Infection

, Volume 30, Issue 2, pp 86–89 | Cite as

Serum Citrate Levels, Haptoglobin Haplotypes and Transferrin Receptor (CD71) in Patients With HIV-1 Infection

  • A. Pugliese
  • L. Gennero
  • G. P. Pescarmona
  • M. Beccattini
  • E. Morra
  • G. Orofino
  • D. Torre
Clinical and Epidemiological Study

Abstract

Background: The progression of HIV-1 infection towards its more advanced stages is accompanied by changes in iron metabolism and increased body iron stores.

Patients and Methods: Given the ability of HIV to alter iron metabolism, we studied the principal (transferrin system) and alternative (citrate system) iron pathways in a group of 65 HIV-infected patients (symptomatic stage B1–B3) and in a group of 36 healthy seronegative individuals. We determined serum citrate levels, haptoglobin (Hp) haplotypes, expression of transferrin receptor (CD71) on cell lines infected with HIV-1 as well as iron markers including blood iron, transferrin and ferritin.

Results: Our data showed decreased serum citrate levels in the HIV-infected patients compared to controls (92.9 ± 22.4 μM/l vs 126.2 ± 29.2 μM/l; p < 0.01). In particular, the serum citrate levels negatively correlated with HIV-1 RNA copy number (mean: 2.53 ± 1.88 × 105/ml, rs = 0.70, p < 0.01) and positively correlated with CD4+ T-lymphocyte count (mean: 241 ± 168/ml, rs = 0.64, p > 0.05). Accordingly, blood iron, transferrin and red cell concentrations were lower in HIV-infected patients compared to the controls, whereas serum ferritin levels were higher in HIV-infected patients. Moreover, the Hp haplotype distribution showed significant differences only in the group of HIV-infected patients (p = 0.02; χ2 test).

Conclusion: Our results show that iron metabolism is altered in patients with HIV-1 infection. The alternative pathway (citrate system) is particularly affected, since when citrate levels are low, both aconitase activity and HIV-1 replication need iron.

Key Words Serum citrate Haptoglobin Blood iron HIV-1 infection 

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Copyright information

© Urban & Vogel Medien und Medizin Verlagsgesellschaft 2002

Authors and Affiliations

  • A. Pugliese
    • 1
  • L. Gennero
    • 1
  • G. P. Pescarmona
    • 2
  • M. Beccattini
    • 2
  • E. Morra
    • 2
  • G. Orofino
    • 3
  • D. Torre
    • 4
  1. 1.Dept. of Medical Surgery, Infectious Disease Section, University of Turin, Turin, ItalyItaly
  2. 2.Dept. of Genetics, Biology and Biochemistry, Biochemistry Section, University of Turin, Turin, ItalyItaly
  3. 3.Infectious Disease Division, Amedeo di Savoia Hospital, Turin, ItalyItaly
  4. 4.Infectious Disease Division, Hospital di Circolo, Viale Borri 57, I-21100 Varese, Italy; Phone (+39/332) 278446, Fax: -8580, e-mail: eiwle@tin.itItaly

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