National Academy Science Letters

, Volume 39, Issue 6, pp 465–467 | Cite as

Volatile Constituents of Nepeta ciliaris Benth. roots from Kumaun Himalayas

  • Shiv Shanker Gautam
  • Navneet
  • Sanjay Kumar
  • Deepak Painuly
  • Manindra Mohan
Short Communication
First Online:
Received:
Revised:
Accepted:
  • 122 Downloads

Abstract

The volatile constituents of Nepeta ciliaris Benth. roots were obtained by hydro-distillation and identified using Gas chromatography–Flame ionization detector (GC–FID) and Gas chromatography–Mass spectrometry (GC–MS) technique. Moreover, the antibacterial activity of crude oil has been carried out by using agar well diffusion method against five bacteria causing respiratory infections. Twenty compounds from essential oil of N. ciliaris were identified which represents 99.7 % of the total oil, consisting of oxygenated monoterpenoids (15.5 %), sesquiterpene hydrocarbons (77.4 %), oxygenated sesquiterpenoids (3.5 %) and diterpene hydrocarbons (3.3 %). The major constituents are α-ylangene (32.1 %), β-caryophyllene (16.1 %), guaiacol (11.3 %), α-humulene (8.1 %), isolongifolene (8.0 %), trans-α-bergamotene (4.5 %), and β-cyclocitral (3.0 %) while others were present in relatively small amounts. The antibacterial assay showed significant results against test bacterial strains.

Keywords

Nepeta ciliaris GC–MS Antibacterial activity Respiratory infection 
This is a preview of subscription content, log in to check access.

Notes

Acknowledgments

This work was supported by grant from UGC (University Grants Commission), New Delhi, India. The authors are grateful to the Head, Department of Botany and Microbiology, Gurukul Kangri University, Haridwar for providing the laboratory facilities to enable this research to be carried out and Botanical Survey of India, Northern Regional Center, Dehradun for plant identification.

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.

References

  1. 1.
    Hassan T, Rather MA, Shawl AS, Bhat KA, Bhat HM, Dar BA, Dar GH, Qurishi MA (2011) Chemical composition of the essential oils of Nepeta laevigata and Nepeta elliptica from India. Chem Nat Comp 47(3):456–458CrossRefGoogle Scholar
  2. 2.
    Formisano C, Rigano D, Senatore F (2011) Chemical constituents and biological activities of Nepeta species. Chem Biodivers 8:1783–1818CrossRefPubMedGoogle Scholar
  3. 3.
    Gkinis G, Tzakou O, Iliopoulou D, Roussis V (2003) Chemical composition and biological activity of Nepeta parnassica oils and isolated nepetalactones. Z Naturforsch C 58c:681–686Google Scholar
  4. 4.
    Bottini AT, Dev V, Garfagnoli DJ, Lohani H, Mathela CS, Pant AK (1987) (7R)-Trans, trans-nepetalactone from Nepeta elliptica. Phytochemistry 26(4):1200–1202CrossRefGoogle Scholar
  5. 5.
    Shafaghat A, Oji K (2010) Nepetalactone content and antibacterial activity of the essential oils from different parts of Nepeta persica. Nat Prod Commun 5(4):625–628PubMedGoogle Scholar
  6. 6.
    Grbic ML, Stupar M, Vukojevic J, Grubisic D (2011) Inhibitory effect of essential oil from Nepeta rtanjensis on fungal spore germination. Cent Eur J Biol 6(4):583–588Google Scholar
  7. 7.
    Adams RP (2009) Identification of essential oil components by gas chromatography/mass spectrometry, 4th edn. Allured Business Media, Carol StreamGoogle Scholar
  8. 8.
    Ahmad I, Mehmood Z, Mohammad F (1998) Screening of some Indian medicinal plants for their antimicrobial properties. J Ethnopharmacol 62:183–193CrossRefPubMedGoogle Scholar
  9. 9.
    Gilani AH, Shah AJ, Zubair A, Khalid S, Kiani J, Ahmed A, Rasheed M, Ahmad VU (2009) Chemical composition and mechanisms underlying the spasmolytic and bronchodilatory properties of the essential oil of Nepeta cataria L. J Ethnopharmacol 121:405–411CrossRefPubMedGoogle Scholar
  10. 10.
    Sefidkon F, Dabiri M, Alamshahi A (2002) Analysis of the essential oil of Nepeta fissa C.A. Mey. from Iran. Flavour Fragr J 17:89–90CrossRefGoogle Scholar
  11. 11.
    Mehrabani M, Asadipour A, Saber-Amoli S (2004) Chemical constituents of the essential oil of Nepeta depauperata from Iran. DARU J Pharm Sci 12:98–100Google Scholar
  12. 12.
    Javidnia K, Mehdipour AR, Hemmateenejad B, Rezazadeh SR, Soltani M, Khosravi AR, Miri R (2011) Nepetalactones as chemotaxonomic markers in the essential oils of Nepeta species. Chem Nat Comp 47(5):843–847CrossRefGoogle Scholar
  13. 13.
    Hadian J, Sonboli A, Ebrahimi SN, Mirjalili MH (2006) Essential oil composition of Nepeta satureioides from Iran. Chem Nat Comp 42:175–177CrossRefGoogle Scholar
  14. 14.
    Sefidkon F, Jamzad Z (2007) Essential oil composition of four Iranian Nepeta species (N. cephalotes, N. bornmuelleri, N. mirzayanii and N. bracteata). J Essent Oil Res 19:262–265CrossRefGoogle Scholar
  15. 15.
    Morteza-Semnani K, Saeedi M (2004) Essential oils composition of Nepeta cataria L. and Nepeta crassifolia Boiss. and Buhse from Iran. J Essent Oil-Bear Plants 7:120–122CrossRefGoogle Scholar
  16. 16.
    Sajjadi SE, Eskandari B (2005) Chemical constituents of the essential oil of Nepeta oxyodonta. Chem Nat Comp 41:175–177CrossRefGoogle Scholar
  17. 17.
    Kilic O, Hayta S, Bagci E (2011) Chemical composition of essential oil of Nepeta nuda L. subsp. Nuda (Lamiaceae) from Turkey. Asian J Chem 23(6):2788–2790Google Scholar

Copyright information

© The National Academy of Sciences, India 2016

Authors and Affiliations

  1. 1.Department of Botany and MicrobiologyGurukul Kangri UniversityHaridwarIndia
  2. 2.Department of ChemistryGurukul Kangri UniversityHaridwarIndia
  3. 3.Uttarakhand Council for Biotechnology Biotech BhavanHaldi Pantnagar, U.S. NagarIndia

Personalised recommendations