Infection

, Volume 40, Issue 6, pp 689–694 | Cite as

Dengue virus identification by transmission electron microscopy and molecular methods in fatal dengue hemorrhagic fever

  • D. Limonta
  • V. Falcón
  • G. Torres
  • V. Capó
  • I. Menéndez
  • D. Rosario
  • Y. Castellanos
  • M. Alvarez
  • R. Rodríguez-Roche
  • M. C. de la Rosa
  • A. Pavón
  • L. López
  • K. González
  • G. Guillén
  • J. Diaz
  • M. G. Guzmán
Case Report

Abstract

Dengue virus is the most significant virus transmitted by arthropods worldwide and may cause a potentially fatal systemic disease named dengue hemorrhagic fever. In this work, dengue virus serotype 4 was detected in the tissues of one fatal dengue hemorrhagic fever case using electron immunomicroscopy and molecular methods. This is the first report of dengue virus polypeptides findings by electron immunomicroscopy in human samples. In addition, not-previously-documented virus-like particles visualized in spleen, hepatic, brain, and pulmonary tissues from a dengue case are discussed.

Keywords

Dengue hemorrhagic fever Fatal Tissues Electron microscopy RT-PCR 

Notes

Acknowledgments

We are very grateful for the technical assistance and helpful comments during the manuscript preparation of Prof. Maritza Pupo from the Virology Department at the Pedro Kourí Tropical Medicine Institute (IPK). We acknowledge the staff of the Pathology Department and Intensive Care Unit of the Salvador Allende General Hospital in Havana. We are also grateful of Gilda Lemus from the Diagnostics Department of the Center for Biological Studies (CIB) in Havana for providing the anti-NS1 polyclonal antibodies.

Conflict of interest

The authors declare that there is no conflict of interest.

Ethical standards

This study received approval from the Ethics Committee of Pedro Kourí Tropical Medicine Institute (IPK).

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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • D. Limonta
    • 1
  • V. Falcón
    • 2
  • G. Torres
    • 3
  • V. Capó
    • 3
  • I. Menéndez
    • 2
  • D. Rosario
    • 1
  • Y. Castellanos
    • 1
  • M. Alvarez
    • 1
  • R. Rodríguez-Roche
    • 1
  • M. C. de la Rosa
    • 2
  • A. Pavón
    • 1
  • L. López
    • 3
  • K. González
    • 4
  • G. Guillén
    • 5
  • J. Diaz
    • 1
  • M. G. Guzmán
    • 1
  1. 1.Arbovirus Laboratory, Virology Department, PAHO/WHO Collaborating Center for the Study of Dengue and its VectorPedro Kourí Tropical Medicine Institute (IPK)HavanaCuba
  2. 2.Electron Microscopy LaboratoryCenter for Genetic Engineering and Biotechnology (CIGB)HavanaCuba
  3. 3.Pathology DepartmentPedro Kourí Tropical Medicine Institute (IPK)HavanaCuba
  4. 4.Pathology DepartmentSalvador Allende General HospitalHavanaCuba
  5. 5.Biomedical Research DivisionCenter for Genetic Engineering and Biotechnology (CIGB)HavanaCuba

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