Optimization and Standardization of Conditions for Production of Commercially Viable Formulation of Native Agrobacterium sp. UHFBA-218
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Studies were carried out for the mass multiplication of native Agrobacterium sp. UHFBA-218 (MCC 2101, NCBI: KC488176; deposited as UHFBA-218[Cherry 2E-2-2]) and Rhizobium rhizogenes strain K84 at different pH, temperature, incubation period, broth media and at different air supply. The most effective pH, temperature, incubation period, broth medium and air supply for both the isolates was 7.0, 25 °C, 4 days, yeast extract mannitol broth medium and 20 L/min, respectively. These parameters were further used for mass multiplication of these strains and mixing the multiplied bacterial inoculum for studying the viability of Agrobacterium sp. UHFBA-218 and R. rhizogenes strain K84 in different carrier media—white stone powder, carboxymethyl cellulose and activated charcoal during 6 months of storage at room temperature at three different locations, mixed in one part of broth culture in exponential phase having 270 ± 10 × 1012 cfu/ml to two part of carrier and one part of broth culture mixed to five part of carrier and were compared for viable counts observed in different formulations kept at 4 °C for reference. White stone powder was superior to other carrier media tested under these studies. There were appreciable counts ranging from 34.67 to 103.3 × 108 cfu/g after 6 months of room temperature storage at different locations. However, irrespective of strains, storage at 4 °C temperature provided maximum viability of 108.50 and 155.7 × 108 cfu/g in white stone powder mixed in 1:5 and 1:2 ratios, respectively.
KeywordsAgrobacterium sp. UHFBA-218 Bioformulation Carrier media Viability
The authors are thankful to the Dean, College of Horticulture, Dr. Y. S. Parmar University of Horticulture and Forestry, Nauni, Solan-173 230, Himachal Pradesh for providing lab facilities.
- 7.Anonymous (2012) Completion report of NFBSRA/PCN/AP-18/2007-08 project ‘‘Molecular analysis of agrocin producing Agrobacterium radiobacter for biological control of crown gall in stone fruits”. Department of Mycology and Plant Pathology, Dr. Y.S.Parmar University of Horticulture and Forestry, Nauni, Solan, p 38Google Scholar
- 8.Dua A, Sangwan N, Kaur J, Saxena A, Kohli P, Gupta AK, Lal R (2013) Draft genome sequence of Agrobacterium sp. strain UHFBA-218, isolated from rhizosphere soil of crown gall-infected cherry rootstock Colt. Genome Announc 1(3):e00302-13. https://doi.org/10.1128/genomea.00302-13 CrossRefPubMedPubMedCentralGoogle Scholar
- 9.Okereke GU, Okeh O (2007) Survival of cowpea bradyrhizobia in carrier materials and inoculation response in soil. Afr Crop Sci Soc Conf Proc 8:1183–1186Google Scholar
- 11.Gomez KA, Gomez AA (1983) Statistical procedures for agricultural research, 2nd edn. Wiley, New York, pp 357–427Google Scholar
- 13.Moore LW, Canfield M (1996) Biology of Agrobacterium and management of crown gall disease. In: Hall R (ed) Principles and practice of managing soil borne plant pathogens. APS Press, St. Paul, pp 151–191Google Scholar
- 17.Thomson J A (1984) Production and quality control of carrier based inoculants. International crop research institute for semi-arid tropics. http://dspaceIcrisat.ac.in/handle/10731/908