National Academy Science Letters

, Volume 37, Issue 1, pp 5–12 | Cite as

High Frequency Organogenesis in Cotyledon and Hypocotyl Explants of Cabbage (Brassica oleracea L. var. capitata)

  • Shalini Sharma
  • Geetika Gambhir
  • D. K. Srivastava
Research Article
First Online:
Received:
Revised:
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Abstract

Morphogenetic potential of cotyledon and hypocotyl explants of cabbage (Brassica oleracea L. var. capitata) was studied to develop a reliable plant regeneration protocol. Nine days old aseptically grown seedlings of cabbage were used as source of explant for plant regeneration. Out of 36 combinations of growth regulators used in MS medium, high frequency shoot regeneration from cotyledon explant was obtained on MS medium supplemented with 1.5 mg/l BAP and 0.50 mg/l NAA and 2 mg/l BAP and 0.25 mg/l IAA respectively. High percentage root regeneration (90 %) in in vitro developed shoots were obtained on MS medium supplemented with 0.10 mg/l IBA. The regenerated complete plantlets were transferred to pots containing a mixture of sand and soil and acclimatized. A reproducible and efficient plant regeneration protocol has been standardized in cabbage cv. Pride of India.

Keywords

Acclimatization Cabbage Cotyledon Hypocotyl Indirect organogenesis In vitro regeneration 

Abbreviations

BAP

N 6-Benzylaminopurine

NAA

Naphthalene acetic acid

IAA

Indole-3-acetic acid

IBA

Indole-3-butyric acid

MS

Murashige and Skoog

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Notes

Acknowledgments

Authors thank to Professor and Head, Dept. of Vegetable Science of Dr. Y.S. Parmar University of Horticulture and Forestry, Solan, India for providing germplasm of cabbage cv. Pride of India.

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Copyright information

© The National Academy of Sciences, India 2014

Authors and Affiliations

  • Shalini Sharma
    • 1
    • 2
  • Geetika Gambhir
    • 2
  • D. K. Srivastava
    • 2
  1. 1.Dept. of BiotechnologyInstitute of Life Sciences and Business ManagementSolanIndia
  2. 2.Department of BiotechnologyDr Y.S. Parmar University of Horticulture and Forestry NauniSolanIndia

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