Local bivalve allergens — a Malaysian perspective
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Apart from the nutritional benefits, bivalves are also considered to be among a group of allergenic foods. Symptoms of allergy to bivalves are largely similar to those of allergic reactions reported for other shellfish. The identification of allergenic proteins in a particular species is an important step for the development of more accurate allergy tests and for the definition of more effective management of patients. Currently, studies regarding the allergens in bivalve species are lacking.
Materials and methods
This minireview is an overview of local scientific articles on identification of bivalve allergens by allergenomics methods including sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), two-dimensional gel electrophoresis (2-DE), immunoblotting and mass-spectrometry analysis.
Tropomyosin has been the only well recognized allergen in a wide range of bivalve species. In addition to tropomyosin, our local study resulted in discovery of three novel bivalve major allergens; arginine kinase (40 kDa), actin (42 kDa) and troponin C (18 kDa).
This minireview will provide a better understanding of each allergen derived from local bivalves which subsequently will help in the understanding of the specific immunoglobulin E(IgE)-mediated immune response.
Schlüsselwörterallergen bivalve tropomyosin arginine kinase actin troponin C mollusca
Two dimensional-gel electrophoresis
Complementary deoxyribonucleic acid
Enzyme-linked immunosorbent assay
Sodium dodecyl sulfate polyacrylamide-gel electrophoresis
- 4.Laxmilatha P. Proximate composition of the surf clamMactraviolacea (Gmelin 1791). Indian J Fish 2009;56:147–50Google Scholar
- 6.Department of Fisheries Malaysia (DOF). 2015. Rectified. (Annual Fisheries Statistics 2015). http://www.dof.gov.my. Accessed 20 Apr 2016
- 7.European Food Safety Authority (EFSA). Opinion of the scientific panel on dietetic products, nutrition and allergies on a request from theCommission related to the evaluation ofmollusks forlabelingpurposes. EFSA J 2006;327:1–25Google Scholar
- 8.Food and Agriculture Organization of the United Nations (FAO), World Health Organization (WHO). Evaluation of allergenicity of genetically modified foods. In: Report of a joint FAO/WHO expert consultation of allergenicity of foodsderived frombiotechnology. 2001. pp. 22–5Google Scholar
- 11.Castillo R, Delgado J, Quiralte J, Blanco C, Carillo T. Food hypersensitivity among adult patients: epidemiological andclinical aspects. Allergol Immunopathol 1996;24:93–7Google Scholar
- 12.Zinn C, Lopata A, Visser M, Potter PC. The spectrum of allergy to South African bony fish (Teleosti). Evaluation by double-blind, placebo-controlled challenge. S Afr Med J 1997;87:146–52Google Scholar
- 13.Tomikawa M, Suzuki N, Urisu A, Tsuburai T, Ito S, Shibata R, et al. Characteristics of shrimp allergy from childhood to adulthoodin Japan. Arerugi 2006;55:1536–42Google Scholar
- 14.Arai Y, Sano Y, Ito K, Iwasaki E, Mukouyama T, Baba M. Food and food additives hypersensitivity in adult asthmatics. Skin scratch test with food allergens and food challenges in adult asthmatics. Arerugi 1998;47:658–66Google Scholar
- 19.Erikson NE, Moller C, Werner S, Magnusson J, Bengtsson U, Zoloubas M. Self-reported foodhypersensitivityin Sweden, Denmark, Estonia, Lithuania and Russia. J Investig Allergol Clin Immunol 2004;14:70–9Google Scholar
- 20.Zailatul HMY. Production,characterization andcross-reactivity of local bivalve allergens. PhD Thesis 2017, Universiti Pendidikan Sultan IdrisGoogle Scholar
- 24.Rossi RE, Melioli G, Monasterolo G, Harwanegg C, Rossi L, Canonica GW, et al. Sensitization profiles in polysensitized patients froma restrictedgeographicalarea: furtherlessons from multiplexed component resolved diagnosis. Eur Ann Allergy Clin Immunol 2011;43:171–5Google Scholar
- 28.Chu KH, Wong SH, Leung SC. Tropomyosin is the major mollusc allergen: reverse transcriptase polymerase chain reaction, expression and IgE reactivity. Mar Biotechnol 2000;2:499–509Google Scholar
- 31.Rosmilah M, Shahnaz M, Zailatul HM, Noormalin A, Normilah I. Identification of tropomyosin and arginine kinase as major allergens of Portunus pelagicus (blue swimming crab). Trop Biomed 2012;29:467–78Google Scholar
- 33.Yadzir ZH, Misnan R, Murad S. Identification of tropomyosin as major allergen of white squid (Loligo edulis) by twodimensional immunoblotting and mass spectrometry. Southeast Asian J Trop Med Public Health 2012;43:185–91Google Scholar
- 36.Zailatul HMY, Rosmilah M, Faizal B, Noormalin A, Shahnaz M. Malaysian cockle (Anadara granosa) allergy: identification of IgE-binding proteins and effects of different cooking methods. TropBiomed 2015;32:323–34Google Scholar
- 37.Yadzir ZHM, Misnan R, Bakhtiar F, Abdullah N, Murad S. Tropomyosin and actin identified as major allergens of the carpet clam (Paphia textile) and the effect of cooking on their allergenicity. Biomed Res Int 2015;2015:254152. https://doi.org/10.1155/2015/254152Google Scholar
- 39.Yadzir ZHM, Misnan R, Bakhtiar F, Abdullah N, Abdullah H, Murad S. Identification of tropomyosin and troponin C as the major allergens of Perna viridis (Asian green mussel) and the effect of cooking on their allergenicity. Curr Allergy Clin Immunol 2017;30:30–5Google Scholar