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National Academy Science Letters

, Volume 38, Issue 4, pp 301–304 | Cite as

Effect of Sulfuric Acid Treatment on Breaking of Seed Dormancy and Subsequent Seedling Establishment in Zanthoxylum armatum DC: An Endangered Medicinal Plant of the Himalayan Region

  • Sumit Purohit
  • S. K. NandiEmail author
  • L. M. S. PalniEmail author
  • Lalit Giri
  • Arun Bhatt
Short Communication

Abstract

An attempt has been made to influence seed germination in Zanthoxylum armatum DC, a medicinally important plant that produces seeds with hard coat and low natural regeneration. Pretreatment of seeds with concentrated (98 %) or diluted (50 %) sulfuric acid (H2SO4) for various time periods (1, 5, 10, 15, 20 and 25 min) and subsequent sowing in the soil (under polyhouse conditions) resulted in considerable improvement in germination as a result of some treatments. Treatment with diluted (50 %) H2SO4 (15 min) resulted in maximum germination (93.3 %) along with mean germination time (MGT) of 149.5 days. The other treatments (50 % H2SO4 for 5, 10, 20 and 25 min) also improved germination, but to a lesser extent (15–40 %) with MGT of 130–160 days. The values were significantly (P < 0.05) lower than the most effective treatment (50 % H2SO4, 15 min). No germination was recorded in other treatments including the untreated control. The growth of seedlings was monitored and showed rapid increase in height and leaf numbers with 100 % survival up to 2 years. The results clearly indicate a simple, inexpensive and convenient method of improving and obtaining uniform seed germination for nursery raising.

Keywords

Endangered Himalaya Seed germination Sulfuric acid Zanthoxylum armatum 

Notes

Acknowledgments

The authors are thankful to the Director, G.B. Pant Institute of Himalayan Environment and Development, Kosi-Katarmal, Almora, for facilities; Ministry of Environment & Forests, Govt. of India, New Delhi and UCOST, Dehradun are thanked for financial assistance.

