National Academy Science Letters

, Volume 35, Issue 4, pp 253–257 | Cite as

Pharmaco-Phylogenetic Investigation of Micromeria biflora Benth and Citrus reticulata Blanco

  • Awadhesh Kumar
  • Rita Gupta
  • Rohit K. Mishra
  • Amritesh C. ShuklaEmail author
  • Anupam Dikshit
Short Communication


Bacterial diseases, diarrhoea, cholera, typhoid and dysentery mainly originate through consumption of bacterial contaminated water. Comparative in-vitro antimicrobial activity of some essential oils (EOs) against a few water borne bacterial diseases are reported. The minimum inhibitory concentrations (MIC) of essential oil of Micromeria biflora Benth for Escherichia coli (ATCC-25922), E. coli (MTCC-723), Shigella dysenteriae (ATCC-23513) Salmonella typhimurium (MTCC-98) respectively was 0.172, 0.308, 0.178, 0.202 mg/ml but in case of Vibrio cholerae (MTCC-3906) the oil was found ineffective. The essential oil of Citrus reticulata Blanco had MIC against E. coli 2.60 mg/ml, V. cholerae 8.06 mg/ml, S. dysenteriae 2.99 mg/ml, S. typhimurium 5.18 mg/ml, but the oil has no efficacy against E. coli (MTCC). Further, due to synergistic activity, the oils were found to be more effective against MTCC strain of E. coli and V. cholerae at MIC of 0.15 and 0.14 mg/ml respectively. The phylogenetic evaluations of the pathogens were carried out with help of ClustalW computer program.


Water borne bacterial diseases Micromeria biflora Citrus reticulata Synergistic Phylogenetic 



Thanks to Department of Science and Technology (DST) for financial assistance; to Head, Department of Horticulture Aromatic and Medicinal Plants, Mizoram University, Aizawl as well as Head, Department of Botany, University of Allahabad for providing laboratory facilities.


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Copyright information

© The National Academy of Sciences, India 2012

Authors and Affiliations

  • Awadhesh Kumar
    • 1
  • Rita Gupta
    • 1
  • Rohit K. Mishra
    • 2
  • Amritesh C. Shukla
    • 1
    Email author
  • Anupam Dikshit
    • 2
  1. 1.Department of Horticulture, Aromatic and Medicinal PlantsMizoram UniversityAizawlIndia
  2. 2.Biological Product Laboratory, Department of BotanyUniversity of AllahabadAllahabadIndia

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