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Infection

pp 1–9 | Cite as

Genomic epidemiology of MRSA infection and colonization isolates among military trainees with skin and soft tissue infection

  • Eugene V. MillarEmail author
  • Gregory K. Rice
  • Carey D. Schlett
  • Emad M. Elassal
  • Regina Z. Cer
  • Kenneth G. Frey
  • Theron Hamilton
  • Michael W. Ellis
  • David R. Tribble
  • Kimberly A. Bishop-Lilly
  • Jason W. Bennett
Original Paper

Abstract

Purpose

Individuals with methicillin-resistant Staphylococcus aureus (MRSA) skin and soft tissue infection (SSTI) can be simultaneously colonized with MRSA on multiple body sites. Using whole genome sequencing (WGS), the intrahost relatedness of MRSA colonization and infection isolates was investigated.

Methods

In the context of a prospective case–control study of SSTI, we analyzed colonization and infection isolates from US Army Infantry trainees with purulent infection due to MRSA. At the time of clinical presentation for SSTI, culture swabs were obtained from the infection site, as well as from the patient’s nasal, oral, inguinal, and perianal regions. S. aureus culture and susceptibility was performed by standard methods. DNA from MRSA isolates was extracted and libraries were produced. Sequences were generated on an Illumina MiSeq, sequence reads were assembled, and single nucleotide variant (SNV) data were analyzed.

Results

Of 74 trainees with MRSA SSTI, 19 (25.7%) were colonized with MRSA. Ten (52.6%) were colonized on more than one body site. Colonization frequency by anatomic site was as follows: inguinal region (33%), nasal region (30%), perianal region (22%), and oral region (14%). A total of 36 MRSA colonization isolates were characterized. The intrahost median number of SNVs between infection and colonization isolates was 17. Among trainees with recurrent MRSA SSTI, limited intrahost diversity suggests that persistent colonization is a major contributor to recurrence risk.

Conclusions

Among military trainees with MRSA SSTI, genomic characterization of infection and colonization isolates revealed a high degree of strain relatedness. Single acquisition events may account for MRSA colonization and infection in this population.

Keywords

Methicillin-resistant Staphylococcus aureus (MRSA) Skin and soft tissue infection Colonization Whole genome sequencing Genomics Military 

Notes

Acknowledgements

We are indebted to the study team of clinical research coordinators, laboratory personnel, and data management staff for their dedication to the project.

Funding

This work was supported by a US Department of Defense Program Project Grant [HT9404-12-1-0019 to M.W.E.]. Additional support for this work was provided by the Department of Defense Global Emerging Infections Surveillance and Response System [GEIS;HU0001-10-1-0018 to M.W.E] and the Military Infectious Diseases Research Program [MIDRP; HT9404-12-1-0012 to M.W.E]. The protocol was conducted by the Infectious Disease Clinical Research Program (IDCRP), a Department of Defense (DoD) program executed through the Uniformed Services UNIVERSITY of the Health Sciences through a cooperative agreement with The Henry M. Jackson Foundation for the Advancement of Military Medicine, Inc. This project has been funded in part with federal funds from the National Institute of Allergy and Infectious Diseases, National Institutes of Health (NIH), under Inter-Agency Agreement Y1-AI-5072.

Compliance with ethical standards

Conflict of interest

All authors: no conflicts.

Disclaimer

The contents of this publication are the sole responsibility of the author(s) and do not necessarily reflect the views, opinions or policies of Uniformed Services University of the Health Sciences (USUHS), the Department of Defense (DoD), the Departments of the Army, Navy, or Air Force, or the Henry M. Jackson Foundation for the Advancement of Military Medicine. Mention of trade names, commercial products, or organizations does not imply endorsement by the US Government. Drs. Bennett, Bishop-Lilly, Hamilton, and Tribble are service members or employees of the U.S. Government. This work was prepared as part of their official duties. Title 17 U.S.C. §105 provides that ‘Copyright protection under this title is not available for any work of the United States Government.’ Title 17 U.S.C. §101 defines a U.S. Government work as a work prepared by a military service member or employee of the U.S. Government as part of that person’s official duties.

Supplementary material

15010_2019_1282_MOESM1_ESM.docx (204 kb)
Supplementary material 1 (DOCX 203 KB)

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Copyright information

© This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply 2019

Authors and Affiliations

  • Eugene V. Millar
    • 1
    • 2
    Email author
  • Gregory K. Rice
    • 3
    • 4
  • Carey D. Schlett
    • 1
    • 2
  • Emad M. Elassal
    • 1
    • 2
  • Regina Z. Cer
    • 3
    • 4
  • Kenneth G. Frey
    • 4
  • Theron Hamilton
    • 4
  • Michael W. Ellis
    • 5
  • David R. Tribble
    • 1
  • Kimberly A. Bishop-Lilly
    • 4
  • Jason W. Bennett
    • 6
    • 7
  1. 1.Infectious Disease Clinical Research Program, Department of Preventive Medicine and BiostatisticsUniformed Services University of the Health SciencesBethesdaUSA
  2. 2.Henry M. Jackson Foundation for the Advancement of Military MedicineRockvilleUSA
  3. 3.LeidosRestonUSA
  4. 4.Naval Medical Research Center, Biological Defense Research DirectorateFort DetrickUSA
  5. 5.University of Toledo College of Medicine and Life SciencesToledoUSA
  6. 6.Walter Reed Army Institute of ResearchSilver SpringUSA
  7. 7.Department of MedicineUniformed Services University of the Health SciencesBethesdaUSA

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