References

  1. 1.
    Burnette R, Morikawa B (2006) Zanthoxylum propagation study in north Thailand. WWW.echocommuity.org/resource/collection/29B15DC2-7A23-4303-B93E−178AB6644D5F/EAN8_Thai.pdf
  2. 2.
    Hooker JD (1875) Flora of British India, vol I. L. Reeve & Company, London, p 740Google Scholar
  3. 3.
    Gaur RD (1999) Flora of the district Garhwal North Himalaya. Transmedia Publishers, Srinagar, p 811Google Scholar
  4. 4.
    Anonymous (1976) The wealth of india-a dictionary of indian raw materials & industrial products raw materials. Council of Scientific and Industrial Research, New Delhi, vol. 11 (X–Z), 11: 17–25Google Scholar
  5. 5.
    Chen IS, Wu SJ, Tsai IL (1994) Chemical and bioactive constituents from Zanthoxylum simulans. J Nat Prod 57:1206–1211CrossRefGoogle Scholar
  6. 6.
    Chen IS, Lin YC, Tsai IL, Teng CM, Ko FN, Ishikawa T, Ishii H (1995) Coumarins and anti-platelet aggregation constituents from Zanthoxylum schinifolium. Phtyochemistry 39:1091–1097CrossRefGoogle Scholar
  7. 7.
    Hisatomi E, Matsui M, Kobayashi A, Kubota K (2000) Antioxidative activity in the pericarp and seed of Japanese pepper (Xanthoxylum piperatum). J Agric Food Chem 48:4924–4928CrossRefGoogle Scholar
  8. 8.
    Jain N, Srivastava SK, Aggarwal KK, Ramesh S, Kumar S (2001) Essential oil composition of Zanthoxylum alatum seeds from northern India. Flavour Fragr J 16:408–410CrossRefGoogle Scholar
  9. 9.
    Kala CP (2010) Assessment of availability and patterns in collection of Timroo (Zanthoxylum armatum DC.): a case study of Uttarakhand Himalaya. Med Plants 2:91–96Google Scholar
  10. 10.
    Samant SS, Butola JS, Sharma A (2007) Assessment of conservation diversity, a status and preparation distribution of management plan for medicinal plants in the catchment area of Parbati hydroelectric project stage-III in northwestern Himalaya. J Mt Sci 4(1):34–56CrossRefGoogle Scholar
  11. 11.
    Frances A (2004) Seed storage characteristics and germination of south Florida native plant seeds. Fairchild Tropical Botanic Garden. http://www.ftg.org/PDF20Files/SeedStorageBehavior
  12. 12.
    Pandey H, Nandi SK, Nadeem M, Palni LMS (2000) Chemical stimulation of seed germination in Aconitum heterophyllum Wall. and A. balfouri Stapf.: important Himalayan species of medicinal value. Seed Sci Technol 28:39–48Google Scholar
  13. 13.
    Baes PO, Viana M, Silvia S (2002) Germination in Prosopis ferox seeds: effects of mechanical, chemical and biological scarificators. J Arid Environ 50:185–189CrossRefGoogle Scholar
  14. 14.
    Emongor VE, Mathowa T, Kabelo S (2004) The effect of hot water, sulphuric acid, nitric acid, gibberellic acid and ethephon on the germination of corchorus (Corchorus tridens) seed. J Agron 3:196–200CrossRefGoogle Scholar
  15. 15.
    Zida D, Tigabu M, Sawadogo L, Oden PC (2005) Germination requirement of seeds of four woody species from the Sudanian savanna in Burkina Faso, West Africa. Seed Sci Technol 33:581–593CrossRefGoogle Scholar
  16. 16.
    Tanaka-Oda A, Kenzo T, Fukuda K (2009) Optimal germination condition by sulfuric acid pretreatment to improve seed germination of Sabina vulgaris Ant. J For Res 14(4):251–256CrossRefGoogle Scholar
  17. 17.
    WAC (World Agroforesty Centre) (2005) Zanthoxylum gilletii from agro forestry tree database; a tree species reference and selection guide. www.Worldagroforesty.org/sea/products/AFDbase/af/asp/SpeciesInfo.asp?SpID=17988
  18. 18.
    Rincon-Rosales R, Culebro-Espinosa NR, Gutierrez-Miceli FA (2003) Scarification of seeds of Acacia angustissima (Mill.) Kuntze and its effect on germination. Seed Sci Technol 31:301–307CrossRefGoogle Scholar
  19. 19.
    Wang YR, Hanson J, Mariam YW (2007) Effect of sulfuric acid pretreatment on breaking hard seed dormancy in diverse accession of five wild Vigna species. Seed Sci Technol 35:550–559CrossRefGoogle Scholar
  20. 20.
    Baskin JM, Baskin CC (1998) Seeds, ecology, biogeography and evolution of dormancy and germination. Academic Press, California, p 668Google Scholar
  21. 21.
    Goo GH, Yoon KS, Choi JS (1993) Seed germination by Pon–Pon treatment and asexual multiplication by cuttings in Zanthoxylum piperitum. J Korean For Soc 82:227–234Google Scholar
  22. 22.
    Goo GH, Choi JS, Youn KS (1995) Effects of the seed treatment on field germination and seedling growth in four useful species, Euonymus alatus, Nandina domestica, Thea sinensis and Zanthoxylum piperitum. J Korean For Soc 84:87–96Google Scholar
  23. 23.
    Rodriguez L (1995) Tratamientos pregerminativos para algunas especies forestales natives, de la Reigon Huetar Norte De Costa Rica. Simposio Latino Americano sobre semillas forestales. 16–24 October, 1995. Managua, NicaraguaGoogle Scholar
  24. 24.
    Francis JK (2000) Zanthoxylum flavum Vahl. in Silvics of native and exotic trees of Puerto Rico and the Caribbean Islands. (Francis JK, Lowe CA eds.) United States Department of Agriculture Technical Report IITF-15. http://www.fs.fed.us/global/iitf/Zanthoxylumflavum.pdf

Copyright information

© The National Academy of Sciences, India 2015

Authors and Affiliations

  1. 1.G.B. Pant Institute of Himalayan Environment and DevelopmentAlmoraIndia
  2. 2.NainitalIndia
  3. 3.College of Forestry and Hill AgricultureTehri GarhwalIndia

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