Advertisement

National Academy Science Letters

, Volume 41, Issue 3, pp 165–167 | Cite as

Symbiotic Efficiency of Slow and Fast-Growing Soybean Rhizobia of Central India

  • Vinod Kumar
  • A. K. Rawat
  • D. L. N. Rao
Short Communication
  • 32 Downloads

Abstract

Soybean rhizobia are pre-dominantly slow growing but fast growing strains that evolved from natural populations have been reported globally including from several locations in India but have not been evaluated. The symbiotic effectivity of three slow and three fast-growing rhizobial strains isolated from Vertisols of Central India was evaluated on soybean var. 9752 in sterilized sand microcosms in a green-house. At 40 days growth, there was variation in nodulation parameters and growth of soybean with both types of rhizobia but overall, the slow growers were superior to fast growers with respect to nodule number (56%), nodule dry matter (25%), shoot dry matter (11%) and total nitrogen uptake (22%). The slow-growers (genetic homology to Bradyrhizobium japonicum) fixed ~ 25% more nitrogen than fast-growers with homology to Rhizobium (Agrobacterium) radiobacter. The results have very important implications for strain selection during biofertilizer production to maximize biological nitrogen fixation.

Keywords

Nitrogen fixation Nodulation Symbiosis Vertisols 

Notes

Acknowledgements

VK is grateful to the Department of Science and Technology, Govt. of India for the award of INSPIRE fellowship. The authors thank the Indian Council of Agricultural Research, New Delhi for funding the project under the aegis of the All India Network Project on Soil Biodiversity-Biofertilizers, ICAR-Indian Institute of Soil Science, Bhopal.

Compliance with Ethical Standards

Conflict of interest

The author declare that they have no conflict of interest.

References

  1. 1.
    Keyser HH, Bohlool BB, Hu TS, Weber DF (1982) Fast-growing rhizobia isolated from root nodules of soybean. Science 215(4540):1631–1632ADSCrossRefPubMedGoogle Scholar
  2. 2.
    Hungria M, Chueire LMO, Coca RG, Megias M (2001) Preliminary characterization of fast growing rhizobial strains isolated from soybean nodules in Brazil. Soil Biol Biochem 33:1349–1361CrossRefGoogle Scholar
  3. 3.
    Sharma MP, Srivastava K, Sharma SK (2010) Biochemical characterization and metabolic diversity of soybean rhizobia isolated from Malwa region of central India. Plant Soil Environ 56:375–383CrossRefGoogle Scholar
  4. 4.
    Ansari PG, Rao DLN (2014) Differentiating Indigenous Soybean Bradyrhizobium and Rhizobium spp. of Indian Soils. Ind J Microbiol 54:190–195CrossRefGoogle Scholar
  5. 5.
    Li QQ, Wang ET, Zhang YZ, Zhang YM, Tian CF, Sui XH, Chen WF, Chen WX (2011) Diversity and biogeography of rhizobia isolated from root nodules of Glycine max grown in Hebei province, China. MicrobEcol 61:917–931Google Scholar
  6. 6.
    Zhang YM, Li Y Jr, Chen WF, Wang ET, Tian CF, Li QQ, Zhang YZ, Sui XH, Chen WX (2011) Biodiversity and biogeography of rhizobia associated with soybean plants grown in the north China plain. Appl Environ Microbiol 77(18):6331–6342CrossRefPubMedPubMedCentralGoogle Scholar
  7. 7.
    Chen LS, Figueredo A, Villani H, Michajluk J, Hungria M (2002) Diversity and symbiotic effectiveness of rhizobia isolated from field-grown soybean nodules in Paraguay. Biol Fertil Soils 35:448–457CrossRefGoogle Scholar
  8. 8.
    Ansari PG, Rao DLN, Pal KK (2014) Diversity and phylogeny of soybean rhizobia in central India. Ann Microbiol 64:1553–1565CrossRefGoogle Scholar
  9. 9.
    Hungria M, Vargas MAT (2000) Environmental factors affecting N2 fixation in grain legumes in the tropics, with an emphasis on Brazil. Field Crops Res 65:151–164CrossRefGoogle Scholar
  10. 10.
    Santos MA, Vargas MAT, Hungria M (1999) Characterization of soybean Bradyrhizobium strains adapted to the Brazilian savannas. FEMS Microbiol Ecol 30:261–272CrossRefPubMedGoogle Scholar
  11. 11.
    Somasegaran P, Hoben HJ (1994) Handbook for rhizobia: methods in legume-rhizobium technology. Springer, New YorkCrossRefGoogle Scholar
  12. 12.
    Dowdle SF, Bohlool BB, Berger CLR, Habte M, Silva JA, Singleton PW (1985) The ecology of Rhizobium japonicum in soybean-rice cropping systems in central china. In: Ph.D. thesis submitted to the graduate division of the University of Hawaii, ChinaGoogle Scholar

Copyright information

© The National Academy of Sciences, India 2018

Authors and Affiliations

  1. 1.Department of Soil Science and Agricultural ChemistryJawaharlal Nehru Krishi Vishwa VidyalayaJabalpurIndia
  2. 2.All India Network Project on Soil Biodiversity-BiofertilizersICAR-Indian Institute of Soil ScienceBhopalIndia

Personalised recommendations