–272.33 mg/l) and magnesium content (3.85–26.15 mg/l). Evaluation of these six hot water springs was also carried out and thermophilic bacterial population was found to be highest (3.54 × 105 cfu/g) at Vashisht and minimum (0.54 × 105 cfu/g) at Kalath. In the present strudy most of the thermophilic bacterial isolates were found to belong to families Bacillaceae and Thermaceae.


Physicochemical analysis Enumeration Thermophilic bacterial population 



The author acknowledges the financial assistance received from Department of Biotechnology at Dr YS Parmar University of Horticulture and Forestry, Nauni, Solan, Himachal Pradesh to carry out the whole part of this work.


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Copyright information

© The National Academy of Sciences, India 2018

Authors and Affiliations

  • Poonam Shirkot
    • 1
  • Ambika Verma
    • 1
    Email author
  • Monika Gupta
    • 1
  • Anshul Sharma Manjul
    • 1
  • Ruchika Sharma
    • 1
  1. 1.Department of BiotechnologyDr. Y. S, Parmar University of Horticulture and ForestryNauni, SolanIndia

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